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PARALLEL ORAL SESSIONS IV – POS.IV.3 THURSDAY, FEB. 21 IV.3 - LIFE & HEALTH III Laura Oliveira & Marcela Segun<strong>do</strong> Screening of Phenolic compounds with Bioactivity in Pieris brassicae L. Reared on Brassica rapa 27 var rapa L. using HPLC-DAD-MS/MS-ESI D. M. Pereira, A. Noites, F. Ferreres, P. Valentão, R. M. Seabra, P. B. Andrade Spatio-temporal characterization of the En<strong>do</strong>cannabinoid System enzymes-FAAH and COX-2 in the 69 fetoplacental unit A. Leite, B. Fonseca, N.A. Teixeira, G. Correia da Silva Determination of hypoxanthine in vitreous humor by a sequential injection analysis system and its 84 application in the estimation of postmortem interval Ana M. Santos, Ana I. Pereira, Marieta L. C. Passos, S. Frazão, Agostinho J.C. Santos, M. Lúcia M. F. S. Saraiva, José L. F. C. Lima A new method to evaluate spontaneous pain in neuropathic pain models: a study in rats with spared 87 nerve injury A. Sequeira, I. Martins, S. Araújo, D. Lima, I. Tavares Comprehensibility of pictorial representations within health educational and instructional artifacts - a 167 case study E. Ferreira, S. Coutinho 212 Calcium uptake by rat hippocampal synaptosomes: regulation by different depolarizing agents A. Rocha, M.G.B. Lobo, and P. Correia-de-Sá 226 New Conditions for Greater Purification Yield of Phosphorylated Period Protein in Drosophila Christine M. Lomiguen, Isaac Edery
Screening of Phenolic compounds with Bioactivity in Pieris brassicae L. Reared on Brassica rapa var rapa L. using HPLC- DAD-MS/MS-ESI D. M. Pereira 1 , A. Noites 1 F. Ferreres 2 P. Valentão 1 , R. M. Seabra 1 and P. B. Andrade 1 1 REQUIMTE/ Department of Pharmacognosy, Faculty of Pharmacy, University of Oporto, Portugal email: david.ffup@gmail.com 2 Research Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS (CSIC), Murcia, Spain In this research we proceeded to the study of Pieris brassicae, a frequent pest of some species, as a source of compounds with interest for health. The phenolic profiles of P. brassicae at different development stages (larvae, exuviae and butterfly), its excrements and its host plant, Brassica rapa var. rapa L., were determined by HPLC-DAD-MS/MS- ESI (Fig.1). Twenty five acylated and nonacylated flavonoid glycosides, as well as ferulic and sinapic acids, were identified in host plant, from which only twelve compounds were found in the excrements. In addition, the excrements showed the presence of sulphate flavonoids and other flavonoid glycosides, not detected in the leaves of the host plant. In the larvae kept without food for twelve hours, only three compounds common to the plant material and two others also present in the excrements were characterized. Brassica rapa var rapa Pieris brassicae Fig. 1. Detection at 330 nm. (1) kaempferol-3-O-(methoxycaffeoyl)sophoroside-7-O-glucoside; (2) quercetin-3-O- (sinapoyl)sophoroside-7-O-glucoside; (3) quercetin-3-O-(feruloyl)sophoroside-7-O-glucoside; (4) quercetin-3-O- (p-coumaroyl)sophoroside-7-O-glucoside; (5) kaempferol-3-O-(sinapoyl)sophoroside-7-O-glucoside; (6) kaempferol-3-O-(feruloyl)sophoroside-7-O-glucoside; (7) kaempferol-3-O-(p-coumaroyl)sophoroside-7-Oglucoside; (8) p-coumaric acid; (9) kaempferol-3,7-di-O-glucoside; (10) isorhamnetin-3,7-di-O-glucoside; (11) ferulic acid; (12) sinapic acid; (13) kaempferol-3-O-(feruloyl)sophoroside-7-O-glucoside (isomere); (14) kaempferol-3-O-(methoxycaffeoyl)sophoroside; (15) kaempferol-3-O-(caffeoyl)sophoroside; (16) kaempferol-3- O-sophoroside; (17) kaempferol-3-O-(feruloyl)sophoroside; (18) kaempferol-3-O-glucoside; (19) isorhamnetin-3- O-glucoside.(20) quercetin-3-O-sophoroside; (21) kaempferol-3-O-(p-coumaroyl)sophoroside (isomere). Antioxidant potential was studied against DPPH . radical and a reactive oxygen species (superoxide radical). The three samples showed an effective activity against both radicals in a concentration-dependant manner, with larvae displaying the strongest capacity against DPPH . , while excrements were the most effective superoxide radical scavenger. So, the matrices studied herein, may constitute a promising source of bioactive complex compounds, hard to be isolated or synthesized in the laboratory due to its high structural complexity. 98
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IJUP08 - Universidade do Porto

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