IJUP08 - Universidade do Porto

HPLC-DAD-MS/MS analysis of phenolics in vitro shoots of
Brassica oleracea L. var. costata DC
M. Taveira 1 , C. Sousa 1 , F. Ferreres 2 , D. M. Pereira 1 , Paula B. Andrade 1 , R. M.
Seabra 1 , Pedro Marques 1 , Patrícia Valentão 1
1 REQUIMTE/Department of Pharmacognosy, Faculty of Pharmacy, University of Oporto, Oporto,
Portugal. marcos.taveira@gmail.com
2 Research Group on Quality Safety and Bioactivity of Plant Foods, Department of Food Science
and Technology, CEBAS (CSIC), P.O Box 164, 30100 Campus University of Espinardo, Murcia,
Spain.
Brassica oleracea is native of the Mediterranean region and southwestern Europe, with a
great importance in human nutrition. Brassica species, including B. oleracea, are reported
to possess antioxidant activity, which is partially attributed to their phenolic compounds.
In vitro culture of plants is a method of biomass production, which enables production of
large number of species in a short period of time and space. Different conditions and
supplements used in medium constitution affect plant viability, growing and production of
specific metabolites.
For the first time, phenolic compounds produced by in vitro shoots of B. oleracea L. var.
costata DC were determined by HPLC-DAD-MS/MS (Fig 1). Thirty seven compounds
were characterized, which included chlorogenic acids, flavonoids (the majority of them
hydroxycinnamic acid esters of kaempferol and quercetin glycosides) and
hydroxycinnamic acyl glycosides (with predominance of synapoyl gentiobiosides).
320nm
Fig 1. Peaks: (1) 3-caffeoylquinic acid; (2) 3-p-coumaroylquinic acid; (3) 3-feruloylquinic acid; (4) 4caffeoylquinic
acid; (5) kaempferol-3-O-(caffeoyl)-sophorotrioside-7-O-glucoside; (6) caffeoylferuloylquinic acid;
(7) quercetin-3-O-(sinapoyl)-sophorotrioside-7-O-glucoside; (8) synapoyl glucoside; (9) kaempferol-3-O-
(sinapoyl)-sophorotrioside-7-O-sophoroside; (10) kaempferol-3-O-(sinapoyl)-sophorotrioside-7-O-glucoside; (11)
4-p-coumaroylquinic acid; (12) kaempferol-3-O-(feruloyl)-sophorotrioside-7-O-glucoside; (13) disinapoylgentiobioside;
(14) kaempferol-3-O-(sinapoyl)-sophorotrioside-7-O-rhamnoside; (15) quercetin-3-O-(sinapoyl)sophorotrioside;
(16) kaempferol-3-O-(methoxycaffeoyl)-sophorotrioside; (17) kaempferol-3-O-(feruloyl)sophorotrioside-7-O-rhamnoside;
(18) quercetin-3-O-sophoroside; (19) sinapoyl,caffeoyl,feruloyl-gentiobioside;
(20) kaempferol-3-O-(caffeoyl)-sophorotrioside; (21) kaempferol-3-O-(sinapoyl)-sophorotrioside; (22)
kaempferol-3-O-(feruloyl)-sophorotrioside; (23) kaempferol-3-O-sophoroside; (24) kaempferol-3-O-
(methoxycaffeoyl/sinapoyl)-sophorotrioside-7-O-sophoroside; (25) sinapoyl,methoxycaffeoyl-gentiobioside; (26)
kaempferol-3-O-(methoxicaffeoyl/sinapoyl)-sophorotrioside-7-O-glucoside; (27) kaempferol-3-O-
(caffeoyl/sinapoyl)-sophorotrioside-7-O-sophoroside; (28) kaempferol-3-O-(caffeoyl/sinapoyl)-sophorotrioside-7-
O-glucoside; (29) quercetin-3-O-(disinapoyl)-sophorotrioside-7-O-glucoside; (30) disinapoyl,methoxycaffeoylgentiobioside;
(31) disinapoyl,caffeoyl-gentiobioside; (32) kaempferol-3-O-(disinapoyl)-sophorotrioside-7-Oglucoside;
(33) disinapoyl-gentiobioside isomer; (34) sinapoyl,feruloyl-gentiobioside; (35) trisinapoylgentiobioside;
(36) disinapoyl,feruloyl-gentiobioside; (37) sinapoyl,diferuloyl-gentiobioside.
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