IJUP08 - Universidade do Porto

More documents

Recommendations

Info

PARALLEL ORAL SESSIONS II – POS.II.1 THURSDAY, FEB. 21 II.1 - FOOD & HEALTH Maria Paula Santos & Jorge Teixeira 60 Microplate high-throughput methodology for determination of Folin-Ciocalteu index F. Santos, L. M. Magalhães, M. A. Segundo, S. Reis, J. L. F. C. Lima 100 Detection of authorized genetically modified maize events: Participation in an inter-laboratorial study J. Rocha, I. Mafra, M. Carvalho, J.A. Amaral, M.B.P.P. Oliveira 101 Detection of genetically modified soybean in foodstuffs: Participation in an inter-laboratorial study M. Carvalho, I. Mafra, J. Rocha, M.B.P.P. Oliveira HPLC separation and quantification of Catechins in green tea home prepared: comparison with 160 commercial soft drinks C. Petisca, A. Melo, O. Pinho, I.M.P.L.V.O. Ferreira 182 Product Engineering - Application of vanillin in alimentary food paste industry A. Vasconcelos, B. Moura, L. Almeida, S. Couto 200 Development of a coating barrier for cork stoppers for their use in spirituous drinks F. Oliveira, S. Pontes, M. Cabral, A. Mendes
Microplate high-throughput methodology for determination of Folin-Ciocalteu index F. Santos, L. M. Magalhães, M. A. Segundo, S. Reis and J. L. F. C. Lima REQUIMTE, Serviço de Química-Física, Faculdade de Farmácia, Universidade do Porto, Rua Aníbal Cunha, 164, 4099-030, Porto, Portugal Email: miguel.santos.2@gmail.com The interest in antioxidant nutrients has increased due to their protective effects against free radical-induced reactions [1]. In this context, the Folin-Ciocalteu assay [2] has been proposed recently as a standard method for measurement of antioxidant capacity of food products and dietary supplements in routine quality control [3]. Therefore, the main goal of this work is the development of a simple, fast and reliable analytical methodology for the determination of Folin-Ciocalteu index. To achieve this objective, the conventional methodology was adapted to a 96-well microplate format. The reaction volume was then reduced 100 times, from 20 mL to 0.2 mL. Furthermore, the reaction conditions were thoroughly studied, namely the pH of the reaction media. It was observed that an increase of pH from 10 (usual value applied, provided by a carbonate buffer) to values around 12-13 changed the reaction kinetics dramatically. In fact it was possible to decrease the reaction time from 2 hours to 3-5 minutes. The developed methodology was applied to the determination in several types of food products, using gallic acid as standard compound. The Folin-Ciocalteu index values obtained for wines, beers and juices were comparable to those obtained from the previous batch method, with a significant improvement in the reagent consumption, effluent produced per assay and determination throughput. These characteristics constitute a step forward the implementation of greener procedures in the Analytical Chemistry area. References: [1] Halliwell, B., Murcia, M.A., Chirico, S., and Aruoma, O.I. (1995), Free-radicals and antioxidants in food and in vivo –what they do and how they work, Critical Reviews in Food Science and Nutrition, 35 (1-2), 7-20. [2] Singleton, V.L., Orthofer, R., and Lamuela-Raventós, R.M. (1999), Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent, Methods in Enzymology, 299, 152-178. [3] Prior, R.L., Wu, X., and Schaich, K. (2005), Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements, Journal of Agricultural and Food Chemistry, 53 (10), 4290-4302. 42
Page 1 and 2:
IJUP08 First Meeting of Young Resea
Page 3 and 4:
Adrián Silva Dinis Cayolla Ribeiro
Page 5 and 6: PERMEABILITY CHARACTERISTICS OF HIG
Page 7 and 8: THE COURTYARD AND SIZA’S HOUSES__
Page 9 and 10: INTRACELLULAR GENE REGULATION VIA E
Page 11 and 12: COPPER(II)/CIPROFLOXACIN COMPLEXES:
Page 13 and 14: INFLUENCE OF BEER ANTIOXIDATIVE PRO
Page 15 and 16: INFORMATION SYSTEMS ABOUT MATERIALS
Page 17 and 18: 8:30 8:45 8:45 9:00 9:00 9:15 9:15
Page 19 and 20: INTERDISCIPLINARY ORAL SESSIONS - I
Page 21 and 22: Construction of a learning unit in
Page 23 and 24: Angiogenesis modulation by catechin
Page 25 and 26: Alfonso X’s General Estoria and t
Page 27 and 28: Alternative User Interfaces for Gam
Page 29 and 30: Treatment of Myasthenia gravis with
Page 31 and 32: Automatic Emotion Assessment throug
Page 33 and 34: Permeability Characteristics of Hig
Page 35 and 36: PARALLEL ORAL SESSIONS I - POS.I.1
Page 37 and 38: Antitumor and antifungal activities
Page 39 and 40: Synthesis and Determination of Phys
Page 41 and 42: Synergism between adenosine A2A and
Page 43 and 44: The knowledge-based economy as a he
Page 45 and 46: The Space of Intervals. The inner s
Page 47 and 48: Interactive Application for Spatial
Page 49 and 50: PARALLEL ORAL SESSIONS I - POS.I.3
Page 51 and 52: The dynamical state of galaxies: fr
Page 53 and 54: Anti-de Sitter space and the stabil
Page 55: Quantum Vacuum Energy in General Re
Page 59 and 60: Detection of genetically modified s
Page 61 and 62: Product Engineering - Application o
Page 63 and 64: PARALLEL ORAL SESSIONS II - POS.II.
Page 65 and 66: Organic living | Weekend house in C
Page 67 and 68: Casa Comum [common/collective/commu
Page 69 and 70: The silence and the sky: seclusion
Page 71 and 72: Effect of metals on the AtPreP1 pep
Page 73 and 74: Anchoring of macrocycle compounds o
Page 75 and 76: Functional Insulin Quantification u
Page 77 and 78: PARALLEL ORAL SESSIONS III - POS.II
Page 79 and 80: MUC1 overexpression is associated w
Page 81 and 82: Prenylated and bromoalkylated xanth
Page 83 and 84: Impairment of retrograde signalling
Page 85 and 86: Contemporary condition: ‘new’ r
Page 87 and 88: Architecture and climate. Site geog
Page 89 and 90: Incorporation of organics/inorganic
Page 91 and 92: PARALLEL ORAL SESSIONS III - POS.II
Page 93 and 94: Exhibiting Architecture in Portugue
Page 95 and 96: Promenade to Corbusier L. Ribeiro d
Page 97 and 98: CONTACT POINTS_BETWEEN SWISS AND PO
Page 99 and 100: Levels of Butyltins in Sediment and
Page 101 and 102: Azo-dye Orange II degradation by Fe
Page 103 and 104: Isolation and characterisation of a
Page 105 and 106: PARALLEL ORAL SESSIONS IV - POS.IV.
Page 107 and 108:
Natural resources course B. Magalh
Page 109 and 110:
Desistência do tratamento de enure
Page 111 and 112:
A troubled legacy. Changing strateg
Page 113 and 114:
Screening of Phenolic compounds wit
Page 115 and 116:
Determination of hypoxanthine in vi
Page 117 and 118:
Comprehensibility of pictorial repr
Page 119 and 120:
New Conditions for Greater Purifica
Page 121 and 122:
28 HPLC-DAD-MS/MS analysis of pheno
Page 123 and 124:
PHOTOCATALYTIC DEGRADATION OF CIPRO
Page 125 and 126:
209 Evaluation of genetic and chemi
Page 127 and 128:
Thermodynamic and Theoretical Study
Page 129 and 130:
Possible correlation between morbid
Page 131 and 132:
Determination of Cadmium Content in
Page 133 and 134:
Optimization of an enzymatic and ch
Page 135 and 136:
Low-cost materials as adsorbents fo
Page 137 and 138:
An expeditious method for determina
Page 139 and 140:
Effect of pyrophosphate on firefly
Page 141 and 142:
Touristic Topographies : transversa
Page 143 and 144:
Copper(II)/Ciprofloxacin complexes:
Page 145 and 146:
Feeling Architecture. The importanc
Page 147 and 148:
Authenticity evaluation and molecul
Page 149 and 150:
Computational Analisys of Cynara ca
Page 151 and 152:
Chromium remediation using zero val
Page 153 and 154:
Potentiometric determination of gen
Page 155 and 156:
DFT STUDY ON THE ABILITY OF CALIX[2
Page 157 and 158:
Steady state and lifetime chemical
Page 159 and 160:
Scopoletin, a natural coumarin isol
Page 161 and 162:
New approaches for estimating the p
Page 163 and 164:
Firing distance estimation through
Page 165 and 166:
Synthesis, structure elucidation an
Page 167 and 168:
Ultrastructural characterisation of
Page 169 and 170:
Physical exercise and stress hormon
Page 171 and 172:
PHOTOCATALYTIC DEGRADATION OF CIPRO
Page 173 and 174:
Geometry and Space Conception the i
Page 175 and 176:
Formation of heterocyclic aromatic
Page 177 and 178:
THE STUDY OF DE FÂNZERES-GONDOMAR
Page 179 and 180:
Resonant frequency shift / Hz Quart
Page 181 and 182:
Synthesis of xanthone derivatives b
Page 183 and 184:
MeO Synthesis, Structural Elucidati
Page 185 and 186:
Primary Hyperparathyroidism: the un
Page 187 and 188:
Effects of etoposide, doxorubicin a
Page 189 and 190:
Characterization of exotic wood spe
Page 191 and 192:
Interactions of sulindac and its me
Page 193 and 194:
The Japanese Influence in Modern We
Page 195 and 196:
Conduction block in isolated rat sc
Page 197 and 198:
Monitoring particulate matter using
Page 199 and 200:
Education and Poverty: an empowerme
Page 201 and 202:
Characterization of Urea-Formaldehy
Page 203 and 204:
Investigation of the Interactions b
Page 205 and 206:
Adenosine regulates differentiation
Page 207 and 208:
Pravastatin Quantification using Sq
Page 209 and 210:
Information Systems about Materials
Page 211 and 212:
Potential of Equisetum arvense hydr
Page 213 and 214:
Taxonomy from rural diffuse to urba
Page 215 and 216:
Study and design of precast reinfor
Page 217 and 218:
Influence of egg yolk and aromatizi
Page 219 and 220:
The metamorphosis of Aleixo towers
Page 221 and 222:
Screening for novel CDH1 inactivati
Page 223 and 224:
Genetic characterization by SSR of
Page 225 and 226:
PLANTAS DO EDIFÍCIO DA FACULDADE D
Page 227 and 228:
EDIFICIO D 2D19-1 D.T. 2D63-1 C1 2D
show all

IJUP08 - Universidade do Porto

Create successful ePaper yourself

Delete template?

Save as template?