IJUP08 - Universidade do Porto

More documents

Recommendations

Info

PARALLEL ORAL SESSIONS II – POS.II.3 THURSDAY, FEB. 21 II.3 - NANO & SUPRAMOLECULAR CHEMISTRY Marcela Segundo & Adrián M.T. Silva 35 Effect of metals on the AtPreP1 peptidasome activity J. Pessoa, H.G. Bäckman, S. Bhushan, E. Glaser 38 Liposome-incorporated Zanthoxylum tingoassuiba essential oil: preparation and characterization C. Detoni, B. Sarmento, D. Ferreira, E.C.M. Cabral-Albuquerque 90 Anchoring of macrocycle compounds onto nanostructured carbon materials for catalytic applications Dalila Teixeira, Delfina Barros, Susana L. H. Rebelo, M. F. R. Pereira, J. J. M. Órfão, Cristina Freire 132 Characterizing the intermetallic formed during ball attach process Paulo Pereira, Rúben Santos, Maria M. Barbosa, Cátia Almeida 134 Functional Insulin Quantification upon its Nanoencapsulation M. J. Barbosa, J. Faria, O. Queirós, A. Ribeiro, R. Moreira Temperature and composition effects on the micelle formation by a catanionic surfactant: a surface 142 tension and conductivity study R. F. Fernandes, B. F. B. Silva, E. F. Marques
Effect of metals on the AtPreP1 peptidasome activity J. Pessoa 1,2 , H.G. Bäckman 2 , S. Bhushan 2 and E. Glaser 2 1 Institute for Molecular and Cell Biology, Faculty of Sciences, University of Porto, Portugal. 2 Department of Biochemistry and Biophysics, Faculty of Science, University of Stockholm, Sweden. In eukaryotic cells, proteins are targeted to their correct organelle by targeting peptides, which are amino acid extensions located in the N-terminal portion of the precursor protein. After the protein has reached its final intracellular destination, targeting peptides are cleaved off, and because of their cell toxicity, they must be removed. A novel metalloprotease that efficiently degrades targeting peptides in mitochondria and chloroplasts was identified by our group, and named Presequence Protease, PreP [1]. In Arabidopsis thaliana there are two isoforms of PreP (AtPreP1 and AtPreP2) that show an 86% homology at the amino acid sequence level [2]. AtPreP1 is composed of 995 amino acids, and contains a zinc-binding motif in its catalytic site. It also contains two magnesium-binding sites, one located on the inner side of the peptide binding cavity and another on the outer surface of the enzyme [3]. Magnesium (or calcium) has been shown to be needed for catalysis. The aim of this project is to understand the role of magnesium for the degradation activity. For that, the activity of AtPreP1 variants (which are unable to bind magnesium) was measured and compared to the wild-type. Three AtPreP1 variants, one in which both magnesium-binding sites were changed, and two in which only one magnesium-binding site was altered, were generated, overexpressed in bacteria and purified. Their activity was tested using different concentrations of magnesium, calcium or zinc and different substrates. The optimal metal concentrations for catalysis were estimated. Both the single and double AtPreP1 variants were inactive against a 54 amino acid residue peptide corresponding to the ATPase F1β presequence. However, when the degradation was tested with five other shorter peptides, the results showed that the activity was substrate-specific and that it may depend on the substrate length. References: [1] Ståhl, A., Moberg, P., Ytterberg, J., Panfilov, O., Brockenhuus von Löwenhielm, H., Nilsson, F. and Glaser, E. (2002), Isolation and Identification of a Novel Mitochondrial Metalloprotease (PreP) that Degrades Targeting Presequences, J. Biol. Chem., 277 (44), 41931-41939. [2] Ståhl, A., Nilsson, S., Lundberg, P., Bhushan, B., Biverståhl, H., Moberg, P., Morisset, M., Vener, A., Mäler, L., Langel, U., and Glaser, E. (2005), Two Novel Targeting Peptide Degrading Proteases, PrePs, in Mitochondria and Chloroplasts, so Similar and Still Different, J. Mol. Biol., 349, 847–860. [3] Johnson, K.A., Bhushan, S., Ståhl, A., Hallberg, B.M., Frohn, A., Glaser, E. and Eneqvist, T. (2006), The closed structure of presequence protease PreP forms a unique 10 000 Å 3 chamber for proteolysis, EMBO J., 25 (9), 1977-1986. 56
Page 1 and 2:
IJUP08 First Meeting of Young Resea
Page 3 and 4:
Adrián Silva Dinis Cayolla Ribeiro
Page 5 and 6:
PERMEABILITY CHARACTERISTICS OF HIG
Page 7 and 8:
THE COURTYARD AND SIZA’S HOUSES__
Page 9 and 10:
INTRACELLULAR GENE REGULATION VIA E
Page 11 and 12:
COPPER(II)/CIPROFLOXACIN COMPLEXES:
Page 13 and 14:
INFLUENCE OF BEER ANTIOXIDATIVE PRO
Page 15 and 16:
INFORMATION SYSTEMS ABOUT MATERIALS
Page 17 and 18:
8:30 8:45 8:45 9:00 9:00 9:15 9:15
Page 19 and 20: INTERDISCIPLINARY ORAL SESSIONS - I
Page 21 and 22: Construction of a learning unit in
Page 23 and 24: Angiogenesis modulation by catechin
Page 25 and 26: Alfonso X’s General Estoria and t
Page 27 and 28: Alternative User Interfaces for Gam
Page 29 and 30: Treatment of Myasthenia gravis with
Page 31 and 32: Automatic Emotion Assessment throug
Page 33 and 34: Permeability Characteristics of Hig
Page 35 and 36: PARALLEL ORAL SESSIONS I - POS.I.1
Page 37 and 38: Antitumor and antifungal activities
Page 39 and 40: Synthesis and Determination of Phys
Page 41 and 42: Synergism between adenosine A2A and
Page 43 and 44: The knowledge-based economy as a he
Page 45 and 46: The Space of Intervals. The inner s
Page 47 and 48: Interactive Application for Spatial
Page 49 and 50: PARALLEL ORAL SESSIONS I - POS.I.3
Page 51 and 52: The dynamical state of galaxies: fr
Page 53 and 54: Anti-de Sitter space and the stabil
Page 55 and 56: Quantum Vacuum Energy in General Re
Page 57 and 58: Microplate high-throughput methodol
Page 59 and 60: Detection of genetically modified s
Page 61 and 62: Product Engineering - Application o
Page 63 and 64: PARALLEL ORAL SESSIONS II - POS.II.
Page 65 and 66: Organic living | Weekend house in C
Page 67 and 68: Casa Comum [common/collective/commu
Page 69: The silence and the sky: seclusion
Page 73 and 74: Anchoring of macrocycle compounds o
Page 75 and 76: Functional Insulin Quantification u
Page 77 and 78: PARALLEL ORAL SESSIONS III - POS.II
Page 79 and 80: MUC1 overexpression is associated w
Page 81 and 82: Prenylated and bromoalkylated xanth
Page 83 and 84: Impairment of retrograde signalling
Page 85 and 86: Contemporary condition: ‘new’ r
Page 87 and 88: Architecture and climate. Site geog
Page 89 and 90: Incorporation of organics/inorganic
Page 91 and 92: PARALLEL ORAL SESSIONS III - POS.II
Page 93 and 94: Exhibiting Architecture in Portugue
Page 95 and 96: Promenade to Corbusier L. Ribeiro d
Page 97 and 98: CONTACT POINTS_BETWEEN SWISS AND PO
Page 99 and 100: Levels of Butyltins in Sediment and
Page 101 and 102: Azo-dye Orange II degradation by Fe
Page 103 and 104: Isolation and characterisation of a
Page 105 and 106: PARALLEL ORAL SESSIONS IV - POS.IV.
Page 107 and 108: Natural resources course B. Magalh
Page 109 and 110: Desistência do tratamento de enure
Page 111 and 112: A troubled legacy. Changing strateg
Page 113 and 114: Screening of Phenolic compounds wit
Page 115 and 116: Determination of hypoxanthine in vi
Page 117 and 118: Comprehensibility of pictorial repr
Page 119 and 120: New Conditions for Greater Purifica
Page 121 and 122:
28 HPLC-DAD-MS/MS analysis of pheno
Page 123 and 124:
PHOTOCATALYTIC DEGRADATION OF CIPRO
Page 125 and 126:
209 Evaluation of genetic and chemi
Page 127 and 128:
Thermodynamic and Theoretical Study
Page 129 and 130:
Possible correlation between morbid
Page 131 and 132:
Determination of Cadmium Content in
Page 133 and 134:
Optimization of an enzymatic and ch
Page 135 and 136:
Low-cost materials as adsorbents fo
Page 137 and 138:
An expeditious method for determina
Page 139 and 140:
Effect of pyrophosphate on firefly
Page 141 and 142:
Touristic Topographies : transversa
Page 143 and 144:
Copper(II)/Ciprofloxacin complexes:
Page 145 and 146:
Feeling Architecture. The importanc
Page 147 and 148:
Authenticity evaluation and molecul
Page 149 and 150:
Computational Analisys of Cynara ca
Page 151 and 152:
Chromium remediation using zero val
Page 153 and 154:
Potentiometric determination of gen
Page 155 and 156:
DFT STUDY ON THE ABILITY OF CALIX[2
Page 157 and 158:
Steady state and lifetime chemical
Page 159 and 160:
Scopoletin, a natural coumarin isol
Page 161 and 162:
New approaches for estimating the p
Page 163 and 164:
Firing distance estimation through
Page 165 and 166:
Synthesis, structure elucidation an
Page 167 and 168:
Ultrastructural characterisation of
Page 169 and 170:
Physical exercise and stress hormon
Page 171 and 172:
PHOTOCATALYTIC DEGRADATION OF CIPRO
Page 173 and 174:
Geometry and Space Conception the i
Page 175 and 176:
Formation of heterocyclic aromatic
Page 177 and 178:
THE STUDY OF DE FÂNZERES-GONDOMAR
Page 179 and 180:
Resonant frequency shift / Hz Quart
Page 181 and 182:
Synthesis of xanthone derivatives b
Page 183 and 184:
MeO Synthesis, Structural Elucidati
Page 185 and 186:
Primary Hyperparathyroidism: the un
Page 187 and 188:
Effects of etoposide, doxorubicin a
Page 189 and 190:
Characterization of exotic wood spe
Page 191 and 192:
Interactions of sulindac and its me
Page 193 and 194:
The Japanese Influence in Modern We
Page 195 and 196:
Conduction block in isolated rat sc
Page 197 and 198:
Monitoring particulate matter using
Page 199 and 200:
Education and Poverty: an empowerme
Page 201 and 202:
Characterization of Urea-Formaldehy
Page 203 and 204:
Investigation of the Interactions b
Page 205 and 206:
Adenosine regulates differentiation
Page 207 and 208:
Pravastatin Quantification using Sq
Page 209 and 210:
Information Systems about Materials
Page 211 and 212:
Potential of Equisetum arvense hydr
Page 213 and 214:
Taxonomy from rural diffuse to urba
Page 215 and 216:
Study and design of precast reinfor
Page 217 and 218:
Influence of egg yolk and aromatizi
Page 219 and 220:
The metamorphosis of Aleixo towers
Page 221 and 222:
Screening for novel CDH1 inactivati
Page 223 and 224:
Genetic characterization by SSR of
Page 225 and 226:
PLANTAS DO EDIFÍCIO DA FACULDADE D
Page 227 and 228:
EDIFICIO D 2D19-1 D.T. 2D63-1 C1 2D
show all

IJUP08 - Universidade do Porto

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?