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Identification of putative odorant receptors from Epiphyas postvittana 104 Table 4.6 summarises the properties of the E. postvittana ORs, with the predicted coding region length available for each OR, its closest B. mori homologue based on pairwise distance matrix calculated in ClustalX and the method(s) that identified each OR. TMPRED was used for predicting the topology of the ORs for which more than 350 amino acids of the predicted coding regions were available. Of these, EpOR2, 32, 34 and 47 were predicted to have intracellular N-terminus (shaded in orange and purple on Figure 4.4). EpOR1 and EpOR6 share the most amino acid identity with BmOR1, a PR of B. mori. EpOR1 has been demonstrated to be a receptor for plant volatiles in Chapter 2; hence EpOR6 (shaded in yellow on Figure 4.4) is predicted to be a candidate PR of E. postvittana. Table 4.6: E. postvittana putative OR repertoire to date. The ORs were identified either by Sanger sequencing (S), 454 transcriptome sequencing (T) or low coverage whole genome sequencing (G). The B. mori homologues of the corresponding E. postvittana ORs are given, together with the percentage amino acid identity shared between the homologues. This data is based on the incomplete sequences of the putative E. postvittana ORs and can change as the full length sequences become available. Putative OR Length (amino acids) Closest B. mori OR identified with the available length % amino acid identity with B. mori ORs with the current sequence length Method EpOR1* 415 BmOR1 38 S, T, G EpOR2* 474 BmOR2 88 S, T, G EpOR3* 410 BmOR49J 65 S, T, G EpOR4 268 BmOR56 60 G EpOR5 304 BmOR68 22 G EpOR6 241 BmOR1 17 T, G EpOR7 308 BmOR4 26 T, G EpOR8 324 BmOR8 71 G EpOR9 225 BmOR4 14 G EpOR10 349 BmOR27 22 G EpOR11 113 BmOR26 19 G EpOR12 293 BmOR15 45 G EpOR13 271 BmOR13 52 T, G EpOR14 118 BmOR14 20 T, G EpOR15 319 BmOR13 20 T, G EpOR16 206 BmOR41 23 G EpOR17 243 BmOR49 60 G
Identification of putative odorant receptors from Epiphyas postvittana 105 EpOR18 312 BmOR30 37 G EpOR19 209 BmOR28 17 G EpOR20 131 BmOR14 37 T, G EpOR21 475 BmOR49 18 T, G EpOR22 84 BmOR6 32 T, G EpOR23 206 BmOR23 33 T, G EpOR24 114 BmOR24 28 T, G EpOR25 257 BmOR18 47 G EpOR26 188 BmOR26 22 G EpOR27 329 BmOR27 57 T, G EpOR28 170 BmOR28 15 G EpOR29 185 BmOR29 57 T, G EpOR30 257 BmOR30 28 T, G EpOR31 282 BmOR16 38 G EpOR32 359 BmOR32 41 T, G EpOR33 118 BmOR68 22 T, G EpOR34 395 BmOR33 30 T, G EpOR35 324 BmOR36 49 G EpOR36 152 BmOR61 18 T, G EpOR37 219 BmOR55 43 G EpOR38 162 BmOR38 55 G EpOR39 120 BmOR39 60 T, G EpOR40 113 BmOR16 31 G EpOR41 210 BmOR29 42 T, G EpOR42 293 BmOR42 33 T, G EpOR43 175 BmOR63 22 G EpOR44 211 BmOR44 29 G EpOR45 77 BmOR15 22 G EpOR46 221 BmOR47 17 G EpOR47 350 BmOR60 54 T, G EpOR48 97 BmOR52 21 T EpOR49 40 BmOR30 30 T EpOR50 70 BmOR46 20 T EpOR51 76 BmOR26 23 T EpOR52 59 BmOR64 27 T *These E. postvittana ORs were identified by Jordan et al. (2009), while all the other ORs have been identified in this study.
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Abstract Abstract Olfaction or the
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Acknowledgements Acknowledgements I
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Contents v Contents Abstract……
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Contents 4.2.5 Degenerate PCR .....
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List of Figures List of Figures Fig
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List of Tables List of Tables Table
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List of Abbreviations gDNA Genomic
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1.1 General overview 1 General Intr
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General Introduction 3 moth in loca
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General Introduction 5 Figure 1.1:
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General Introduction 7 1.5 Componen
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General Introduction 9 OBPs have al
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General Introduction 11 A model has
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General Introduction 15 Tanaka et a
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General Introduction 17 Table 1.1:
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General Introduction 21 et al., 200
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General Introduction 23 the attract
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General Introduction 25 leaves and
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General Introduction 29 Expression
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General Introduction 31 BmOr2 HvOr2
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General Introduction 33 for lepidop
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Functional characterisation of Epip
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Page 69 and 70: Functional characterisation of Epip
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Page 73 and 74: 3.1 Introduction 3 The roles of Epi
Page 75 and 76: The roles of Epiphyas postvittana G
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Page 97 and 98: Identification of putative odorant
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Page 149 and 150: Concluding Discussion 133 sequencin
Page 151 and 152: Appendix B 135 Buffered TB (Sambroo
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Page 161 and 162: Appendix E 145 VII continued Figure
Page 163 and 164: References 147 Ansebo, L., Ignell,
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References 155 Hrdy, I., F. Kuldova
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References 157 Kaissling, K. E. (19
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References 159 Krieger, J., Große-
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References 161 Lopez-Gomez, R. and
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References 163 Miura, N., Nakagawa,
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References 165 Pell, J. K., Macaula
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References 167 Røstelien, T., Stra
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References 169 Stowers, L. and Loga
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References 171 Turner, C. T., Davy,
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References 173 Vogt, R. G., Riddifo
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References 175 Widmayer, P., Heifet
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