Mechanisms of Olfaction in Insects - ResearchSpace@Auckland ...
Mechanisms of Olfaction in Insects - ResearchSpace@Auckland ...
Mechanisms of Olfaction in Insects - ResearchSpace@Auckland ...
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Conclud<strong>in</strong>g Discussion 133<br />
sequenc<strong>in</strong>g and assembly. The genome sequence dataset that we currently have has<br />
lots <strong>of</strong> gaps, as shown by the high number <strong>of</strong> scaffolds, approximately 843000<br />
scaffolds. This coverage is be<strong>in</strong>g improved by further sequenc<strong>in</strong>g <strong>of</strong> the genome.<br />
Another complementary approach to BAC sequenc<strong>in</strong>g is mate pair end sequenc<strong>in</strong>g<br />
(Illum<strong>in</strong>a, 2010), which will aga<strong>in</strong> give the ends <strong>of</strong> 5kb long fragments that can be<br />
used as a guide for assembl<strong>in</strong>g the shotgun sequenc<strong>in</strong>g fragments.<br />
The challenges faced with<strong>in</strong> the context <strong>of</strong> this thesis <strong>in</strong> identify<strong>in</strong>g new ORs from E.<br />
postvittana is reflected by the low levels <strong>of</strong> homology between the members <strong>of</strong> this<br />
group and the importance <strong>of</strong> high throughput sequenc<strong>in</strong>g technologies to such<br />
projects. With the identification and decod<strong>in</strong>g <strong>of</strong> <strong>in</strong>creas<strong>in</strong>g number <strong>of</strong> moth ORs,<br />
important questions about correlation <strong>of</strong> the phylogenetic relatedness <strong>of</strong> these prote<strong>in</strong>s<br />
with their ligands, as well as the occurrence and significance <strong>of</strong> multiple PRs <strong>in</strong> moths<br />
can be addressed.