Mechanisms of Olfaction in Insects - ResearchSpace@Auckland ...
Mechanisms of Olfaction in Insects - ResearchSpace@Auckland ...
Mechanisms of Olfaction in Insects - ResearchSpace@Auckland ...
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Functional characterisation <strong>of</strong> Epiphyas postvittana odorant receptor 1 48<br />
Table 2.1: Change <strong>in</strong> fluorescence at 10 -5 M <strong>of</strong> the compounds tested with EpOR1 <strong>in</strong><br />
the Sf9 cell assay. Each odorant was tested <strong>in</strong> triplicate from a s<strong>in</strong>gle transfection mix.<br />
Compound<br />
Group<br />
Compound Name ∆F a N c<br />
Monoterpene Limonene NR b 0<br />
Myrcene NR 0<br />
-p<strong>in</strong>ene NR 0<br />
L<strong>in</strong>alool NR 0<br />
Citronellol NR 0<br />
-terp<strong>in</strong>eol NR 0<br />
1,8 c<strong>in</strong>eole 0.43 ± 0.25 6<br />
Nerol 0.41 ± 0.14 8<br />
Geraniol 0.31 ± 0.03 8<br />
Citral 0.32 ± 0.02 7<br />
Geranial 0.36 ± 0.02 6<br />
Geranyl acetate 0.32 ± 0.02 6<br />
Sesquiterpene -farnesene 0.41 ± 0.12 7<br />
Caryophyllene NR 0<br />
Humulene NR 0<br />
Triterpene Squalene NR 0<br />
Alcohol Hexanol NR 0<br />
Nonanol NR 0<br />
Pentanol NR 0<br />
Octanol 0.25 ± 0.21 6<br />
Aldehyde Hexanal NR 0<br />
Ester Ethyl butyrate NR 0<br />
Ethyl octanoate NR 0<br />
Ethyl hexanoate NR 0<br />
Propyl propanoate NR 0<br />
Pentyl acetate 0.37 ± 0.31 8<br />
Hexyl acetate NR 0<br />
Dodecyl acetate NR 0<br />
Octyl acetate NR 0<br />
Propyl acetate NR 0<br />
(E)-11-tetradecenyl acetate NR 0<br />
Benzoate Eugenol NR 0<br />
Methyl salicylate 0.31 ± 0.03 6<br />
a. Average change <strong>in</strong> fluorescence ± SE <strong>of</strong> at least three cells.<br />
b. NR = no response as determ<strong>in</strong>ed by no change <strong>in</strong> fluorescence <strong>of</strong> the cells<br />
upon stimulation with the odorant <strong>in</strong> triplicates at 10 -5 M.<br />
c. N = number <strong>of</strong> respond<strong>in</strong>g cells. A m<strong>in</strong>imum <strong>of</strong> 300 cells were looked at to<br />
confirm that a compound did not elicit a calcium response <strong>in</strong> the cells.