Mechanisms of Olfaction in Insects - ResearchSpace@Auckland ...

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Appendix B 136 Figure B.1: Chromatogram showing the elution profile of His6-EpGOBP2 from HiTrap chelating HP column. The numbers in red indicate the elution fractions and His6-EpGOBP2 eluted as a single peak in fractions 16–21. Figure B.2: Elution profile of His6-EpGOBP2 on Q-sepharose HP column. The numbers in red indicate the elution fractions and His6-EpGOBP2 eluted in a single peak in fractions 25–31.
Appendix C 137 C. Appendix C – ClustalX multiple sequence alignment of moth PR clade MsOR1 ------MIFMDDPLSKSIKDPRDYRYMKLFRSTLRLIGSWPGR------DLKEEGATKYE BmOR-3 ------MIFVDDAVIG-IKDPREYRHLRVLRTSLRLLGAWPGH------YLGEETGSKYE HR6 -MNLRKFLFENEAVEG-INSPADYLYTRILRFNLDFIRTWPRK------ELGEPENLAFT HR16 -MGLRQFLFENEAVEG-INTASDYLYIKILRFTLVIVNSWPRK------EIGEPESPRLS HR14 MTGIRDFFFNYEAKDG-VTNPTEYPYMIMSRHLLTVITCWPKKPKEGLNARAKLRAKIWV HR15 MTGFRDFVFNYQPKDG-ITNPVDYPYLIIARYLLTFISMWPKKSVVYHSARAELKARIWL HR11 ------MHLAGNAVTG-ITGPMDYKYMKVLRFVLRIISGWPGK------ALGEKTLRIEG BmOR-5 ----MLLYYPNTQVKEKVNNVEEFTYIKFLKSFCKIMDFWP--------EREEKNSKTRI BmOR-7 ----MLLYHPNTQVEEKVNNVEEFTYMKFLKSFCKIMDFWP--------EREEKNSKTRI HR13 ----MKILSDGSDLEG-VEKVEDIFYINLARKSMWILDSWP--------KAFNASSKYRY BmOR-4 MFKIIKNIIVENDALKQVEKPQEFQYMKWVQYHLKYIDGWPNM------DMNKKNVSKIR BmOR-1 ----MLLSFKDDSRSPDIQKPQNFQYMKILRFNLKIICAWPE-------KQLNEIRSLGH EposOR1 ------------------MDVFNLKYMRMIRFTLRSIGAWPSHEFED--VPATKLTLLSS BmOR-6 -----------MKEEYYLQHPRTQLFYKVLAHVSTIESTIDLTWWG---YTFPKYVGWFY . MsOR1 IAPLYWVLVIKITCFVLTIIYLIENTNKLGFFEIGHVYITVFMTMITLSRSITLSLNPKY BmOR-3 CAPMFLLMFIKIACLYLTIVYLRNNADVLGFFELGHVYLTIFMTFVTLSRGFSLTWNPNY HR6 VFMQYFYLILNIVTVMGSTSYIVVRGSELSFIEAGLMYLIFLIGIVDTLTVVCLTFSEKF HR16 AFAKYFYLFLTVLAAIGSIAYVAVHNRELTFLETGHMYIVVLMSLVDVSRVATLTMSTTY HR14 IVQKIFHMSLCLLTTLGMAMYIGLHKKSMSFLELGHLYISLLMTVVIFSRITTLCLNPKY HR15 WVQKFYHLLLCAVAFFGGVLYITLHKKSMTFYELGHLYISLLMMACTFSRITTLCFNDEY HR11 MGHAYYNTILSLVYLALGIAYLKKNFHRFDFLELGQLYIVLLMNMLSTSRAFTLCLSQKY BmOR-5 FRLRYILVLQFCFTLVAGVLYLTNSVGKQTFYDLGHTIITVLMNVVSLSRLILR-CFKKY BmOR-7 FRLRYILVLQFCFTLVAGVLYLKNNFGKKTFYDLGHTIITVVMNVVSVSRLILR-CFKKY HR13 F----VLALN-VATLIGGAIYLRNNTGVLSSFELGHTYITVFMNCITCSRCLMI-LSKDY BmOR-4 FHKRHLLVVEQTITFLSQMFYIVKNYGKLSFFEIGHSYITALMTIVIFSRSVVT-ALGRY BmOR-1 SIHRVILPIQSVVCLACGILYIHFHFNEIPFFILASTFITVMMNLVTCSRTALVMLFERY EposOR1 YSYTCFLCIICCFGTIAQIAYLITNQGILGFIDLGQTYLTVLMCFVYIQRTMLP-LQTSY BmOR-6 HLQCNVVRLFGKCVVVSQILFIILNYQTIDKSVFIIAITITPLGALVGIKAESA-KAECY :: : : MsOR1 RRVMTKYITKMHLFYYK---DMSDIALKTHIRVHKLSHFFTMYLSTQVVLGTVTFNIVPM BmOR-3 HKVVKKFITEMHLLYFK---DNSEYAMKTHRRVHKISHFYTVFLKVQMIAGLTLFNVIPM HR6 RVLAKDFLTKTHLFYYK---DRSKHAMEIHKKIHLISHLFSLWILFQMLSGLSLFNIIPM HR16 REVARDFLTKIHLFYYK---DRSKHAMETHRAVHKISHLFTLWLVGQMLSGLSLFNLIPM HR14 RAVSTEFLTKIHLFYYK---DDSEFSMQIHKQVHKISHLFTLYLTGQMIAGLSLFNLTPM HR15 RVVAKDFVTKIHLFFYK---NRSDYSMQIHKKVHMISHVFTLYLSGQMMLGLFLFNVTPM HR11 REVAKIFIQKIHLFYFK---EKSDYAMKIHVIVHKISFISAVYLSVLLFIAAVMFNLIPM BmOR-5 DVVGQQFINKIHLYHYR---NDSEYAMKIHTVVHKISHNMTYIFSFCIIFGTVTFNLTPI BmOR-7 DVVGQQFINKIHLYHFR---NDSEYSMKTYKAVHKISNNMTYIFSFSIFVCVVTFNLNPV HR13 NHVMTLFVQKIHLFHHK---HKSDYAYLTHIFIHKISHFYTVYLLGLALNGLFLFNMIPF BmOR-4 RKIARYFVSSLHLYHYK---DISEYALQTHLLVHRLSHYYTVYLISLVVTGMLLFNITPL BmOR-1 LVLTGRFITVMHLFNFQ---KNSDYAYKLCTFVNRMSHFYTLYVLFSMFMGLGLFNLLPL EposOR1 QAEIIEFSTKFHLMYHK---NETEFAAKMHNKVKRICEIVTGIQHLQIYYVLVMYNIAPL BmOR-6 VNLMKNFMDKVHIHSIYRKNENNEFVKKKVIQIERVSRFTAYFLVILIAINCLSWMLKPT : *: . .. :. :. : : : * MsOR1 YNNYKVGRFEN-NILVNDSYELSIYFKTPTKFLSTLNGYIAITTFNWYSSYICSNFFCMF BmOR-3 YNNYRQGNYAS-DRPANITYDLSIYYET-FDILNTPNGYIFICVFNWFASYICCSFFCSF HR6 YSNLAAGKYRK-GGLQNSTFEHSLYYLYPFNTSTDITGYIIACILHWIISYLCSCWFCII HR16 YSNYAAGRYSG-DVSKNSTFEHSLYYSYPFDTSTDIRGYSIACVIHWVLSYLCSTWFCMF HR14 YNNFSAGKYKK-GGLKNSTFEHSLYYSYPFNASSDVGGYIVSNICDWIISYLCSTWFCTL HR15 YNNYSAGKYKS-GGLKNSTYEHSLYFSWPFNASTDMRGYIVSNILNWMLSYTCSSWFCVI HR11 YNNYSAGRYSSFDNLENTTYEQAISCLYPWNFETNFNGYLVATLSGWYGTMLCGSSVSMF BmOR-5 FNNIGSDAYKN-PRPDNVTLQQCVYYALPFDYTGNFKWYLLVAIFNVQKTFFCTSLFILF BmOR-7 FNNIGSGAYKN-PRPDNVTLQQCVYYALPFDYTGDFKWYMLVAIFNVQKTFFCTSLFILF HR13 YNCYSRGMFRD-VIPANATYDHAVFYSVPFDYTTKFKGYLAMTSFNVFISYTCTSYFCVV BmOR-4 YNNISSGVFNS-PRPENMTFQHAVYLGLPFDYTTDIKGYFVVFILNWHLSHIAASYFCTF BmOR-1 YNNYVSGAFSD-PYGPNVTFFHSVYFAFPFDYSHNFRGYIIMALFNSYVSVTCSIGLVMF
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Abstract Abstract Olfaction or the
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Acknowledgements Acknowledgements I
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Contents v Contents Abstract……
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Contents 4.2.5 Degenerate PCR .....
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List of Figures List of Figures Fig
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List of Tables List of Tables Table
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List of Abbreviations gDNA Genomic
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1.1 General overview 1 General Intr
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General Introduction 3 moth in loca
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General Introduction 5 Figure 1.1:
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General Introduction 7 1.5 Componen
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General Introduction 9 OBPs have al
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General Introduction 11 A model has
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General Introduction 15 Tanaka et a
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General Introduction 17 Table 1.1:
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General Introduction 21 et al., 200
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General Introduction 23 the attract
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General Introduction 25 leaves and
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General Introduction 29 Expression
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General Introduction 31 BmOr2 HvOr2
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General Introduction 33 for lepidop
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Functional characterisation of Epip
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3.1 Introduction 3 The roles of Epi
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The roles of Epiphyas postvittana G
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4.1 Introduction 4 Identification o
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Identification of putative odorant
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Identification of putative odorant
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Page 141 and 142: 5.1 Introduction 5 Concluding Discu
Page 143 and 144: Concluding Discussion 127 VOBA sett
Page 145 and 146: Concluding Discussion 129 redundant
Page 147 and 148: Concluding Discussion 131 against t
Page 149 and 150: Concluding Discussion 133 sequencin
Page 151: Appendix B 135 Buffered TB (Sambroo
Page 155 and 156: Appendix D 139 D. Appendix D - Solu
Page 157 and 158: Appendix E 141 I II
Page 159 and 160: Appendix E 143 V
Page 161 and 162: Appendix E 145 VII continued Figure
Page 163 and 164: References 147 Ansebo, L., Ignell,
Page 165 and 166: References 149 Butenandt, A., Beckm
Page 167 and 168: References 151 Fedurco, M., Romieu,
Page 169 and 170: References 153 Große-Wilde, E., St
Page 171 and 172: References 155 Hrdy, I., F. Kuldova
Page 173 and 174: References 157 Kaissling, K. E. (19
Page 175 and 176: References 159 Krieger, J., Große-
Page 177 and 178: References 161 Lopez-Gomez, R. and
Page 179 and 180: References 163 Miura, N., Nakagawa,
Page 181 and 182: References 165 Pell, J. K., Macaula
Page 183 and 184: References 167 Røstelien, T., Stra
Page 185 and 186: References 169 Stowers, L. and Loga
Page 187 and 188: References 171 Turner, C. T., Davy,
Page 189 and 190: References 173 Vogt, R. G., Riddifo
Page 191 and 192: References 175 Widmayer, P., Heifet
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