Mechanisms of Olfaction in Insects - ResearchSpace@Auckland ...

More documents

Recommendations

Info

General Introduction 30 this receptor is referred to as BmOR49 but in Tanaka et al. (2009), another OR has been annotated as BmOR49 so in this thesis, the BmOR49 of Anderson et al. (2009) will be referred to as BmOR49J], and tissue expression analysis showed it to be expressed in both male and female antennae at similar levels. Functional characterisation of EpOR3 by heterologous expression in Sf9 cells decoded its ligands to be members of terpenes, alcohols and esters at high concentrations, while at lower concentrations, the receptor bound citral, geraniol, geranial and geranyl acetate. Dose response curves showed EpOR3 has the highest affinity for the oviposition deterrent citral, with an EC50 value of 1.1 x 10 -13 M (Jordan et al., 2009), implicating a role of this receptor as an OR and not PR. Three ORs have so far been identified from E. postvittana; EpOR2 is the Drosophila OR83b homologue, EpOR3 is the receptor for general plant odorants while EpOR1 remains to be functionally characterised. Further analysis of these receptors and the identification of new receptors will lead to a better understanding of the olfactory mechanisms of this moth. This knowledge will aid in the development of pest control strategies targeting the olfactory system of the moth. Two GOBP members have also been identified; however this class of soluble proteins have very little functional data available, therefore an attempt will be made to deduce the role(s), if any, of this class of protein in odour perception in in vitro assay systems.
General Introduction 31 BmOr2 HvOr2 MsepOR2 DiOR2 PxOR2 EpOR2 0.1 100 * 80 HvOr15 HvOr14 MsepOR1 HvOr6 HvOr16 HvOr11 BmOr3 PxOR1 BmOr7 BmOr5 BmOr9 BmOr4 MsepOR3 HvOr13 DiOR3 BmOr1 DiOR1 EpOR1 PxOR3 PxOR4 Male-biased sex pheromone receptor clade 31 77 99 BmOr6 100 BmOr22 BmOr21 HvOr19 41 62 99 100 98 HvOr21 BmOr19 BmOr17 BmOr8 BmOr20 100 43 BmOr38 BmOr35 BmOr37 96 HvOr12 45 41 BmOr24 69 BmOr25 99 100 88 89 HvOr7 BmOr11 HvOr9 BmOr23 BmOr42 54 100 HvOr18 40 100 100 HvOr20 BmOr28 57 95 BmOr14 100 BmOr34 100 BmOr33 BmOr30 BmOr36 34 HvOr3 100 BmOR22J 100 BmOr15 99 BmOr12 30 73 100 100 HvOr8 BmOr13 EpOR3 * EpOR3 BmOR49 BmOR49J 100 100 BmOr48 BmOr46 Female-biased 100 BmOr47 BmOr45 receptor clade 100 BmOR19J 94 97 HvOr10 BmOr10 40 100 BmOr16 89 BmOR23J BmOr26 HvOr17 98 BmOr29 BmOr27 Or83b clade 98 99 48 100 60 25 100 Figure 1.8: Phylogenetic tree constructed from lepidopteran odorant receptors with the three E. postvittana ORs indicated by *. Adapted from Jordan et al. (2009) where the tree was originally constructed using the FITCH method from Jones-Thorton distances and the given bootstrap values calculated using 1000 replicates. 50 62 96 33 100 25 34 91 100 94 100 100 100 *
Page 1 and 2: http://researchspace.auckland.ac.nz
Page 3 and 4: Abstract Abstract Olfaction or the
Page 5 and 6: Acknowledgements Acknowledgements I
Page 7 and 8: Contents v Contents Abstract……
Page 9 and 10: Contents 4.2.5 Degenerate PCR .....
Page 11 and 12: List of Figures List of Figures Fig
Page 13 and 14: List of Tables List of Tables Table
Page 15 and 16: List of Abbreviations gDNA Genomic
Page 17 and 18: 1.1 General overview 1 General Intr
Page 19 and 20: General Introduction 3 moth in loca
Page 21 and 22: General Introduction 5 Figure 1.1:
Page 23 and 24: General Introduction 7 1.5 Componen
Page 25 and 26: General Introduction 9 OBPs have al
Page 27 and 28: General Introduction 11 A model has
Page 31 and 32: General Introduction 15 Tanaka et a
Page 33 and 34: General Introduction 17 Table 1.1:
Page 37 and 38: General Introduction 21 et al., 200
Page 39 and 40: General Introduction 23 the attract
Page 41 and 42: General Introduction 25 leaves and
Page 45: General Introduction 29 Expression
Page 49 and 50: General Introduction 33 for lepidop
Page 51 and 52: Functional characterisation of Epip
Page 73 and 74: 3.1 Introduction 3 The roles of Epi
Page 75 and 76: The roles of Epiphyas postvittana G
Page 95 and 96: 4.1 Introduction 4 Identification o
Page 97 and 98:
Identification of putative odorant
Page 99 and 100:
Page 101 and 102:
Page 103 and 104:
Page 105 and 106:
Page 107 and 108:
Page 109 and 110:
Microarray OR 454 Transcriptome OR
Page 111 and 112:
Page 113 and 114:
Page 115 and 116:
Page 117 and 118:
Page 119 and 120:
Page 121 and 122:
Page 123 and 124:
Page 125 and 126:
Page 127 and 128:
Page 129 and 130:
Page 131 and 132:
Page 133 and 134:
Page 135 and 136:
Page 137 and 138:
Page 139 and 140:
Page 141 and 142:
5.1 Introduction 5 Concluding Discu
Page 143 and 144:
Concluding Discussion 127 VOBA sett
Page 145 and 146:
Concluding Discussion 129 redundant
Page 147 and 148:
Concluding Discussion 131 against t
Page 149 and 150:
Concluding Discussion 133 sequencin
Page 151 and 152:
Appendix B 135 Buffered TB (Sambroo
Page 153 and 154:
Appendix C 137 C. Appendix C - Clus
Page 155 and 156:
Appendix D 139 D. Appendix D - Solu
Page 157 and 158:
Appendix E 141 I II
Page 159 and 160:
Appendix E 143 V
Page 161 and 162:
Appendix E 145 VII continued Figure
Page 163 and 164:
References 147 Ansebo, L., Ignell,
Page 165 and 166:
References 149 Butenandt, A., Beckm
Page 167 and 168:
References 151 Fedurco, M., Romieu,
Page 169 and 170:
References 153 Große-Wilde, E., St
Page 171 and 172:
References 155 Hrdy, I., F. Kuldova
Page 173 and 174:
References 157 Kaissling, K. E. (19
Page 175 and 176:
References 159 Krieger, J., Große-
Page 177 and 178:
References 161 Lopez-Gomez, R. and
Page 179 and 180:
References 163 Miura, N., Nakagawa,
Page 181 and 182:
References 165 Pell, J. K., Macaula
Page 183 and 184:
References 167 Røstelien, T., Stra
Page 185 and 186:
References 169 Stowers, L. and Loga
Page 187 and 188:
References 171 Turner, C. T., Davy,
Page 189 and 190:
References 173 Vogt, R. G., Riddifo
Page 191 and 192:
References 175 Widmayer, P., Heifet
show all

Mechanisms of Olfaction in Insects - ResearchSpace@Auckland ...

Create successful ePaper yourself

Delete template?

Save as template?