Mechanisms of Olfaction in Insects - ResearchSpace@Auckland ...

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The roles of Epiphyas postvittana GOBP2 in odour detection 72 0.35 0.30 0.25 0.20 ∆F 0.15 0.10 0.05 0.00 0.35 0.30 0.25 0.20 ∆F 0.15 0.10 0.05 0.00 A -12 -10 -8 -6 -4 [Geranyl Acetate] (Log M) -12 -10 -8 -6 -4 [Geranyl Acetate] (Log M) C 0.35 0.30 0.25 0.20 ∆F 0.15 0.10 0.05 0.00 0.35 0.30 0.25 0.20 ∆F 0.15 0.10 0.05 0.00 B -12 -10 -8 -6 -4 [Geranyl Acetate] (Log M) D -12 -10 -8 -6 -4 [Geranyl Acetate] (Log M) Figure 3.8: Dose response curves of EpOR1 to geranyl acetate in the concentration range of 10 -5 M to 10 -12 M in the absence of both DMSO and EpGOBP2 (A), with DMSO (B), or with EpGOBP2 (C), or with geranyl acetate solubilised in DMSO and the cells incubated with EpGOBP2 (D). Error bars represent the standard error of six to eight responding cells.
The roles of Epiphyas postvittana GOBP2 in odour detection 73 0.35 0.30 0.25 0.20 ∆F 0.15 0.10 0.05 0.00 0.35 0.30 0.25 0.20 ∆F 0.15 0.10 0.05 0.00 A -16 -14 -12 -10 -8 -6 -4 [Methyl Salicylate] (Log M) C -16 -14 -12 -10 -8 -6 -4 [Methyl Salicylate] (Log M) 0.35 0.30 0.25 0.20 ∆F 0.15 0.10 0.05 0.00 0.35 0.30 0.25 0.20 ∆F 0.15 0.10 0.05 0.00 B -16 -14 -12 -10 -8 -6 -4 [Methyl Salicylate] (Log M) D -16 -14 -12 -10 -8 -6 -4 [Methyl Salicylate] (Log M) Figure 3.9: Dose response curves of EpOR1 to methyl salicylate in the concentration range of 10 -5 M to 10 -16 M in the absence of both DMSO and EpGOBP2 (A), with DMSO (B), or with EpGOBP2 (C) or with methyl salicylate solubilised in DMSO and the cells incubated with EpGOBP2 (D). Error bars represent the standard error of six to eight responding cells.
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Abstract Abstract Olfaction or the
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Acknowledgements Acknowledgements I
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Contents v Contents Abstract……
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Contents 4.2.5 Degenerate PCR .....
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List of Figures List of Figures Fig
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List of Tables List of Tables Table
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List of Abbreviations gDNA Genomic
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1.1 General overview 1 General Intr
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General Introduction 3 moth in loca
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General Introduction 5 Figure 1.1:
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General Introduction 7 1.5 Componen
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General Introduction 9 OBPs have al
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General Introduction 11 A model has
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General Introduction 15 Tanaka et a
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General Introduction 17 Table 1.1:
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Page 37 and 38: General Introduction 21 et al., 200
Page 39 and 40: General Introduction 23 the attract
Page 41 and 42: General Introduction 25 leaves and
Page 43 and 44: General Introduction 27 Figure 1.7:
Page 45 and 46: General Introduction 29 Expression
Page 47 and 48: General Introduction 31 BmOr2 HvOr2
Page 49 and 50: General Introduction 33 for lepidop
Page 51 and 52: Functional characterisation of Epip
Page 73 and 74: 3.1 Introduction 3 The roles of Epi
Page 75 and 76: The roles of Epiphyas postvittana G
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Page 95 and 96: 4.1 Introduction 4 Identification o
Page 97 and 98: Identification of putative odorant
Page 109 and 110: Microarray OR 454 Transcriptome OR
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Identification of putative odorant
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5.1 Introduction 5 Concluding Discu
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Concluding Discussion 127 VOBA sett
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Concluding Discussion 129 redundant
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Concluding Discussion 131 against t
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Concluding Discussion 133 sequencin
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Appendix B 135 Buffered TB (Sambroo
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Appendix C 137 C. Appendix C - Clus
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Appendix D 139 D. Appendix D - Solu
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Appendix E 141 I II
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Appendix E 143 V
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Appendix E 145 VII continued Figure
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References 147 Ansebo, L., Ignell,
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References 149 Butenandt, A., Beckm
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References 151 Fedurco, M., Romieu,
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References 153 Große-Wilde, E., St
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References 155 Hrdy, I., F. Kuldova
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References 157 Kaissling, K. E. (19
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References 159 Krieger, J., Große-
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References 161 Lopez-Gomez, R. and
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References 163 Miura, N., Nakagawa,
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References 165 Pell, J. K., Macaula
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References 167 Røstelien, T., Stra
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References 169 Stowers, L. and Loga
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References 171 Turner, C. T., Davy,
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References 173 Vogt, R. G., Riddifo
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References 175 Widmayer, P., Heifet
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