Mechanisms of Olfaction in Insects - ResearchSpace@Auckland ...

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The roles of Epiphyas postvittana GOBP2 in odour detection 78 This research has demonstrated the role of EpGOBP2 as a solubilising agent for plant volatiles and shown that it can be used to reconstitute a Sf9 cell assay of EpOR1. This paves the way for further studies where the sensilla lymph can be replicated in tissue culture. The OR has been expressed in vitro, a role for the GOBP has been demonstrated, and an ODE can be added to this existing system to complete the sensilla lymph replica in tissue culture. Such a system would give further insights into the roles of other players in the moth olfactory system. Recent research has demonstrated that B. mori GOBP2 binds the sex pheromone components of B. mori, raising the question of a possible role of moth GOBPs in pheromone reception (Zhou et al., 2009; He et al., 2010). However, B. Mori GOBP2 is not expressed in pheromone responsive sensilla (Maida et al., 2005). EpGOBP2 can be further tested with the sex pheromone components of E. postvittana to confirm whether a pheromone binding function is unique to the Bombycidae or has been conserved in the Lepidoptera.
4.1 Introduction 4 Identification of putative odorant receptors from Epiphyas postvittana The significant role that several members of the order Lepidoptera play in agriculture as pests has sparked great interest in studying their olfactory system as this is essential for their detection and location of mating partners and host plants. ORs play a pivotal role in the recognition of different odorants and their activation results in neuronal signals which the insect‟s brain interprets (refer to section 1.5.2 for an overview). Decoding the receptor repertoire of the olfactory system will enable the identification of specific receptor–ligand combinations of functional importance to various species of moth. Several different approaches can be employed in the isolation of ORs and PRs. These approaches are discussed below. 4.1.1 Transcriptome sequencing – EST approach A library of cDNAs, synthesised from RNA typically from a specific tissue, is made by shotgun cloning of cDNAs into a plasmid vector, followed by transformation into suitable host, selection of clones, plasmid isolation and sequencing. A high proportion of DNA in higher organisms is non-coding, with only a small proportion involved in
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Abstract Abstract Olfaction or the
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Acknowledgements Acknowledgements I
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Contents v Contents Abstract……
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Contents 4.2.5 Degenerate PCR .....
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List of Figures List of Figures Fig
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List of Tables List of Tables Table
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List of Abbreviations gDNA Genomic
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1.1 General overview 1 General Intr
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General Introduction 3 moth in loca
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General Introduction 5 Figure 1.1:
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General Introduction 7 1.5 Componen
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General Introduction 9 OBPs have al
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General Introduction 11 A model has
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General Introduction 15 Tanaka et a
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General Introduction 17 Table 1.1:
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General Introduction 21 et al., 200
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General Introduction 23 the attract
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General Introduction 25 leaves and
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Page 45 and 46: General Introduction 29 Expression
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Page 51 and 52: Functional characterisation of Epip
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Concluding Discussion 129 redundant
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Concluding Discussion 131 against t
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Concluding Discussion 133 sequencin
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Appendix B 135 Buffered TB (Sambroo
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Appendix C 137 C. Appendix C - Clus
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Appendix D 139 D. Appendix D - Solu
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Appendix E 141 I II
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Appendix E 143 V
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Appendix E 145 VII continued Figure
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References 147 Ansebo, L., Ignell,
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References 149 Butenandt, A., Beckm
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References 151 Fedurco, M., Romieu,
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References 153 Große-Wilde, E., St
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References 155 Hrdy, I., F. Kuldova
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References 157 Kaissling, K. E. (19
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References 159 Krieger, J., Große-
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References 161 Lopez-Gomez, R. and
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References 163 Miura, N., Nakagawa,
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References 165 Pell, J. K., Macaula
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References 167 Røstelien, T., Stra
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References 169 Stowers, L. and Loga
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References 171 Turner, C. T., Davy,
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References 173 Vogt, R. G., Riddifo
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References 175 Widmayer, P., Heifet
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