Mechanisms of Olfaction in Insects - ResearchSpace@Auckland ...

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List of Abbreviations ΔF Change in fluorescence °C Degrees celsius 2D-GE Two dimensional gel electrophoresis ABP Antennal binding protein BAC Bacterial artificial chromosome BLAST Basic local alignment search tool bp Base pair(s) cAMP Cyclic adenosine monophosphate CCD Charge–coupled device cDNA Complementary deoxyribonucleic acid cGMP Cyclic guanosine monophosphate CMF Chloramphenicol CSP Chemosensory protein CT Cycle threshold C–terminus Carboxy–terminus DEPC Diethyl pyrocarbonate DIG Digoxigenin DMSO Dimethyl sulfoxide DNA Deoxyribonucleic acid dNTP Deoxynucleotide triphosphate dsDNA Double stranded deoxyribonucleic acid DSN Duplex-specific nuclease EAG Electroantennogram EC50 Half maximal effective concentration EDTA Ethylenediamine tetra-acetic acid EST Expressed sequence tag FID Flame ionisation detector G Gravitational constant GC Gas chromatography xii List of Abbreviations
List of Abbreviations gDNA Genomic deoxyribonucleic acid GOBP General odorant binding protein GPCR G protein–coupled receptor G protein Guanine nucleotide–binding proteins HCl Hydrochloric acid HEK293 Human embryonic kidney–293 IPM Integrated pest management IPTG Isopropyl-beta-D-1-thiogalactopyranoside kb kilo base kDa kilo Daltons LB Luria broth LDS Lithium dodecyl sulphate mRNA Messenger ribonucleic acid MWCO Molecular weight cut-off mtDNA Mitochondrial deoxyribonucleic acid NBT/BCIP Nitro blue tetrazolium chloride/5-bromo-4-chloro-3-indolyl phosphate NCBI National centre for biotechnology information N–terminus Amino–terminus OBP Odorant binding protein OD Optical density ODE Odorant degrading enzyme OR Olfactory receptor ORF Open reading frame ORN Olfactory receptor neuron OSN Olfactory sensory neuron PAGE Polyacrylamide electrophoresis PBAN Pheromone biosynthesis activating neuropeptide PBP Pheromone binding protein PBS Phosphate–buffered saline PCR Polymerase chain reaction PDE Pheromone degrading enzyme xiii
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Page 3 and 4: Abstract Abstract Olfaction or the
Page 5 and 6: Acknowledgements Acknowledgements I
Page 7 and 8: Contents v Contents Abstract……
Page 9 and 10: Contents 4.2.5 Degenerate PCR .....
Page 11 and 12: List of Figures List of Figures Fig
Page 13: List of Tables List of Tables Table
Page 17 and 18: 1.1 General overview 1 General Intr
Page 19 and 20: General Introduction 3 moth in loca
Page 21 and 22: General Introduction 5 Figure 1.1:
Page 23 and 24: General Introduction 7 1.5 Componen
Page 25 and 26: General Introduction 9 OBPs have al
Page 27 and 28: General Introduction 11 A model has
Page 31 and 32: General Introduction 15 Tanaka et a
Page 33 and 34: General Introduction 17 Table 1.1:
Page 37 and 38: General Introduction 21 et al., 200
Page 39 and 40: General Introduction 23 the attract
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Page 45 and 46: General Introduction 29 Expression
Page 47 and 48: General Introduction 31 BmOr2 HvOr2
Page 49 and 50: General Introduction 33 for lepidop
Page 51 and 52: Functional characterisation of Epip
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Functional characterisation of Epip
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3.1 Introduction 3 The roles of Epi
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The roles of Epiphyas postvittana G
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4.1 Introduction 4 Identification o
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Identification of putative odorant
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Microarray OR 454 Transcriptome OR
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5.1 Introduction 5 Concluding Discu
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Concluding Discussion 127 VOBA sett
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Concluding Discussion 129 redundant
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Concluding Discussion 131 against t
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Concluding Discussion 133 sequencin
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Appendix B 135 Buffered TB (Sambroo
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Appendix C 137 C. Appendix C - Clus
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Appendix D 139 D. Appendix D - Solu
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Appendix E 141 I II
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Appendix E 143 V
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Appendix E 145 VII continued Figure
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References 147 Ansebo, L., Ignell,
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References 149 Butenandt, A., Beckm
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References 151 Fedurco, M., Romieu,
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References 153 Große-Wilde, E., St
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References 155 Hrdy, I., F. Kuldova
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References 157 Kaissling, K. E. (19
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References 159 Krieger, J., Große-
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References 161 Lopez-Gomez, R. and
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References 163 Miura, N., Nakagawa,
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References 165 Pell, J. K., Macaula
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References 167 Røstelien, T., Stra
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References 169 Stowers, L. and Loga
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References 171 Turner, C. T., Davy,
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References 173 Vogt, R. G., Riddifo
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References 175 Widmayer, P., Heifet
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