Mechanisms of Olfaction in Insects - ResearchSpace@Auckland ...
Mechanisms of Olfaction in Insects - ResearchSpace@Auckland ...
Mechanisms of Olfaction in Insects - ResearchSpace@Auckland ...
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
The roles <strong>of</strong> Epiphyas postvittana GOBP2 <strong>in</strong> odour detection 78<br />
This research has demonstrated the role <strong>of</strong> EpGOBP2 as a solubilis<strong>in</strong>g agent for plant<br />
volatiles and shown that it can be used to reconstitute a Sf9 cell assay <strong>of</strong> EpOR1. This<br />
paves the way for further studies where the sensilla lymph can be replicated <strong>in</strong> tissue<br />
culture. The OR has been expressed <strong>in</strong> vitro, a role for the GOBP has been<br />
demonstrated, and an ODE can be added to this exist<strong>in</strong>g system to complete the<br />
sensilla lymph replica <strong>in</strong> tissue culture. Such a system would give further <strong>in</strong>sights <strong>in</strong>to<br />
the roles <strong>of</strong> other players <strong>in</strong> the moth olfactory system. Recent research has<br />
demonstrated that B. mori GOBP2 b<strong>in</strong>ds the sex pheromone components <strong>of</strong> B. mori,<br />
rais<strong>in</strong>g the question <strong>of</strong> a possible role <strong>of</strong> moth GOBPs <strong>in</strong> pheromone reception (Zhou<br />
et al., 2009; He et al., 2010). However, B. Mori GOBP2 is not expressed <strong>in</strong><br />
pheromone responsive sensilla (Maida et al., 2005). EpGOBP2 can be further tested<br />
with the sex pheromone components <strong>of</strong> E. postvittana to confirm whether a<br />
pheromone b<strong>in</strong>d<strong>in</strong>g function is unique to the Bombycidae or has been conserved <strong>in</strong> the<br />
Lepidoptera.