Mechanisms of Olfaction in Insects - ResearchSpace@Auckland ...

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Identification of putative odorant receptors from Epiphyas postvittana 116 TMMOD DAS TMPRED SPLIT TOPPRED HMMTOP TMHMM N L1 L2 L3 L4 L5 L6 C Intracellular I II III IV V VI VII 0 50 100 150 200 250 300 350 400 Figure 4.8: Schematic representation of the consensus of the predicted TM regions from seven TM prediction programs. The black blocks represent the predicted TM regions by the respective program on the left, and are drawn to scale, corresponding to the predicted coding region of the gene, as indicated by the ruler at the bottom. Number I to VII are the seven consensus TM domain regions, N- and C- terminus are indicated at the ends and the intercellular loops indicated by L1 to L6. The transmembrane prediction program, TMHMM2.0 predicted five TM domains (TM1, 2, 4, 5 and 6) for EpOR34 with high probability, TM3 with low probability while TM 7 was not predicted at all. These results were compared with the TM prediction from TMMOD program. This predicted TM 1-6 with high probability Residue while TM7 was also predicted albeit with lower probability.
Identification of putative odorant receptors from Epiphyas postvittana 117 I II III IV V VI VII Figure 4.9: Transmembrane domain prediction of EpOR34 with TMHMM 2.0 algorithms. The y-axis shows the probability of an amino acid to be part of a TM domain. The numbered red shaded areas indicate predicted TM domains. I II III IV V VI VII Figure 4.10: Transmembrane domain prediction of EpOR34 with TMMOD algorithms. The y-axis shows the probability of an amino acid to be part of a TM domain. The numbered red shaded areas indicate predicted TM domains.
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Abstract Abstract Olfaction or the
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Acknowledgements Acknowledgements I
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Contents v Contents Abstract……
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Contents 4.2.5 Degenerate PCR .....
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List of Figures List of Figures Fig
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List of Tables List of Tables Table
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List of Abbreviations gDNA Genomic
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1.1 General overview 1 General Intr
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General Introduction 3 moth in loca
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General Introduction 5 Figure 1.1:
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General Introduction 7 1.5 Componen
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General Introduction 9 OBPs have al
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General Introduction 11 A model has
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General Introduction 15 Tanaka et a
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General Introduction 17 Table 1.1:
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General Introduction 21 et al., 200
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General Introduction 23 the attract
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General Introduction 25 leaves and
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General Introduction 29 Expression
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General Introduction 31 BmOr2 HvOr2
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General Introduction 33 for lepidop
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Functional characterisation of Epip
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3.1 Introduction 3 The roles of Epi
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The roles of Epiphyas postvittana G
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Page 151 and 152: Appendix B 135 Buffered TB (Sambroo
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Page 161 and 162: Appendix E 145 VII continued Figure
Page 163 and 164: References 147 Ansebo, L., Ignell,
Page 165 and 166: References 149 Butenandt, A., Beckm
Page 167 and 168: References 151 Fedurco, M., Romieu,
Page 169 and 170: References 153 Große-Wilde, E., St
Page 171 and 172: References 155 Hrdy, I., F. Kuldova
Page 173 and 174: References 157 Kaissling, K. E. (19
Page 175 and 176: References 159 Krieger, J., Große-
Page 177 and 178: References 161 Lopez-Gomez, R. and
Page 179 and 180: References 163 Miura, N., Nakagawa,
Page 181 and 182: References 165 Pell, J. K., Macaula
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References 167 Røstelien, T., Stra
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References 169 Stowers, L. and Loga
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References 171 Turner, C. T., Davy,
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References 173 Vogt, R. G., Riddifo
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References 175 Widmayer, P., Heifet
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