Gamma Rays and CarbonIon-Beams Irradiation for Mutation ...

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Table 11. Infection index (II-%), disease development period (DDP-days) per inoculated leaf and leaf-discs necrotic area (LDNA-%) in ion-beams irradiated ‘Williams’ plantlets. Cultivar/code No. Black Sigatoka-infection index (%)/evaluation 123 DDP/inoculated leaf No. Williams 16-Jun-06 27-Jun-06 10-Jul-06 24-Jul-06 1 2 3 Leafdiscs necrotic area (%) W 0 II 45 - 25.0 50.0 40.5 54 49.6 48.0 57.20 W 0 II 39 27.1 42.6 50.0 40.5 46.8 51 34.6 - W0.5 II 30 22.2 21.4 42.9 66.7 44 48.2 46.0 60.83 W0.5 II 46 27.8 29.2 40.0 76.7 46.8 44 38.8 76.36 W0.5 I 8 25.0 33.3 71.4 77.8 58.4 45.4 44.0 59.23 W 1 I 59 26.2 43.8 61.9 61.7 54.6 45.4 48.0 48.95 W 1 II 31 14.3 29.2 45.2 40.5 53.2 48.2 46.0 33.12 W 1 II 19 16.7 20.4 46.3 46.7 58.4 48.2 52.0 38.86 W 1 II 25 8.3 27.8 60.4 61.1 45 49.6 38.0 96.53 W 1 II 75 14.8 29.2 48.1 78.6 54.4 51 41.2 67.63 W 1 I 3 16.7 25.0 40.5 70.0 50.4 51 38.0 53.05 W 1 II 148 24.1 22.9 30.6 33.3 52.6 55 38.0 28.84 W 2 II 35 31.5 31.3 57.1 62.5 48.4 52 38.0 51.68 W 2 II 37 13.0 64.6 83.3 80.0 43 44 35.2 - W 2 I 62 16.7 25.9 61.1 81.3 61.4 46.2 35.2 77.32 W 2 I 61 13.0 33.3 46.7 73.3 43 44 31.0 66.13 W 2 I 52 24.1 27.8 46.3 52.4 59 49.6 38.8 57.05 W 2 I 7 22.9 28.6 45.2 46.7 43 51 42.0 56.27 W 2 II 29 33.3 28.6 64.3 78.6 59 46.8 38.8 59.82 W 4 II 25 20.8 40.7 33.3 42.9 48 55 38.0 36.04 W 4 II 7 20.4 33.3 60.0 79.2 47.6 49.6 38.0 55.19 W 4 II 25 - 36.7 43.8 46.7 52.2 54.2 48.0 40.55 W 8 II 71 22.2 20.8 68.2 83.3 47 49.6 33.8 46.97 W 8 II 13 12.5 27.1 43.8 40.5 57.6 49.6 46.0 33.66 W 16 II 11 14.8 29.6 62.5 70.0 54.4 48.2 36.6 50.88 W 16 II 74 14.6 16.7 35.0 46.3 56.8 53.4 52.0 46.15 W 16 I 45 25.0 33.3 47.6 30.6 45.8 49.6 38.0 37.63 W 16 I 50 27.1 19.0 53.3 80.0 46 44 32.4 57.60 W 16 I 14 20.8 22.9 55.6 90.0 50.4 44 45.2 38.74 W 32 I 82 28.3 16.7 56.3 72.2 56.8 44 37.4 94.20 W 32 II 20 16.7 37.5 47.6 69.4 43 49.6 38.0 78.25 W 64 I 100 27.1 31.3 56.3 86.1 56.2 44 36.6 70.63 W 64 I 37 20.4 38.9 45.2 38.1 55.4 48.2 38.0 34.88 W 64 I 97 - 22.2 54.8 60.0 43 44 38.0 - W 128 II 27 16.7 37.0 52.8 73.3 49.4 46.8 38.8 96.12 W 128 II 44 25.0 20.4 41.7 42.6 50 48.2 41.2 34.81 W 128 I 55 18.8 25.9 45.8 41.7 58.4 45.4 49.2 - W 128 II 29 18.5 27.8 59.5 66.7 57.6 51 38.0 62.15 W 128 I 67 13.9 18.8 39.6 38.9 60.6 48.2 48.0 36.02 W 128 II 1 23.8 22.2 52.1 73.8 58.4 46.8 38.0 43.96
Table 12. DDP-days, II-% and LDNA-% of selected candidates in ‘Williams’ and ‘Cavendish Enano’. Candidate code No. DDP-days II-% LDNA-% W 1 II 19 52.9 32.5 38.9 W 1 II 31 49.1 32.3 33.1 W 1 II 148 48.5 27.7 28.8 W 8 II 13 51.1 31.0 33.7 W 16 II 74 54.1 28.1 46.2 W 128 I 67 52.3 27.8 36.0 CE 4 II 30 53.6 31.0 40.7 CE 64 I 5 53.5 30.4 46.9 Letter in code number means W = ‘Williams’ and CE = ‘Cavendish Enano’. Table 13. Factor of effectiveness and putative mutations by carbon ion- beams observed in ‘Cavendish Enano’. Mutagen No. of total plants No. plants with variation 124 Characteristics Gy dose Carbon ion beam 42 2 Good response against to black Sigatoka Carbon ion beam 85 1 Fast growth plantlet inducing mutation (n) 4 (1) 64 (1) Factor of effectiveness 2.38 2.38 4 (1) 1.17 Carbon ion beam 29 5 Hexaploid 4 (5) 17.24 (%)
Page 1 and 2:
Gamma Rays and Carbon Ion-Beams Irr
Page 3 and 4:
Table of contents Chapter 1 1.1. In
Page 5 and 6:
4.2.1.1. Cultivars of banana……
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and ‘Valery’, which are exporte
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‘Cavendish Enano’, ‘Williams
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Although it is now found only in Au
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varieties bred at the Jamaican prog
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such as ‘Paka’ (AA) and ‘Pisa
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Resistance (PR). In connection with
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large number of improved mutant cro
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which migrated faster (Rf = 0.44) t
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is juglone (5 hydroxy-1,4-napthoqui
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experiments involving various biolo
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Tanaka (1999) reported novel genes
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2. 1. Materials and Methods Chapter
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2. 1. 1. 4. FHIA-01 ‘FHIA-01’(M
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(Gamma room)” and “Carbon ion-b
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esulting from “Gamma Room” expe
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traits as female sterility and part
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3. 2. 1. 3. Chronic irradiation ( 1
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Gy. ‘Williams’ and ‘Orito’
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a diploid clone (genomic constituti
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‘Williams’ (93%), the necrotic
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etween the irradiation doses and th
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moment they are at the development
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A B C D Fig. 2. Cultivars of banana
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Weight of plantlets (g) Height of p
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Control 50 Gy 100 Gy 150 Gy Fig. 6.
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Control 50 Gy 100 Gy 150 Gy 200 Gy
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Survival rate (%) for LD50 Survival
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A B C D E F Fig. 12. A plant from
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B D AA E Fig 14. Banana meristems a
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A B C Orito Orito Orito Cavendish E
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No. plants No. plants No. plants No
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Circunference (cm) Plant height (cm
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Table 1. Differences of the plantle
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Table 3. Differences of the surviva
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Table 5. Selected materials reporti
Page 77 and 78: Table 8. Factor of effectiveness an
Page 79 and 80: deletion rather than point mutation
Page 81 and 82: were recorded. From the results, th
Page 83 and 84: 4. 2. 2. Assessment of banana plant
Page 85 and 86: placement among the plants (Fig. 30
Page 87 and 88: hours, photographs of the leaf-disc
Page 89 and 90: ‘Williams’ (B) was obtained by
Page 91 and 92: class limits values are clearly sep
Page 93 and 94: values in ‘Cavendish Enano’ ran
Page 95 and 96: Only a partial resistance was obser
Page 97 and 98: Sigatoka but also fruit quality, po
Page 99 and 100: A C Fig. 23. Banana plantlets packa
Page 101 and 102: A C Fig. 25. Acclimatization of the
Page 103 and 104: A C Fig. 27. Survival plantlets for
Page 105 and 106: A C Fig. 29. Mycosphaerella fijiens
Page 107 and 108: A C Fig. 31. Labor during transplan
Page 109 and 110: B A Fig. 33. Establishment of the j
Page 111 and 112: A B Fig. 35. Regenerated plantlets
Page 113 and 114: Survival rate (%) for LD50 Survival
Page 115 and 116: 0 0.5 1 2 4 Fig 39. Banana plantlet
Page 117 and 118: A B C Fig. 41. Regenerated explants
Page 119 and 120: Range of optimum doses (Gy) 16 15 1
Page 121 and 122: Percent of plants Percent of plants
Page 123 and 124: LDNA-% LDNA-% DDP-days 100 80 60 40
Page 125 and 126: Fig. 49. Hexaploid cells in ‘Cave
Page 127: Table 10. Infection index (II-%), d
Page 131 and 132: Chapter 5 General Discussion Gamma
Page 133 and 134: FHIA-01 were not able to compare du
Page 135 and 136: ‘W 1 II 31’. In the case of ‘
Page 137 and 138: Summary Both Gamma rays and Carbon
Page 139 and 140: A ‘Cavendish Enano’ plant with
Page 141 and 142: I extend my gratitude also to Dr. K
Page 143 and 144: Cohan, J, P., C. Abadie, K. Tomekp
Page 145 and 146: Hase, Y., M. Yamaguchi and A. Tanak
Page 147 and 148: Molina, G. C. and J. P. Krausz (198
Page 149 and 150: Roux, N. S. (2001). Mutation induct
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