Gamma Rays and CarbonIon-Beams Irradiation for Mutation ...

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ACKNOWLEDGEMENT My entire gratitude to my academic adviser, Dr. Hiroshi Gemma, Professor of the Institute of Agriculture and Forestry, University of Tsukuba for his essential supervision, guidance, availability, assistance and efficient help during my research; to Dr. Yoritoshi Okuno, Professor of the Institute of Agriculture and Forestry, University of Tsukuba for his critical review and commentary on the manuscript and Dr. Toshiya Yamamoto, Professor of the University of Tsukuba, Doctor Program of Advanced Agriculture Technology and Sciences for his valuable corrections and suggestions. I would like to thanks to Dr. Sumiko Sugaya, Assistant Professor of the Laboratory of Pomology of the Institute of Agriculture and Forest, University of Tsukuba for her outstanding guidance and support of my research and Dr. Yoshihiko Sekozawa, Laboratory of Pomology of the Institute of Agriculture and Forest, University of Tsukuba for his support of my research. My appreciation to Dr. Shigeki Nagatomi, former Head of the National Institute of Radiation Breeding, for his technical advice and assistance on the establishment of the experiments on Gamma ray irradiation, providing necessary facilities, which made this research possible. Thankfulness to Dr. Atsushi Tanaka, Head of the Institute of Takasaki Ion Accelerators for Advanced Radiation Application (TIARA), Japan Atomic Energy Agency (JAEA), Japan, for is collaboration providing necessary facilities to make this research possible and Dr. Yoshihiro Hase, Researcher of the (TIARA-JAEA), Japan, for his valuable orientation on irradiation for better conduction of this research 135
I extend my gratitude also to Dr. Kazuo Watanabe, Professor of the University of Tsukuba, Doctor Program of Bio-Industrial Sciences for his technical assistance during flow cytometer and viruses analysis. Thanks to M. Sc. Ingnacio Sotomayor Herrera, Director of the Estación Experimental Tropical Pichilingue (EETP), Instituto Nacional Autónomo de Investigaciones Agropecuarias (INIAP), for his disinterested collaboration providing facilities for the normal conductance of the field experiments, to M. Sc. Iván Garzón, Head of the Laboratory of Biotechnology, Estación Experimental Tropical Pichilingue (EETP), Instituto Nacional Autónomo de Investigaciones Agropecuarias (INIAP), for his support for evaluations of the banana materials at nursery and field conditions. I am appreciative to Dr. Carmen Suarez, M. Sc. Danilo Vera and Egdo. Roy Vera, Laboratory of Phytopathology, Estación Experimental Tropical Pichilingue (EETP), Instituto Nacional Autónomo de Investigaciones Agropecuarias (INIAP), providing M. fijiensis colonies for a better conductance of this project and Ing. Mariuxi Cedeño, Tcnga. Behtty Castillo, Mr. Roberto Loza, Laboratory of Biotechnology (EETP-INIAP), for their valuable collaboration for evaluations at field conditions. I am grateful for Ms. Danilda Duran Hufana for her unconditional help and editing of the manuscript and M. Sc. Luis Duicela for his support with the statistical analysis. I wish to thank the Ministry of Education, Science and Culture of Japan for the scholarship allowing me to purse my Doctoral degree at the University of Tsukuba, Japan. Thanks also to all those who have contributed from the start and completion of this study and whose names are not mentioned. 136
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Gamma Rays and Carbon Ion-Beams Irr
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Table of contents Chapter 1 1.1. In
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4.2.1.1. Cultivars of banana……
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and ‘Valery’, which are exporte
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‘Cavendish Enano’, ‘Williams
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Although it is now found only in Au
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varieties bred at the Jamaican prog
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such as ‘Paka’ (AA) and ‘Pisa
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Resistance (PR). In connection with
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large number of improved mutant cro
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which migrated faster (Rf = 0.44) t
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is juglone (5 hydroxy-1,4-napthoqui
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experiments involving various biolo
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Tanaka (1999) reported novel genes
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2. 1. Materials and Methods Chapter
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2. 1. 1. 4. FHIA-01 ‘FHIA-01’(M
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(Gamma room)” and “Carbon ion-b
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esulting from “Gamma Room” expe
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traits as female sterility and part
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3. 2. 1. 3. Chronic irradiation ( 1
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Gy. ‘Williams’ and ‘Orito’
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a diploid clone (genomic constituti
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‘Williams’ (93%), the necrotic
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etween the irradiation doses and th
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moment they are at the development
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A B C D Fig. 2. Cultivars of banana
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Weight of plantlets (g) Height of p
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Control 50 Gy 100 Gy 150 Gy Fig. 6.
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Control 50 Gy 100 Gy 150 Gy 200 Gy
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Survival rate (%) for LD50 Survival
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A B C D E F Fig. 12. A plant from
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B D AA E Fig 14. Banana meristems a
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A B C Orito Orito Orito Cavendish E
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No. plants No. plants No. plants No
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Circunference (cm) Plant height (cm
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Table 1. Differences of the plantle
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Table 3. Differences of the surviva
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Table 5. Selected materials reporti
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Table 8. Factor of effectiveness an
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deletion rather than point mutation
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were recorded. From the results, th
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4. 2. 2. Assessment of banana plant
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placement among the plants (Fig. 30
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hours, photographs of the leaf-disc
Page 89 and 90: ‘Williams’ (B) was obtained by
Page 91 and 92: class limits values are clearly sep
Page 93 and 94: values in ‘Cavendish Enano’ ran
Page 95 and 96: Only a partial resistance was obser
Page 97 and 98: Sigatoka but also fruit quality, po
Page 99 and 100: A C Fig. 23. Banana plantlets packa
Page 101 and 102: A C Fig. 25. Acclimatization of the
Page 103 and 104: A C Fig. 27. Survival plantlets for
Page 105 and 106: A C Fig. 29. Mycosphaerella fijiens
Page 107 and 108: A C Fig. 31. Labor during transplan
Page 109 and 110: B A Fig. 33. Establishment of the j
Page 111 and 112: A B Fig. 35. Regenerated plantlets
Page 113 and 114: Survival rate (%) for LD50 Survival
Page 115 and 116: 0 0.5 1 2 4 Fig 39. Banana plantlet
Page 117 and 118: A B C Fig. 41. Regenerated explants
Page 119 and 120: Range of optimum doses (Gy) 16 15 1
Page 121 and 122: Percent of plants Percent of plants
Page 123 and 124: LDNA-% LDNA-% DDP-days 100 80 60 40
Page 125 and 126: Fig. 49. Hexaploid cells in ‘Cave
Page 127 and 128: Table 10. Infection index (II-%), d
Page 129 and 130: Table 12. DDP-days, II-% and LDNA-%
Page 131 and 132: Chapter 5 General Discussion Gamma
Page 133 and 134: FHIA-01 were not able to compare du
Page 135 and 136: ‘W 1 II 31’. In the case of ‘
Page 137 and 138: Summary Both Gamma rays and Carbon
Page 139: A ‘Cavendish Enano’ plant with
Page 143 and 144: Cohan, J, P., C. Abadie, K. Tomekp
Page 145 and 146: Hase, Y., M. Yamaguchi and A. Tanak
Page 147 and 148: Molina, G. C. and J. P. Krausz (198
Page 149 and 150: Roux, N. S. (2001). Mutation induct
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