Gamma Rays and CarbonIon-Beams Irradiation for Mutation ...

generation (mother plant and sucker) showed the same characteristics, this
characteristic is heritable to the subsequent generations. Unfortunately, bunch
characteristics and fruit quality were not able to be compared, but future
research could be conducted for leaf shape in relation to production and fruit
quality. On the other hand, Carbon ion-beams irradiated plants showed
hexaploids cells detected by flow cytometer (five plants in ‘C. Enano’ and one
in ‘Williams’), signifying that chromosome duplication occurred.
On the topic of Gamma rays irradiated plants in “Gamma
Greenhouse”, visible variation of the leaves such as abnormal, double, long,
rudimentary, spindled and yellow spotted leaf were shown, suggesting that the
effect of the long-term chronic irradiation acted directly in the active cell
division at meristem level, resulting in severe damage or even death of the
meristems.
A screening method using juglone toxin was adapted using the leaf
disc from young banana irradiated plantlets. Leaf disc necrotic area (LDNA-
%) was determined selecting the less affected plants form the irradiated
population (Gamma rays). Twenty plants were selected from the ‘Orito’ lot,
eight in ‘Williams’ and five in ‘Cavendish Enano’.
Applying a lineal regression to the combinations of the disease
development period (DDP-days), infection index (II-%) and LDNA-% on
plants from ion beam experiment, six plants of ‘Williams’ (code number: ‘W
16 II 74’, ‘W 128 I 67’, ‘W 1 II 148’, ‘W 8 II 13’, ‘W 1 II 19’ and ‘W 1 II
31’) and two plants of ‘Cavendish Enano’ (code numbers: ‘CE 4 II 30’ and
‘CE 64 I 5’) were selected as possible candidates with better response against
to black Sigatoka.
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