Gamma Rays and CarbonIon-Beams Irradiation for Mutation ...
Gamma Rays and CarbonIon-Beams Irradiation for Mutation ...
Gamma Rays and CarbonIon-Beams Irradiation for Mutation ...
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Concerning to the weight <strong>and</strong> height of plantlets on the ‘Cavendish<br />
Enano’, ‘Williams’, ‘FHIA-01’ <strong>and</strong> ‘Orito’ using <strong>Gamma</strong> rays <strong>and</strong> ‘Cavendish<br />
Enano’ <strong>and</strong> ‘Williams’ with the first irradiation of Carbon ion beams up to 16<br />
Gy, the tendency was to decrease weight <strong>and</strong> height when the irradiation was<br />
increased. When second Carbon ion beam irradiation was applied, the explants<br />
behave different in cluster’s height <strong>and</strong> weight. ‘Williams’ which was the one<br />
irradiated twice by Carbon ion-beams tend to remind similar in cluster’s<br />
height <strong>and</strong> weight up to 8 Gy. The same pattern showed ‘Orito’ <strong>and</strong> ‘FHIA-<br />
01’.<br />
According to the results obtained by <strong>Gamma</strong> rays in the “<strong>Gamma</strong><br />
room”, the survival rate of ‘FHIA-01’ was higher than the other cultivars <strong>and</strong><br />
was even able to sustain dosages of up to 500 Gy but with diminishing<br />
survival rate. This result suggests that ‘FHIA-01’ can be used in future trials<br />
utilizing doses between 150 <strong>and</strong> 500 Gy. In addition, The flow cytometer<br />
analysis reported difference of relative DNA content only in ‘FHIA-01’ when<br />
200 <strong>and</strong> 300 Gy were applied, suggesting that DNA deletion occurred, being<br />
definitely a promising cultivar with high sensitivity response to Gy exposure<br />
with a high chance of improving its quality by mutation induction. Seventeen<br />
plant of ‘FHIA-01’ were selected with diminished relative DNA content <strong>and</strong><br />
growth at greenhouse conditions. An interesting sigmoid drooping leaf plant<br />
mutation of ‘FHIA-01’ have been occurred. The characteristics have been<br />
observed in the first <strong>and</strong> second generations (mother plant <strong>and</strong> sucker)<br />
suggesting that this characteristic is heritable to the subsequent generations. A<br />
sigmoid drooping leaf is a peculiar characteristic in certain Musa cultivars<br />
such as ‘FHIA-03’, which is a cooking banana (AABB) with high production<br />
<strong>and</strong> good vigor (Reyes-Borja, 1995). Un<strong>for</strong>tunately, bunch characteristics of<br />
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