Gamma Rays and CarbonIon-Beams Irradiation for Mutation ...

Gy. ‘Williams’ and ‘Orito’ could not tolerate radiation dosages more than
200 Gy. However, the height in ‘Williams’ remained similar even when
doses were increased to 50, 100 and 150 (Fig 4, B). Difference between
‘Cavendish Enano’ and ‘FHIA-01’ was significant at the 5% level (P≦
0.05), and those between ‘Williams’ and ‘Orito’ was significant at the 1%
level (P ≦ 0.01). ‘Cavendish Enano’ across dosages was significantly
difference (P≦0.05).
Fig. 4, C shows the relationship between survival rate and doses
for each cultivar. The survival ability decreased across the dosage. The
survival rate of ‘Cavendish Enano’, ‘FHIA-01’ and ‘Orito’, at 50 Gy was
significantly different from that of ‘Williams’ with higher survival rate
(Table 3). However, when dose was increased to 100 Gy, ‘Cavendish
Enano’ had significantly higher survival rate than ‘Williams’, ‘FHIA-01’
and ‘Orito’. At 150 Gy, the survival rate in ‘Williams’ remained high,
while it was still low in ‘Cavendish Enano’, ‘FHIA-01’ and ‘Orito’. Above
200 Gy, ‘FHIA-01’ showed a relatively high survival rate, over the rest of
the cultivars tested. A dosage of 500 Gy resulted in the death irrespective
of cultivars used, except in ‘FHIA-01’.
Fig. 9, A and B, (‘Cavendish Enano’ and ‘Williams’,
respectively) and Fig. 10, A and B (‘Orito’ and ‘FHIA-01’, respectively)
show the survival rate (%) for LD50 as an analysis to assess acute
sensitivity in the four cultivars. LD50 was highest in ‘Williams’ (83.94%),
and lowest in ‘Orito’ (65.0%). Plus/minus (±) 5% was aggregated to each
LD50 values of each cultivar to select the optimum irradiation doses of
Gamma rays. Subsequently, the optimum doses for ‘Cavendish Enano’
ranged from 74.0 to 81.8 Gy, ‘Williams’ from 79.7 to 88.1 Gy, ‘Orito’
from 61.8 to 68.3 Gy, and ‘FHIA-01’ from 73.2 to 80.9 Gy. Fig. 11 shows
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