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Gamma Rays and CarbonIon-Beams Irradiation for Mutation ...

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‘Williams’ (B) was obtained by exponential regressions. The LD50 <strong>for</strong><br />

‘Williams’ was at 13.5 Gy <strong>and</strong> ‘Cavendish Enano’ at 15.0 Gy, indicating<br />

the <strong>for</strong>mer would be more sensitive to the ion-beams than the latter. Figs.<br />

38 <strong>and</strong> 39 shows banana plantlets of ‘Cavendish Enano’ <strong>and</strong> ‘Williams’<br />

affected by different doses of ion beam (Gy), 19 days after irradiation.<br />

The results of the three cultivars (‘Williams’, ‘Orito’ <strong>and</strong><br />

‘FHIA-01’) used <strong>for</strong> the second irradiation is mentioned as following. The<br />

analysis of variance <strong>for</strong> survival rate shows significant differences among<br />

the ion-beam doses, but there is no difference between cultivars. Tukey<br />

analysis (P≦0.05) reported that there are two groups in which the means<br />

are not significantly different from one another. The analysis of variance<br />

<strong>for</strong> the numbers of shoot per explants reported significant differences<br />

between cultivars <strong>and</strong> among ion-beams doses, as well as Tukey analysis<br />

(P≦0.05). The analysis of variance <strong>for</strong> the height of shoot reported<br />

significant differences between cultivars but there is not any difference<br />

among ion-beams doses. In contrast, the analysis of variance <strong>for</strong> the weight<br />

of shoot reported significant differences among ion-beams doses, but there<br />

is not difference between cultivars.<br />

Fig. 40 is shown the shoot cluster’s weight (A), shoot cluster’s<br />

height (B), number of shoot cluster’s (C) <strong>and</strong> survival rate (D) of<br />

‘Williams’, ‘Orito’ <strong>and</strong> ‘FHIA-01’ exposed to different doses of carbon<br />

ion-beams. The survival rate of the explants were similar when 0-8 Gy<br />

were applied, but 16 Gy this variable was affected. The numbers of shoot<br />

per clusters were different in each cultivar. ‘Orito’ showed higher values up<br />

to 8 Gy, however ‘Williams’ showed higher values when 16 Gy were<br />

applied. Shoot cluster’s weight <strong>and</strong> shoot cluster’s height showed a similar<br />

tendency across the ion-beams doses. Regenerated explants <strong>and</strong> cluster are<br />

shown in Fig 41.<br />

84

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