an epidemiological study of listeriosis in dairy cattle

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L. denitrificans from the genus. After serological studies and other related taxonomic works L. murrayi and L. grayi were kept within the genus (Rocourt and others 1987, Farber and Peterkins 1991). At the present time, the genus Listeria has seven species which are L. monocytogenes, L. ivanovii, L. seeligeri, L. innocua, L. welshimeri, L. murrayi and L. grayi. Although Listeria monocytogenes is widely considered to be the only pathogen of people and animals, L. ivanovii has been associated with abortion in sheep and rarely in cattle (Alexander and others 1992), L. seeligeri has been isolated from cases of encephalitis in people and L. innocua has been implicated in some encephalitic cases both naturally and experimentally in ruminants (Walker and others 1994). The other species are believed to be non pathogenic (McLauchlin 1987). 7
Table 1. 2. Serovar distribution of Listeria Paterson Seeliger - Donker-Voet O antigen H antigen 1 1/2a I II (III) AB 1/2b I II (III) ABC 2 1/2c I II (III) BD 3a II (III) AB 3 3b II (III) IV (XII) (XIII) ABC 3c II (III) IV (XII) (XIII) BD 4a (III) (V) VII IX ABC 4b (III) V VI ABC 4c (III) V VII ABC 4d (III) (V) VI VIII ABC 4 4e (III) V VI (VIII) (IX) ABC 5 (III) (V) VI VIII X ABC 6a (4f) (III) V (VI) (VII) (IX) (XV) ABC 6b (4g) (III) (V) (VI) (VII) IX X XI ABC 7 (III) XII XIII ABC L. grayi (III) XII XIV E L. murrayi ( ) = not always present (III) XII (XIV) E 8
Page 1 and 2: AN EPIDEMIOLOGICAL STUDY OF LISTERI
Page 3 and 4: In the second part of the study a l
Page 5 and 6: I would like to thank to Metin Oztu
Page 7 and 8: DECLARATION I declare that apart fr
Page 9 and 10: 2. 2. Materials and Methods 47 2. 2
Page 11 and 12: 4. 4. Discussion 125 CHAPTER 5 A pi
Page 13 and 14: CHAPTER 1 LIST OF TABLES AND FIGURE
Page 15 and 16: Table 3. 11. The relationship betwe
Page 17 and 18: CHAPTER 5 Table 5. 1. Date of visit
Page 19 and 20: Figure 6. 4. The monthly faecal exc
Page 21 and 22: Listeralla hepatolytica. In the sam
Page 23 and 24: Listeriolysin O (Fernandaz-Garayzab
Page 25: L. monocytogenes was the only recog
Page 29 and 30: Serotyping is based on the identifi
Page 31 and 32: electrophoresis. Different strains
Page 33 and 34: and others (1995) came to the concl
Page 35 and 36: animals suggests that L. monocytoge
Page 37 and 38: Immunity reducing Septicaemia facto
Page 39 and 40: monocytogenes and a prerequisite to
Page 41 and 42: a variable number of cases of encep
Page 43 and 44: The relationship between age and Li
Page 45 and 46: a reservoir from which it spreads t
Page 47 and 48: 1. 8. Clinical signs and pathology
Page 49 and 50: is seldom clinical illness in the d
Page 51 and 52: Bacterial culture is widely used fo
Page 53 and 54: Differential diagnosis: Listerial m
Page 55 and 56: Since elimination of L. monocytogen
Page 57 and 58: first description, it was only 1980
Page 59 and 60: and meat products have resulted in
Page 61 and 62: infectious dose for the infection i
Page 63 and 64: a longitudinal study involving five
Page 65 and 66: in Britain. In this part of the stu
Page 67 and 68: groups were excluded from analysis.
Page 69 and 70: The proportions of farms with cases
Page 71 and 72: Table 2. 3. The proportion of anima
Page 73 and 74: percentage 50 45 40 35 30 25 20 15
Page 75 and 76: Milking cows (%) Rep. heifers (%) D
Page 77 and 78:
Abortion 274 (57.8%) 28 (21.9%) Ner
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with others (Kanuk and Berenson 197
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occurred in replacement heifers and
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CHAPTER 3 The relationship between
Page 85 and 86:
(ii) Cases in milking cows: Farms r
Page 87 and 88:
To assess the relationship between
Page 89 and 90:
) Duration of housing: If animals w
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d) Presence of moles: Farmers were
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listeriosis. Maize silage feeding a
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found between the duration of feedi
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a) Type of housing : Housing animal
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Herd sizes n Y N OR P value x Y N O
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of disease. Similarly storing feed
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a) Type of forages: Feeding maize s
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) Use of bedding: Using straw beddi
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) Source of forages: Home made gras
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1.29-18.22) whereas straw bedding i
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100 2 34 2.33 0.01 X 2 22 3.35 0.01
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a) Dung disposal: Storage of manure
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vaccination against Leptospirosis +
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suppression of the growth of Lister
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1989, Sargison 1993). It is also po
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L. monocytogenes has been isolated
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association (Kirkwood 1988, Thrusfi
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The predictor variables that met ou
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Forage feeding Maize silage Hay Str
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indoor feeding fed ad libitum on th
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cases of Listeriosis in sheep 2.9 1
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and controlling moles in fields wer
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Cases of Listeriosis in beef cattle
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This model was similar to the Model
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Table 4. 9. The multivariate relati
Page 141 and 142:
5) Nervous signs: Cases of Listerio
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feeders, maize silage feeding in ri
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A contradictory finding was made ab
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selective enrichment broth, at refr
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growth of some strains of Listeria
Page 151 and 152:
5. 2. 2. Bacteriology : a) Culture
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culturing negative LSEB (1/10w/v) s
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6) Sugar tests: Listeria are capabl
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uffered saline (PBS), pH 7.2, centr
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Investigation of non-specific bindi
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and 0.94 for L. monocytogenes (17/1
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Detection limit of the method: A si
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Relationship between housing, silag
Page 167 and 168:
5 13 3 3 1 3 1 1 1 6 14 1 4 5 3 1 0
Page 169 and 170:
Table 5. 11. The relationship betwe
Page 171 and 172:
frequency of isolation of L. monocy
Page 173 and 174:
CHAPTER 6 A study of the dynamic of
Page 175 and 176:
There were no sample size calculati
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4 of the farms had their silage ana
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a) Faecal samples: Faecal samples w
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• • • 1 5 9 6. 2. 5. Sample p
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A total of 944 isolates of L. monoc
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UK), 0.5μM DNA primer, 1 unit Supe
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entered and left the herd over the
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Table 6. 7. Monthly frequency of ex
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Table 6. 8. The relationship betwee
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(MA) mean age, (n) number, (R) rang
Page 195 and 196:
frequency of excretion (P
Page 197 and 198:
There was no evidence from this far
Page 199 and 200:
) Environmental samples: Both L. in
Page 201 and 202:
monocytogenes. By April the proport
Page 203 and 204:
A total of 211 milking cows were ex
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100 % 90 80 70 60 50 40 30 20 10 0
Page 207 and 208:
The number of new cases each month
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monocytogenes was also isolated fro
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Table 6. 18 The isolates, their ori
Page 213 and 214:
Table 6. 19. The distribution of th
Page 215 and 216:
On farm C pattern 1 was detected in
Page 217 and 218:
Figure 6. 8. The repeatability of R
Page 219 and 220:
Figure 6. 10. The distribution of s
Page 221 and 222:
Figure 6. 12a. The RAPD pattern obt
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1988, Husu 1990) and from zero to a
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experimentally been shown that ther
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periods. However, on farms B and E
Page 229 and 230:
A large number of L. monocytogenes
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Figure 6. 13. Animal-environment cy
Page 233 and 234:
Some important farming practices we
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chance finding of L. monocytogenes
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Asahi, O., Hosoda, T. and Akuyama,
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Bourry, A. and Poutrel, B. (1996) B
Page 241 and 242:
Chakraborty, T., Ebel, F., Wehland,
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delGarso, L. and Wallop, W. (1975)
Page 245 and 246:
Eveland, W.C. (1963) Demonstration
Page 247 and 248:
Fraser, J.A. and Sperber, W.H. (198
Page 249 and 250:
Golden, D.A, Beuchat, L.R. and Brac
Page 251 and 252:
humans. In Miller, A.J.,. Smith, J.
Page 253 and 254:
Jones, F.S. and Little, R.B. (1934)
Page 255 and 256:
Listeria innocua by oral inoculatio
Page 257 and 258:
Low, J.C. and Donachie, W.A. (1997)
Page 259 and 260:
McBride, M.E. and Girard, K.F. (196
Page 261 and 262:
Nieman, R.E. and Lorber, B. (1980)
Page 263 and 264:
Peters, M., Pohlenz, J., Jaton, K.,
Page 265 and 266:
Bakteriologie, Mikrobiologie und Hy
Page 267 and 268:
Seeliger, H.P.R. (1981) Apathogenic
Page 269 and 270:
Tilney, L.G. and Portnoy, D.A. (198
Page 271 and 272:
Walker, J.K., Morgan, J.H., McLauch
Page 273 and 274:
APPENDIX 3 PREPARATION OF BACTERIOL
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Cool to 50 0 C Mix Solution A with
Page 277 and 278:
Phosphate buffered saline (PBS; 10x
Page 279 and 280:
APPENDIX 2 THE OVERALL RESULTS OF U
Page 281 and 282:
Grass silage Y N OR (95% CL) p Valu
Page 283 and 284:
storage Y N OR (95% CL) p Value cla
Page 285 and 286:
source home made 38 182 0.6 (0.1-16
Page 287 and 288:
source home made 34 345 0.8 (0.3-2.
Page 289 and 290:
source barley 27 251 0.93(0.6-1.6)
Page 291 and 292:
source potatoes 2 11 1.4 (0.-7.7) 0
Page 293 and 294:
in a covered barn 73 595 0.8 (0.4-1
Page 295 and 296:
sawdust 0 16 0.0 (0.0-2.73) 0.3 str
Page 297 and 298:
APPENDIX 5 THE QUESTIONNAIRE AND TH
Page 299 and 300:
Abbreviations: 1 first blood sampli
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B 113 1 114 1 115 1 116 1 117 1 118
Page 303 and 304:
B 104 1 104 2 104 3 105 1 105 2 105
Page 305 and 306:
B 20 1 20 2 20 3 21 1 21 2 21 3 19
Page 307 and 308:
0.169 1.157 1.134 1.467 1.356 0.145
Page 309 and 310:
Plate 4 Layout and ODs B 73 1 73 2
Page 312 and 313:
UNIVERSITY OF BRISTOL LISTERIOSIS I
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PART C : HERD SIZE :--BETWEEN JULY
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(please circle one for each) 3. Wha
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etween which months were your milki
Page 320 and 321:
not stored beneath the slats compos
Page 322 and 323:
STUDY OF LISTERIOSIS IN DAIRY CATTL
Page 324 and 325:
STUDY OF LISTERIOSIS IN DAIRY CATTL
Page 326 and 327:
Now I would like to get some detail
Page 328 and 329:
Root crops : ______________________
Page 330 and 331:
In this section I will be asking qu
Page 332 and 333:
33. Which days of the week bulk tan
Page 334 and 335:
-what was used to treat? -what was
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an epidemiological study of listeriosis in dairy cattle

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