an epidemiological study of listeriosis in dairy cattle

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93 of the total number of respondents (894) reported cases of clinical Listeriosis in their dairy cattle between June 1994 and July 1995. 26 farm level predictor variables were associated with disease, 21 with an increased and 5 with a decreased risk. 2. (i) Herd size: The overall results of the univariate analysis are also presented in the appendix There was a statistically significant association between the number of milking cows in a herd and the occurrence of disease. The risk of reporting disease increased as the herd size increased (Table 3. 2.). Table 3. 2. Univariate relationship between herd sizes and clinical listeriosis Herd sizes n June 1995 July 1994 Y N OR P value X Y N OR P value X M. cows 100 31 141 3.26
listeriosis. Maize silage feeding also increased the risk of disease in dairy cattle and the Odds Ratio for this variable was 2.4 (95% CL 1.50-3.85). There was also a statistically significant association between feeding straw when animals were housed and a decreased risk of reporting disease in dairy cattle (OR 0.38, 95% CL 0.18-0.78). (Table 3. 3). When the individual types of straw (barley, wheat, wheat and barley and the combination of wheat, barley, oat and pea) or root crops (beet type, potatoes, brassica type, kale and others) were taken into consideration there was no association between these and the occurrence of listeriosis in dairy cattle. b) Sources of forage: Farmers were given three alternative sources of forage; purchased, home made and any other sources. There was a statistically significant association between purchased grass silage (OR 2.91, CL 1.22-6.8) and increased risk of reporting Listeriosis (Table 3. 3). c) Methods of feeding forages: 1) outdoor feeding: Feeding maize silage (OR 1.35, CL 1.59-13.04), hay (OR 2.98, CL 1.12-8.23) and straw (OR 3.72, CL 1.25-11.88) in ring feeders when animals were out were associated with an increased risk of disease (Table 3. 3). 2) indoor feeding: Two methods (feeding forages in ring feeders and on the floor) were associated with disease. Feeding grass silage (OR 1.93, CL 1.21-3.07), maize silage (OR 4.96, CL 2.17-11.46) and hay (OR 4.17, CL 1.83-9.61) in ring feeders was positively associated with disease whereas feeding grass silage (OR 0.23, CL 0.04-0.97) and hay (OR 0.0, CL 0.0-0.85) on the floor was negatively associated with disease (Table 3. 3). 75
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AN EPIDEMIOLOGICAL STUDY OF LISTERI
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In the second part of the study a l
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I would like to thank to Metin Oztu
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DECLARATION I declare that apart fr
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2. 2. Materials and Methods 47 2. 2
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4. 4. Discussion 125 CHAPTER 5 A pi
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CHAPTER 1 LIST OF TABLES AND FIGURE
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Table 3. 11. The relationship betwe
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CHAPTER 5 Table 5. 1. Date of visit
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Figure 6. 4. The monthly faecal exc
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Listeralla hepatolytica. In the sam
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Listeriolysin O (Fernandaz-Garayzab
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L. monocytogenes was the only recog
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Table 1. 2. Serovar distribution of
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Serotyping is based on the identifi
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electrophoresis. Different strains
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and others (1995) came to the concl
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animals suggests that L. monocytoge
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Immunity reducing Septicaemia facto
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monocytogenes and a prerequisite to
Page 41 and 42: a variable number of cases of encep
Page 43 and 44: The relationship between age and Li
Page 45 and 46: a reservoir from which it spreads t
Page 47 and 48: 1. 8. Clinical signs and pathology
Page 49 and 50: is seldom clinical illness in the d
Page 51 and 52: Bacterial culture is widely used fo
Page 53 and 54: Differential diagnosis: Listerial m
Page 55 and 56: Since elimination of L. monocytogen
Page 57 and 58: first description, it was only 1980
Page 59 and 60: and meat products have resulted in
Page 61 and 62: infectious dose for the infection i
Page 63 and 64: a longitudinal study involving five
Page 65 and 66: in Britain. In this part of the stu
Page 67 and 68: groups were excluded from analysis.
Page 69 and 70: The proportions of farms with cases
Page 71 and 72: Table 2. 3. The proportion of anima
Page 73 and 74: percentage 50 45 40 35 30 25 20 15
Page 75 and 76: Milking cows (%) Rep. heifers (%) D
Page 77 and 78: Abortion 274 (57.8%) 28 (21.9%) Ner
Page 79 and 80: with others (Kanuk and Berenson 197
Page 81 and 82: occurred in replacement heifers and
Page 83 and 84: CHAPTER 3 The relationship between
Page 85 and 86: (ii) Cases in milking cows: Farms r
Page 87 and 88: To assess the relationship between
Page 89 and 90: ) Duration of housing: If animals w
Page 91: d) Presence of moles: Farmers were
Page 95 and 96: found between the duration of feedi
Page 97 and 98: a) Type of housing : Housing animal
Page 99 and 100: Herd sizes n Y N OR P value x Y N O
Page 101 and 102: of disease. Similarly storing feed
Page 103 and 104: a) Type of forages: Feeding maize s
Page 105 and 106: ) Use of bedding: Using straw beddi
Page 107 and 108: ) Source of forages: Home made gras
Page 109 and 110: 1.29-18.22) whereas straw bedding i
Page 111 and 112: 100 2 34 2.33 0.01 X 2 22 3.35 0.01
Page 113 and 114: a) Dung disposal: Storage of manure
Page 115 and 116: vaccination against Leptospirosis +
Page 117 and 118: suppression of the growth of Lister
Page 119 and 120: 1989, Sargison 1993). It is also po
Page 121 and 122: L. monocytogenes has been isolated
Page 123 and 124: association (Kirkwood 1988, Thrusfi
Page 125 and 126: The predictor variables that met ou
Page 127 and 128: Forage feeding Maize silage Hay Str
Page 129 and 130: indoor feeding fed ad libitum on th
Page 131 and 132: cases of Listeriosis in sheep 2.9 1
Page 133 and 134: and controlling moles in fields wer
Page 135 and 136: Cases of Listeriosis in beef cattle
Page 137 and 138: This model was similar to the Model
Page 139 and 140: Table 4. 9. The multivariate relati
Page 141 and 142: 5) Nervous signs: Cases of Listerio
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feeders, maize silage feeding in ri
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A contradictory finding was made ab
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selective enrichment broth, at refr
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growth of some strains of Listeria
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5. 2. 2. Bacteriology : a) Culture
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culturing negative LSEB (1/10w/v) s
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6) Sugar tests: Listeria are capabl
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uffered saline (PBS), pH 7.2, centr
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Investigation of non-specific bindi
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and 0.94 for L. monocytogenes (17/1
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Detection limit of the method: A si
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Relationship between housing, silag
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5 13 3 3 1 3 1 1 1 6 14 1 4 5 3 1 0
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Table 5. 11. The relationship betwe
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frequency of isolation of L. monocy
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CHAPTER 6 A study of the dynamic of
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There were no sample size calculati
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4 of the farms had their silage ana
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a) Faecal samples: Faecal samples w
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• • • 1 5 9 6. 2. 5. Sample p
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A total of 944 isolates of L. monoc
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UK), 0.5μM DNA primer, 1 unit Supe
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entered and left the herd over the
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Table 6. 7. Monthly frequency of ex
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Table 6. 8. The relationship betwee
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(MA) mean age, (n) number, (R) rang
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frequency of excretion (P
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There was no evidence from this far
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) Environmental samples: Both L. in
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monocytogenes. By April the proport
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A total of 211 milking cows were ex
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100 % 90 80 70 60 50 40 30 20 10 0
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The number of new cases each month
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monocytogenes was also isolated fro
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Table 6. 18 The isolates, their ori
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Table 6. 19. The distribution of th
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On farm C pattern 1 was detected in
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Figure 6. 8. The repeatability of R
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Figure 6. 10. The distribution of s
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Figure 6. 12a. The RAPD pattern obt
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1988, Husu 1990) and from zero to a
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experimentally been shown that ther
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periods. However, on farms B and E
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A large number of L. monocytogenes
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Figure 6. 13. Animal-environment cy
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Some important farming practices we
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chance finding of L. monocytogenes
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Asahi, O., Hosoda, T. and Akuyama,
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Bourry, A. and Poutrel, B. (1996) B
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Chakraborty, T., Ebel, F., Wehland,
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delGarso, L. and Wallop, W. (1975)
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Eveland, W.C. (1963) Demonstration
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Fraser, J.A. and Sperber, W.H. (198
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Golden, D.A, Beuchat, L.R. and Brac
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humans. In Miller, A.J.,. Smith, J.
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Jones, F.S. and Little, R.B. (1934)
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Listeria innocua by oral inoculatio
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Low, J.C. and Donachie, W.A. (1997)
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McBride, M.E. and Girard, K.F. (196
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Nieman, R.E. and Lorber, B. (1980)
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Peters, M., Pohlenz, J., Jaton, K.,
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Bakteriologie, Mikrobiologie und Hy
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Seeliger, H.P.R. (1981) Apathogenic
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Tilney, L.G. and Portnoy, D.A. (198
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Walker, J.K., Morgan, J.H., McLauch
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APPENDIX 3 PREPARATION OF BACTERIOL
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Cool to 50 0 C Mix Solution A with
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Phosphate buffered saline (PBS; 10x
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APPENDIX 2 THE OVERALL RESULTS OF U
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Grass silage Y N OR (95% CL) p Valu
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storage Y N OR (95% CL) p Value cla
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source home made 38 182 0.6 (0.1-16
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source home made 34 345 0.8 (0.3-2.
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source barley 27 251 0.93(0.6-1.6)
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source potatoes 2 11 1.4 (0.-7.7) 0
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in a covered barn 73 595 0.8 (0.4-1
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sawdust 0 16 0.0 (0.0-2.73) 0.3 str
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APPENDIX 5 THE QUESTIONNAIRE AND TH
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Abbreviations: 1 first blood sampli
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B 113 1 114 1 115 1 116 1 117 1 118
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B 104 1 104 2 104 3 105 1 105 2 105
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B 20 1 20 2 20 3 21 1 21 2 21 3 19
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0.169 1.157 1.134 1.467 1.356 0.145
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Plate 4 Layout and ODs B 73 1 73 2
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UNIVERSITY OF BRISTOL LISTERIOSIS I
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PART C : HERD SIZE :--BETWEEN JULY
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(please circle one for each) 3. Wha
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etween which months were your milki
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not stored beneath the slats compos
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STUDY OF LISTERIOSIS IN DAIRY CATTL
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STUDY OF LISTERIOSIS IN DAIRY CATTL
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Now I would like to get some detail
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Root crops : ______________________
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In this section I will be asking qu
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33. Which days of the week bulk tan
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-what was used to treat? -what was
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an epidemiological study of listeriosis in dairy cattle

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