of the Max - MDC

Structure of the Group
Group Leader
Prof. Dr. Wolfgang Uckert
Scientists
Dr. Boris Engels*
Dr. Haike Gelfort
Dr. Lilian Stärk*
Graduate and
Undergraduate Students
Petra Biese*
Florian Helm*
Elisa Kieback
Matthias Leisegang
Kristina Mahnken*
Simone Reuß
Nicole Scheumann*
Daniel Sommermeyer
Technical Assistants
Uta Fischer
Martina Grabbert
Kordelia Hummel
Janina Kunze*
Irmgard Küttner
Secretariat
Romana Worm
* part of the period reported
A
B
Figure 2. Prevention of autoimmune diabetic
disease mediated by myc-antibody depletion. (A)
B6 splenocytes were transduced with either OT-
I/TCRwt (n=5) or OT-I/TCRmyc (n=10) and 2x10 7
TCR-positive cells were injected i.v. into sublethally
irradiated RIP-mOVA mice. Mice which were irradiated
but received no cells served as a negative control
(n=3). Two days after adoptive transfer 500 µg of
an anti-myc antibody was administered i.p. into all
mice which had received T cells harboring the TCRwt
and half of the mice (n=5) which had received T
cells carrying the TCRmyc (arrow). The other half
(n=5) which had received OT-I/TCRmyc-transduced
cells was not treated with antibody. Mice with blood
glucose levels higher than 14 mM are considered
diabetic. Depicted are mean values of all animals in
one group, error bars show the standard deviation.
If measurement exceeded the upper detection limit
of 33.3 mM, values were set as 35 mM to allow the
calculation of mean blood glucose levels. (B) Ten
days after adoptive transfer pancreases of mice from
each group were analyzed by immunohistochemistry
with a CD8-specific antibody. In antibody-treated
mice no remaining CD8-positive T cells were found in
β-islets.
Selected Publications
Engels, B, Nößner, E, Frankenberger, B, Blankenstein, T,
Schendel, D, Uckert, W. (2005). Redirecting human T lymphocytes
towards renal cell carcinoma-specificity by retroviral transfer
of T cell receptor genes. Hum. Gene Ther. 16, 799-810.
Xue, S-A. Gao, L, Hart, D, Gillmore, R, Qasim, W, Thrasher, A,
Apperley, J, Engels, B, Uckert, W, Morris, E, Stauss, H. (2005).
Elimination of human leukemia cells in NOD/SCID mice by
WT1-TCR gene-transduced human T cells. Blood 106, 3062-3067.
Sommermeyer, D, Neudorfer, J, Weinhold, M, Leisegang, M,
Charo, J, Engels, B, Nößner, E. Heemskerk, M, Schendel, DJ,
Blankenstein, T, Bernhard, H, Uckert, W. (2006). Designer T
cells by T cell receptor replacement. Eur. J. Immunol. 36,
3052-3059.
Reuss, S, Biese, P, Cosset, FL, Takeuchi, Y, Uckert, W. (2007).
Suspension packaging cell lines for the simplified generation of
T cell receptor encoding retrovirus vector particles. Gene Therapy
14, 595-603.
Engels, B, Uckert, W. (2007). Redirecting T lymphocyte specificity
by T cell receptor gene transfer – A new era for immunotherapy.
Mol. Aspects Med. 28, 115-142.
Cancer Research 139