Thesis final - after defense-7 - Jacobs University

Chapter 3
among the adsorbents were higher at low salt concentrations than at the high salt
concentrations (121). The reason for this could be that at the extreme hydrophobic conditions,
the hydrophobic potential of the mobile phase has covered the potential of the base supports
to demonstrate according to their hydrophobicities. At low salt concentrations, the mobile
phase has low hydrophobicity and the adsorption was more dependent on the hydrophobicity
of the base supports.
3.2.3.5. Application note
This work can help those interested in the purification of proteins listed in Tables 12-14. It
will simplify the work of researchers and they will not have to scout for chromatographic
experiments to figure out the elution position of any tabulated protein. The protein can be
purified by isocratic chromatography, without going through trial and error chromatographies.
Some of the important enzymes are Pyruvate kinase (eluted at 0.6 M on Sepharose-Phenyl),
Phosphoglycerate mutase 2 (eluted at 0.8 M on Toyopearl-Phenyl), Polymerase A (0 M on
Source-Pheny), Methyl transferase (0.2 M on Sepharose-Phenyl), Thiosulfate sulphur
transferase (0.2 M on Source-Phenyl), Enolase 2 (1.6 M on Toyopearl-Phenyl), D-serine
dehydratase (0.6 M on Toyopearl-Phenyl) and Alcohol dehydrogenase (0.2 M on Sepharose-
Phenyl).
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