Thesis final - after defense-7 - Jacobs University

Chapter 3
Table 6. List of the identified proteins fractionated by Toyopearl-Butyl and their characterization with different properties
Prot A Salt B Prot ≠ C Prot. I.D D Mr E pI F PDB G ASH H AH I AH J AH K AP L AP M AF N AB O
Low 1.6 A1-TB DBF20_YEAST 65 8.68 2LAH 0.3864 0.399 0.501 0.584 0.471 0.320 0.605 0.647
A2-TB YP191_YEAST 41 5.55 3C9P 0.4087 0.415 0.440 0.603 0.475 0.301 0.596 0.649
A3-TB SMA1_YEAST 28 9.85 3RDJ 0.4189 0.382 0.448 0.565 0.465 0.319 0.606 0.653
A4-TB YSY6_YEAST 7 10.43 3NYB 0.4192 0.388 0.450 0.588 0.560 0.279 0.583 0.655
1.4 B1-TB RDS2_YEAST 50 7.87 2KOA 0.4234 0.384 0.499 0.581 0.475 0.281 0.588 0.636
B2-TB PABP_YEAST 64 5.71 2X4T 0.4244 0.371 0.499 0.590 0.489 0.277 0.592 0.613
B3-TB FMP46_YEAST 15 9.38 2QL8 0.4442 0.404 0.498 0.593 0.485 0.278 0.594 0.671
Medium
B4-TB IDHH_YEAST 47 9.24 3BLX 0.4339 0.414 0.500 0.582 0.483 0.306 0.601 0.650
1.0 C1-TB TAF5_YEAST 88 7.01 3HMH 0.4447 0.374 0.502 0.595 0.483 0.306 0.590 0.620
C2-TB GFD1_YEAST 21 9.76 3LCN 0.4434 0.382 0.505 0.583 0.485 0.373 0.596 0.626
C3-TB YDL052C 33 9.67 3R0S 0.4412 0.384 0.499 0.588 0.475 0.312 0.575 0.668
C4-TB UBP4_YEAST 105 8.50 3IRT 0.4471 0.392 0.512 0.604 0.469 0.299 0.603 0.650
0.8 D1-TB Vtil1p 10 4.82 3ONJ 0.4447 0.349 0.501 0.589 0.476 0.323 0.596 0.642
D2-TB LDB7_YEAST 19 9.08 2R10 0.4457 0.388 0.507 0.594 0.466 0.291 0.598 0.605
D3-TB TFC4p 120 5.14 3MA5 0.4573 0.408 0.495 0.592 0.478 0.288 0.595 0.678
D4-TB DRS1_YEAST 85 5.42 3RC3 0.4593 0.405 0.512 0.602 0.465 0.285 0.602 0.626
D5-TB TFC1_YEAST 73 5.23 2JO4 0.4619 0.408 0.506 0.605 0.481 0.283 0.603 0.651
0.6 E1-TB HIM1_YEAST 47 9.15 3P82 0.4629 0.410 0.524 0.608 0.453 0.259 0.609 0.658
E2-TB TEX1_YEAST 47 8.88 3MXJ 0.4630 0.414 0.528 0.615 0.450 0.256 0.607 0.635
E3-TB FAF1_YEAST 38 9.33 3R3M 0.4620 0.405 0.526 0.609 0.456 0.275 0.613 0.585
E4-TB TDH3p 35 6.46 1Q32 0.4647 0.405 0.534 0.607 0.450 0.255 0.608 0.627
E5-TB RPN2_YEAST 104 5.85 2X5N 0.4712 0.417 0.534 0.637 0.449 0.258 0.610 0.639
High 0.2 F1-TB ICP55_YEAST 57 6.11 1VZ2 0.4644 0.414 0.527 0.607 0.456 0.273 0.615 0.641
F2-TB DSD1_YEAST 47 7.53 3ANU 0.4751 0.426 0.534 0.625 0.432 0.242 0.613 0.651
F3-TB PLSC_YEAST 33 9.63 1IUQ 0.4770 0.446 0.535 0.635 0.431 0.235 0.615 0.670
F4-TB YMX6_YEAST 105 9.72 2JXN 0.4826 0.429 0.542 0.622 0.422 0.256 0.680 0.585
F5-TB MSA2_YEAST 39 10.08 3POJ 0.4731 0.412 0.526 0.615 0.468 0.268 0.634 0.629
0 G1-TB RS7A_YEAST 21 9.83 3O2Z 0.4842 0.435 0.530 0.600 0.419 0.255 0.643 0.683
G2-TB YSW1_YEAST 70 6.69 3OQC 0.5018 0.437 0.547 0.625 0.417 0.255 0.629 0.648
G3-TB IF4A_YEAST 44 5.02 2G9N 0.5082 0.433 0.532 0.618 0.447 0.228 0.680 0.659
G4-TB SGM1_YEAST 81 4.81 2YB7 0.5210 0.448 0.572 0.633 0.412 0.248 0.639 0.638
G5-TB IMP2_YEAST 19 10.01 1L0N 0.5233 0.429 0.576 0.628 0.416 0.220 0.669 0.676
A ; Protein hydrophobicity, B ; Salt concentration, C ; Identity number, D ; I.D. in SwissProt database, E ; Molecular weight, F ; Isoelectric point, G ; I.D in
PDB databank, H ; Average surface hydrophobicity calculated by Cowan-Whittaker’s scale, I, J and K ; Average hydrophobicity calculated by the scales
of Miyazawa-Jernigan, Cowan-Whittaker and Tanford, respectively. L and M ; Average polarity calculated by the scales of Grantham and Zimmerman,
respectively. N ; Average flexibility calculated by Bhaskaran and Ponnuswamy’s scale, O ; Average bulkiness calculated by Zimmerman’s scale.
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