Thesis final - after defense-7 - Jacobs University

Chapter 3
Table 7. List of the identified proteins fractionated by Toyopearl-Hexyl and their characterization with different properties
Prot A Salt B Prot ≠ C Prot. I.D D Mr E pI F PDB G ASH H AH I AH J AH K AP L AP M AF N AB O
Low 1.6 A1-TH POP1_YEAST 100 9.83 1R5D 0.3694 0.400 0.515 0.580 0.461 0.291 0.606 0.644
A2-TH MSS51_YEAST 50 8.84 315X 0.4013 0.327 0.465 0.604 0.551 0.280 0.573 0.662
A3-TH Eno1_YEAST 46 6.16 3OTR 0.4095 0.375 0.472 0.555 0.547 0.387 0.588 0.614
A4-TH Eno2_YEAST 46 5.67 2XSX 0.4130 0.376 0.476 0.558 0.542 0.372 0.579 0.613
1.4 B1-TH RAD16_YEAST 91 8.54 3AUX 0.4225 0.403 0.478 0.587 0.475 0.315 0.586 0.656
B2-TH YAE1_YEAST 16 5.22 2XE7 0.4268 0.377 0.493 0.600 0.488 0.338 0.604 0.639
B3-TH DBf2_YEAST 66 8.86 3OQ0 0.4319 0.378 0.498 0.589 0.485 0.299 0.603 0.642
Medium
B4-TH Pho85 cyclin 34 9.65 2PK9 0.4274 0.380 0.500 0.603 0.484 0.309 0.602 0.670
1.0 C1-TH AIM37_YEAST 26 9.39 21B7 0.4444 0.395 0.507 0.603 0.475 0.305 0.592 0.676
C2-TH XRN1_YEAST 175 7.04 3PIE 0.4463 0.397 0.510 0.598 0.470 0.300 0.608 0.652
C3-TH MCX1_YEAST 57 7.59 3P9D 0.4471 0.403 0.516 0.608 0.470 0.308 0.619 0.675
C4-TH DEBYG2_YEAST 35 6.46 3STH 0.4345 0.405 0.527 0.612 0.462 0.287 0.595 0.627
0.8 D1-TH TAF5_YEAST 88 7.01 3HMH 0.4447 0.406 0.502 0.595 0.483 0.273 0.597 0.620
D2-TH YO316_YEAST 07 11.66 3PLU 0.4534 0.409 0.515 0.604 0.468 0.296 0.604 0.627
D3-TH UB4_YEAST 105 8.50 3IRT 0.4471 0.410 0.509 0.593 0.479 0.290 0.603 0.650
D4-TH YL345_YEAST 58 6.01 2DWO 0.4536 0.414 0.520 0.611 0.464 0.295 0.594 0.659
D5-TH AI2M_YEAST 97 9.67 3IFJ 0.4567 0.418 0.526 0.612 0.463 0.289 0.594 0.657
0.6 E1-TH R1R1_YEAST 99 5.83 2CVY 0.4581 0.420 0.520 0.613 0.460 0.287 0.592 0.639
E2-TH YE119_YEAST 14 8.49 1M94 0.4594 0.426 0.524 0.613 0.458 0.271 0.629 0.652
E3-TH YJHO_YEAST 104 5.29 3SDJ 0.4612 0.427 0.526 0.609 0.450 0.277 0.632 0.667
E4-TH SNZ2_YEAST 32 5.40 1DTY 0.4631 0.429 0.527 0.605 0.445 0.270 0.645 0.648
E5-TH METE_YEAST 85 6.07 3L7R 0.4660 0.430 0.534 0.621 0.452 0.276 0.644 0.655
High 0.2 F1-TH NIC96_YEAST 96 6.37 2RFO 0.4672 0.431 0.526 0.608 0.456 0.276 0.645 0.662
F2-TH YPD1_YEAST 19 4.58 1QSP 0.4697 0.432 0.530 0.612 0.450 0.270 0.618 0.656
F3-TH BOP3_YEAST 43 9.72 3PLT 0.4791 0.425 0.539 0.618 0.425 0.259 0.651 0.696
F4-TH SNF12_YEAST 63 5.47 2QLV 0.4711 0.434 0.542 0.618 0.447 0.284 0.646 0.644
F5-TH DYR_YEAST 24 7.67 3Q1H 0.4764 0.438 0.536 0.621 0.440 0.289 0.647 0.662
0 G1-TH SPS1_YEAST 55 7.55 3CZ8 0.4987 0.442 0.527 0.626 0.415 0.252 0.630 0.649
G2-TH PNG1_YEAST 42 6.22 1PNG 0.4784 0.432 0.549 0.625 0.433 0.255 0.648 0.644
G3-TH PMG1_YEAST 27 8.81 4PGM 0.4843 0.435 0.552 0.627 0.427 0.248 0.649 0.657
G4-TH RL20A_YEAST 20 10.30 3O58 0.4896 0.437 0.554 0.630 0.419 0.243 0.649 0.676
G5-TH MLP1_YEAST 21 5.14 2XMF 0.5159 0.442 0.553 0.634 0.418 0.237 0.650 0.647
A ; Protein hydrophobicity, B ; Salt concentration, C ; Identity number, D ; I.D. in SwissProt database, E ; Molecular weight, F ; Isoelectric point, G ; I.D in
PDB databank, H ; Average surface hydrophobicity calculated by Cowan-Whittaker’s scale, I, J and K ; Average hydrophobicity calculated by the scales
of Miyazawa-Jernigan, Cowan-Whittaker and Tanford, respectively. L and M ; Average polarity calculated by the scales of Grantham and Zimmerman,
respectively. N ; Average flexibility calculated by Bhaskaran and Ponnuswamy’s scale, O ; Average bulkiness calculated by Zimmerman’s scale.
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