Thesis final - after defense-7 - Jacobs University

Chapter 1
Figure 5: Flow chart describing the fractionation and characterization procedure of the crude
extract for obtaining the data about bioprocess parameters. The data produced can be stored
in databases to design the purification models (Adapted from Ref. 86).
Several researchers have reported different methodologies to predict about the retention time
of model proteins (15, 87). However, the prediction models were based on the data collected
from very few experiments and due to this reason there is still no universal scheme which can
be considered as the best one for the downstream processing. These models have several
drawbacks which can raise questions on their applicability. A major drawback was that the
experimental data for these models has been derived from the model proteins instead of a host
cell proteome. Another major drawback was that the experimental data was based on the
retention time and / or volume of the model proteins. This is not applicable to a cell proteome
because at a certain time during chromatography, several proteins are going to be eluted
instead of a single protein. The shortcomings in the previous in silico approaches have
motivated our group to report a paradigm shift from focussing on the purification of a target
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