Thesis final - after defense-7 - Jacobs University

Chapter 3
3.3.3. Partial conclusions
• A high amount and a higher number of protein spots were reported at the beginning of
the chromatography than at the end. This confirmed the less hydrophobic nature of the
S. cerevisiae cell proteome.
• The denaturation of proteins was confirmed in HIC with the proteome wide approach.
• Aromatic interactions were confirmed to have no effect on the retention behavior of
proteins during HIC.
• The effect of molecular weight of the proteins was studied in relation to the adsorbent
type. The pore size and hydrophobicity of the adsorbents were reported as the main
parameters responsible for the retention of the larger proteins.
• The effect of the isoelectric point of proteins was studied in relation to adsorbent type.
The neutral and acidic proteins have given equal distributions over 2-D gels. The
partial negative charges were confirmed on the hydrophobic adsorbents which has
given rise to ionic interactions in addition to hydrophobic interactions and resulted in
longer retention of basic proteins during chromatography.
• A higher number of acidic and smaller proteins were reported in the cell proteome in
comparison to basic and larger proteins, which can further reflect the nature of S.
cerevisiae cell proteome.
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