Thesis final - after defense-7 - Jacobs University
Thesis final - after defense-7 - Jacobs University
Thesis final - after defense-7 - Jacobs University
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Chapter 3<br />
3.1.4. Partial conclusions<br />
• The hydrophobicity of the three ligands has been investigated for the first time with<br />
the proteome wide approach utilizing different protein properties.<br />
• The ligands were not fully differentiated with the proteome wide approach, but a trend<br />
was observed.<br />
• Several protein properties such as average hydrophobicity, average flexibility, average<br />
polarity, average bulkiness and average surface hydrophobicity were investigated for<br />
their correlation with the protein retention during chromatography. Average polarity<br />
has shown an inverse relationship to the retention behavior of the proteins during HIC.<br />
Average surface hydrophobicity, average hydrophobicity and average flexibility have<br />
revealed direct relationship with the protein retention during chromatography.<br />
• Among all the properties, ASH was the only property which has a decisive role in HIC.<br />
• The large scale identification and characterization of the proteins has explored the<br />
contaminant profile of the yeast cell proteome and is an addition to the proteomics<br />
technology.<br />
• The data obtained by exploring the chromatographic behavior of the yeast cell<br />
proteome on the three different ligands can be used to design the purification models<br />
of recombinant proteins utilizing the in silico approach.<br />
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