Thesis final - after defense-7 - Jacobs University
Thesis final - after defense-7 - Jacobs University
Thesis final - after defense-7 - Jacobs University
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Chapter 3<br />
during chromatography; however it has no ability to differentiate among the ligands for their<br />
hydrophobic characters.<br />
3.1.3.3.4. Average Bulkiness (AB)<br />
The average bulkiness was calculated for the tabulated proteins employing the scale of<br />
Zimmerman (Tables 5-7). The average bulkiness has revealed no relationship (r 2 = 0.00; p =<br />
0.86) with the retention behavior of the proteins during chromatography (Figure 18). The<br />
average bulkiness values for the tabulated proteins were in a narrow range and the reason for<br />
this could be the size exclusion effect. Although bulkiness was expected to have an effect on<br />
chromatographic behavior due to its close relationship with the size of proteins, no effect was<br />
observed in the retention behavior. In the same way, the molecular weight values of the<br />
tabulated proteins have not revealed any correlation as they were representing the small<br />
fraction of the total proteins present on each 2-D gel. It was also researched by Zimmerman<br />
who proposed the scales for bulkiness and polarity, that there was no relationship between<br />
bulkiness and polarity values of the proteins according to his scales (96). In other words, if<br />
bulkiness has no correlation with polarity then it is supposed to have no relation with protein<br />
hydrophobicity. Our results confirmed that bulkiness has no role in the chromatographic<br />
separations during HIC.<br />
65
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