Thesis final - after defense-7 - Jacobs University

Chapter 3
3.2.3. Partial conclusions
• The influence of the base supports has been investigated for the first time with the
proteome wide approach utilizing different protein properties. The results revealed
that protein properties have limited ability to differentiate among the base supports.
• The base supports revealed less difference at high salt concentrations than at low salt
concentrations. In other words, the differences among the base supports were less at
high hydrophobic conditions than at low hydrophobic conditions. The reason could be
the effects of mobile phase hydrophobicity.
• Several protein properties such as average hydrophobicity, average flexibility and
average surface hydrophobicity have revealed direct relationships with the retention of
proteins. Average polarity has given an inverse relationship to the retention of proteins
during HIC.
• Among all the properties, ASH was the only property which has a decisive role in the
retention behavior of proteins during chromatography.
• The data collected from the three base supports in this process proteomics approach
can be used to design the purification models by the in silico approach.
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