Thesis final - after defense-7 - Jacobs University

Chapter 3
Ligand
Base support
(Toyopearl®)
Figure 7: The chemistry and comparison of different ligands for their hydrophobicity based on
model protein retention data (Adapted from Ref. 99).
The ligands are usually attached to toyopearl ®
base support by glycidyl ether coupling
procedures. This procedure introduces a short spacer arm to minimize steric hindrance. At the
start, HIC was a slow technique; rapid separation has been made possible by the introduction
of micro particulate semi rigid supports (Toyopearl®) (101). This revealed the importance of
toyopearl adsorbents at the industrial level. The physicochemical properties of the adsorbents
are given in Table 3 (99). The three hydrophobic adsorbents of 65-100 µm bead sizes were
selected for this study, which is the most suitable range for industrial scale purification. The
hydrophobic charm of the ligands was narrated before with toyopearl as a common base
support. However, the previous reports were based on the retention time of few model
proteins and have been never studied with the biological mixtures. Hence, it was planned to
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