Thesis final - after defense-7 - Jacobs University

Chapter 3
Table 5. List of the identified proteins fractionated by Toyopearl-Ether and their characterization with different properties
Prot A Salt B Prot ≠ C Prot. I.D D Mr E pI F PDB G ASH H AH I AH J AH K AP L AP M AF N AB O
Low 1.6 A1-TE RAD16_YEAST 91 8.54 3AUX 0.4225 0.403 0.503 0.587 0.475 0.315 0.603 0.656
A2-TE OYE2_YEAST 44 6.13 3RND 0.4234 0.390 0.524 0.606 0.465 0.274 0.612 0.638
A3-TE Fox2p 96 8.96 3QRF 0.4289 0.363 0.484 0.582 0.507 0.318 0.588 0.637
A4-TE YN92_YEAST 69 6.15 3OQ0 0.4319 0.365 0.491 0.585 0.503 0.310 0.591 0.671
1.4 B1-TE PRX1_YEAST 29 8.97 2PWJ 0.4370 0.402 0.492 0.587 0.501 0.271 0.598 0.675
B2-TE SYF1_YEAST 100 5.17 3GIX 0.4396 0.372 0.528 0.608 0.500 0.290 0.593 0.667
B3-TE TPIS_YEAST 26 5.74 3TH6 0.4411 0.408 0.503 0.597 0.454 0.302 0.599 0.688
B4-TE APM2_YEAST 69 6.41 1HES 0.4436 0.402 0.507 0.596 0.476 0.316 0.603 0.667
1.0 C1-TE UPP1 24 5.58 2EHJ 0.4451 0.382 0.511 0.605 0.478 0.279 0.597 0.662
C2-TE Fur1p 23 5.84 2JBH 0.4458 0.385 0.512 0.620 0.476 0.280 0.598 0.667
C3-TE CAT8_YEAST 160 9.13 3SNP 0.4505 0.389 0.514 0.612 0.472 0.289 0.608 0.636
C4-TE BUR1_YEAST 74 9.55 3R22 0.4495 0.390 0.511 0.614 0.478 0.284 0.624 0.649
Medium 0.8 D1-TE TRM11_YEAST 50 7.64 1ZJR 0.4516 0.411 0.517 0.616 0.462 0.288 0.612 0.639
D2-TE FSF1_YEAST 35 9.84 3OJ2 0.4584 0.412 0.518 0.617 0.461 0.279 0.614 0.648
D3-TE YO316_YEAST 7 11.66 2Y6V 0.4612 0.414 0.520 0.619 0.443 0.277 0.643 0.627
D4-TE IMH1_YEAST 105 5.52 3TCX 0.4618 0.415 0.526 0.620 0.457 0.275 0.645 0.634
D5-TE FAF1_YEAST 38 9.33 3R3M 0.4620 0.406 0.528 0.621 0.455 0.274 0.614 0.698
0.6 E1-TE ICP55_YEAST 57 6.11 1VZ2 0.4644 0.414 0.527 0.607 0.456 0.273 0.615 0.641
E2-TE CORO_YEAST 72 5.64 2AQ5 0.4660 0.417 0.531 0.622 0.448 0.264 0.636 0.629
E3-TE SPC2_YEAST 20 9.22 3SBT 0.4684 0.426 0.539 0.619 0.435 0.285 0.628 0.684
E4-TE MSA2_YEAST 39 10.08 3POJ 0.4731 0.424 0.526 0.629 0.468 0.260 0.634 0.629
E5-TE BOP3_YEAST 43 9.72 3PLT 0.4791 0.426 0.539 0.632 0.439 0.259 0.651 0.615
High 0.2 F1-TE ATG23_YEAST 51 5.77 2ZPN 0.4705 0.422 0.534 0.628 0.443 0.259 0.631 0.676
F2-TE ATG2_YEAST 178 5.62 2DYT 0.4771 0.427 0.531 0.622 0.454 0.261 0.637 0.652
F3-TE RRS1_YEAST 22 9.46 2 HBL 0.4884 0.432 0.543 0.635 0.436 0.257 0.634 0.646
F4-TE Fsf1p 31 9.84 3L06 0.4936 0.438 0.579 0.643 0.414 0.255 0.635 0.649
F5-TE SPS1_YEAST 55 7.55 3CZ8 0.4987 0.436 0.547 0.638 0.454 0.252 0.635 0.660
0 G1-TE SHU1_YEAST 171 7.88 3R7W 0.5065 0.438 0.570 0.637 0.405 0.213 0.636 0.616
G2-TE SC61G_YEAST 8 9.48 2WW9 0.5119 0.445 0.543 0.620 0.445 0.248 0.642 0.670
G3-TE PDE2_YEAST 60 6.14 1MCO 0.5187 0.449 0.547 0.622 0.421 0.242 0.643 0.675
G4-TE NUP53_YEAST 52 9.53 3P3D 0.6023 0.448 0.572 0.645 0.415 0.262 0.644 0.613
G5-TE COX1 Protein 90 9.67 3OMA 0.6336 0.439 0.543 0.604 0.435 0.270 0.647 0.655
A ; Protein hydrophobicity, B ; Salt concentration, C ; Identity number, D ; I.D. in SwissProt database, E ; Molecular weight, F ; Isoelectric point, G ; I.D in
PDB databank, H ; Average surface hydrophobicity calculated by Cowan-Whittaker’s scale, I, J and K ; Average hydrophobicity calculated by the scales
of Miyazawa-Jernigan, Cowan-Whittaker and Tanford, respectively. L and M ; Average polarity calculated by the scales of Grantham and Zimmerman,
respectively. N ; Average flexibility calculated by Bhaskaran and Ponnuswamy’s scale, O ; Average bulkiness calculated by Zimmerman’s scale.
52