Thesis final - after defense-7 - Jacobs University
Thesis final - after defense-7 - Jacobs University
Thesis final - after defense-7 - Jacobs University
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Chapter 3<br />
where approximately 50% of proteins were washed out during flow through. This revealed the<br />
high abundance of less hydrophobic proteins in the yeast cell proteome in comparison to the<br />
highly hydrophobic proteins. This also confirmed the less hydrophobic nature of the yeast cell<br />
proteome (104).<br />
Table 11: Standardized chromatographic conditions<br />
Feature<br />
Description<br />
Column volume<br />
5 mL<br />
Flow rate<br />
1 mL / min<br />
Equilibration buffer 10 CV a , 1.6 M, (NH 4 ) 2 SO 4 pH 7.5<br />
Sample load<br />
5 CV a , (8 mg / mL)<br />
Linear Gradient<br />
25 CV a<br />
Fraction volume<br />
3 mL<br />
CV a Column volume<br />
76
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