Thesis final - after defense-7 - Jacobs University

Chapter 3
Ether, some 2-D gels were observed to have the restricted number of protein spots which
could be used as an advantage to have the targeted product on the specific conditions. Overall,
three dimensional separation of the yeast cell proteome was performed, based on
hydrophobicity (HIC), and then followed by isoelectric point and molecular weight (2-D
PAGE). The three dimensional characterization can be a suitable method when choosing an
efficient host for the protein purification (112). The chromatographic methods can be used in
the prefractionation steps for proteomics studies. Several chromatographies have been used
previously to decrease the dynamic range of the cell proteomes on 2-D gels such as heparin
and hydroxyapatite used for the Haemophilus influenza and Escherichia coli, respectively (76,
109). However, it was an initial effort to fractionate the yeast cell proteome by several
hydrophobic adsorbents followed by its proteomics analysis.
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