Thesis final - after defense-7 - Jacobs University

Chapter 3
Table 13. List of the identified proteins fractionated by Sepharose-Phenyl and their characterization with different properties.
Prot A Salt B Prot≠ C Prot. I.D D Mr E pI F PDB G ASH H AH I AH J AH K AP L AP M AF N AB O
Low 1.6 A1-PS KIN4_YEAST 90 9.50 2LAH 0.3864 0.356 0.486 0.570 0.495 0.303 0.540 0.622
Medium
A2-PS ORF194 21 8.50 2X5T 0.4184 0.384 0.497 0.594 0.490 0.321 0.604 0.628
A3-PS YJM789 93 6.05 3A15 0.4245 0.334 0.448 0.566 0.532 0.343 0.579 0.604
A4-PS RPIA_YEAST 28 5.63 3KWM 0.4351 0.408 0.526 0.625 0.465 0.288 0.584 0.630
1.4 B1-PS YHR042Wp 33 8.45 2XNC 0.4194 0.366 0.462 0.559 0.507 0.348 0.602 0.648
B2-PS NDUS6_YEAST 12 9.37 3RHD 0.4202 0.405 0.520 0.600 0.462 0.289 0.608 0.605
B3-PS BIG1_YEAST 39 5.20 3LTL 0.4231 0.368 0.542 0.586 0.440 0.326 0.610 0.694
B4-PS LEUC_YEAST 85 5.61 3Q68 0.4342 0.401 0.492 0.578 0.465 0.285 0.612 0.626
1.0 C1-PS ICP55_YEAST 57 6.11 2XPG 0.4281 0.383 0.495 0.607 0.456 0.273 0.615 0.641
C2-PS PRS7_YEAST 51 5.32 3BPT 0.4351 0.383 0.496 0.594 0.487 0.324 0.612 0.623
C3-PS MSC3_YEAST 80 9.14 2WGP 0.4522 0.401 0.518 0.579 0.517 0.280 0.612 0.601
C4-PS RE114_YEAST 48 9.26 2PMI 0.4478 0.377 0.506 0.578 0.481 0.253 0.629 0.655
0.8 D1-PS PNPP_YEAST 34 6.00 1VJR 0.4473 0.414 0.547 0.621 0.428 0.243 0.601 0.635
D2-PS CDC73_YEAST 44 7.72 3OIP 0.4499 0.415 0.509 0.599 0.476 0.284 0.620 0.641
D3-PS YER145c-a-yeast 16 9.51 3HIE 0.4515 0.418 0.539 0.624 0.454 0.274 0.609 0.653
0.6 E1-PS RS3A1_YEAST 28 10 2KHJ 0.4535 0.415 0.547 0.586 0.482 0.258 0.608 0.649
E2-PS KPYK1_YEAST 54 8.00 1A3X 0.4583 0.411 0.537 0.624 0.449 0.264 0.616 0.645
E3-PS UB4_YEAST 105 8.44 2V6Z 0.4609 0.411 0.539 0.605 0.454 0.249 0.612 0.651
E4-PS VATC_YEAST 44 6.22 1U7l 0.4615 0.415 0.534 0.621 0.451 0.240 0.623 0.667
High 0.2 F1-PS GPN_YEAST 39 4.43 1A4R 0.4647 0.432 0.628 0.628 0.454 0.237 0.624 0.654
F2-PS METE_YEAST 85 6.07 3EG3 0.4757 0.410 0.534 0.621 0.452 0.234 0.632 0.655
F3-PS ADH1_YEAST 36 6.21 3MEQ 0.4643 0.426 0.560 0.633 0.426 0.227 0.639 0.604
0 G1-PS CEX1_YEAST 84 5.27 1Z3H 0.4795 0.418 0.540 0.621 0.446 0.242 0.617 0.655
G2-PS DPM1_YEAST 30 7.70 2GEJ 0.4997 0.452 0.572 0.635 0.411 0.245 0.640 0.666
G3-PS 54S L22 34 9.98 3R3T 0.5488 0.449 0.660 0.655 0.412 0.298 0.639 0.635
G4-PS MCM3_YEAST 107 5.26 3F9V 0.5853 0.455 0.628 0.603 0.410 0.214 0.624 0.628
A ; Protein hydrophobicity, B ; Salt concentration, C ; Identity number, D ; I.D. in SwissProt database, E ; Molecular weight, F ; Isoelectric point, G ; I.D in
PDB databank, H ; Average surface hydrophobicity calculated by Cowan-Whittaker’s scale, I, J and K ; Average hydrophobicity calculated by the scales
of Miyazawa-Jernigan, Cowan-Whittaker and Tanford, respectively. L and M ; Average polarity calculated by the scales of Grantham and Zimmerman,
respectively. N ; Average flexibility calculated by Bhaskaran and Ponnuswamy’s scale, O ; Average bulkiness calculated by Zimmerman’s scale.
83