Thesis final - after defense-7 - Jacobs University

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Chapter 1 • To uncover for the first time the influence of different base supports in HIC with a proteome wide approach. • To explore the physicochemical properties of proteins for their correlation with chromatographic separation and ability to differentiate among different ligands and base supports. • To reconnoitre the contaminant profile of the yeast cell proteome <strong>after</strong> chromatographic fractionation using 2-D PAGE, mass spectrometry and bioinformatics. • To collect the experimental data and define certain operational windows, which can be annotated for the future implementation of the in silico downstream processing. 22
Chapter 2 EXPERIMENTAL APPROACH
Page 1 and 2: A proteome wide approach to underst
Page 3 and 4: I also want to mention the efforts
Page 5 and 6: Dedication To my father Haji Sher A
Page 7 and 8: Table of Contents 1. Introduction .
Page 9 and 10: 3.3.2.2. Protein distributions on 2
Page 11 and 12: Chapter 1 1. Introduction 1.1. Intr
Page 13 and 14: Chapter 1 cell proteome to facilita
Page 15 and 16: Chapter 1 proteins. It is this diff
Page 17 and 18: Chapter 1 Salt conc. decreasing (Gr
Page 19 and 20: Chapter 1 The ions at the start of
Page 21 and 22: Chapter 1 exposure of hydrophobic r
Page 23 and 24: Chapter 1 based on the surface hydr
Page 25 and 26: Chapter 1 1.5. Analysis of the yeas
Page 27 and 28: Chapter 1 molecular weight impuriti
Page 29 and 30: Chapter 1 followed by its introduct
Page 31: Chapter 1 protein towards purificat
Page 35 and 36: Chapter 2 Yeast cell proteome and c
Page 37 and 38: Chapter 2 ligands (Toyopearl-Hexyl,
Page 39 and 40: Chapter 2 number of spots was count
Page 41 and 42: Chapter 2 (http://www.matrixscience
Page 43 and 44: Chapter 3 RESULTS AND DISCUSSION
Page 45 and 46: Chapter 3 to the retention behavior
Page 47 and 48: Chapter 3 investigate the hydrophob
Page 49 and 50: Chapter 3 the interior of the prote
Page 51 and 52: Chapter 3 A B C Figure 9: The cryst
Page 53 and 54: Chapter 3 of the comparative analys
Page 55 and 56: Chapter 3 Figure 11: Represent the
Page 57 and 58: Chapter 3 Ether, some 2-D gels were
Page 59 and 60: Chapter 3 Figure 13: Represent 2-D
Page 61 and 62: Chapter 3 Table 5. List of the iden
Page 63 and 64: Chapter 3 Table 7. List of the iden
Page 65 and 66: Chapter 3 amino acids by Cowan-Whit
Page 67 and 68: Chapter 3 Figure 15 C: Represents t
Page 69 and 70: Chapter 3 Figure 16 A: Represents t
Page 71 and 72: Chapter 3 The polarity value for th
Page 73 and 74: Chapter 3 Figure 17: Represents the
Page 75 and 76: Chapter 3 Figure 18: Represents the
Page 77 and 78: Chapter 3 published papers with mod
Page 79 and 80: Chapter 3 ligands revealed less dif
Page 81 and 82: Chapter 3 3.2. Influence of the dif
Page 83 and 84:
Chapter 3 Table 10: The chemistry o
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Chapter 3 where approximately 50% o
Page 87 and 88:
Chapter 3 3.2.3.2. The electrophore
Page 89 and 90:
Chapter 3 Figure 22: Represent 2-D
Page 91 and 92:
Chapter 3 Table 12. List of the ide
Page 93 and 94:
Chapter 3 Table 14. List of the ide
Page 95 and 96:
Chapter 3 r 2 values from the data
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Chapter 3 Figure 24 C: Represents t
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Chapter 3 approximately 47%. The me
Page 101 and 102:
Chapter 3 Figure 25 B: Represents t
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Chapter 3 values have been reported
Page 105 and 106:
Chapter 3 towards conformational ch
Page 107 and 108:
Chapter 3 Figure 28: Represents the
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Chapter 3 among the adsorbents were
Page 111 and 112:
Chapter 3 3.3. Protein distribution
Page 113 and 114:
Chapter 3 3.3.2.2. Protein distribu
Page 115 and 116:
Chapter 3 Figure 29 B: Represent th
Page 117 and 118:
Chapter 3 When the number of smalle
Page 119 and 120:
Chapter 3 and hydrophobic interacti
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Chapter 4 CONCLUSIONS
Page 123 and 124:
Chapter 4 • The base supports rev
Page 125 and 126:
Chapter 4 additional understanding
Page 127 and 128:
References 13. Lienqueo, M.E.; Lese
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References 32. Salgado, J.C.; Rapap
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References 52. B.H.J., H. Accessibl
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References 71. Xindu, G. and Regnie
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References 91. Alonso-Orgaz, S.; Mo
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References 112. Gu, Z. and Glatz, C
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References 132. Reubsaet, J.L.E. an
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Thesis final - after defense-7 - Jacobs University

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