Thesis final - after defense-7 - Jacobs University

Chapter 3
3.2.2. Chemistry of the base supports
The adsorbents such as Toyopearl-Phenyl, Sepharose-Phenyl and Source-Phenyl were used
with different base supports harbored phenyl as a common ligand. The physical properties of
these adsorbents have been given in Table 9 as provided by the Vendor Company (99). The
commercial adsorbents are usually composed of ligands and base supports of various nature.
There are several base supports usually utilized in HIC such as Sepaharose ® , Toyopearl ® and
Source ®
that are composed of certain polymers in backbone chemistries i.e agarose,
methacrylate and polystyrene. These polymers are usually of hydrophobic nature but were
modified to the hydrophilic nature by coupling to certain ether and hydroxyl groups (99). The
same was the case with polystyrene based adsorbents, however its chemistry has not reported
yet. The chemistry of the three mentioned adsorbents has been shown in Table 10.
Table 9: Physical properties of the adsorbents with different base support (99)
Adsorbents Base supports Ligands Bead size
(µm)
Pore size
(nm)
a DBC
(mg/mL)
Toyopearl-
Phenyl
Sepharose-
Phenyl
Toyopearl ® Phenyl 100 100 40
Sepharose ® Phenyl 34 65 35
Source-Phenyl Source ® Phenyl 15 34 25
a DBC; Dynamic binding capacity
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