in vitro PHARMACOLOGY 2011 CATALOG - Cerep
in vitro PHARMACOLOGY 2011 CATALOG - Cerep
in vitro PHARMACOLOGY 2011 CATALOG - Cerep
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115<br />
KDR k<strong>in</strong>ase (VEGFR2)<br />
Ref. 2864<br />
Q 3 weeks<br />
Included <strong>in</strong>:<br />
ExpresS Diversity k<strong>in</strong>ase profile<br />
Comprehensive k<strong>in</strong>ase profile<br />
Source<br />
human recomb<strong>in</strong>ant (Sf9 cells)<br />
Substrate<br />
ATP + Ulight-CAGAGAIETDKEYYTVKD<br />
(100 nM)<br />
Measured product phospho-Ulight-CAGAGAIETDKEYYTVKD<br />
Detection method LANCE<br />
Reference<br />
staurospor<strong>in</strong>e (IC 50 : 3 nM)<br />
Itokawa, T. et al. (2002) Mol. Cancer Ther., 1: 295-302.<br />
prote<strong>in</strong>-tyros<strong>in</strong>e k<strong>in</strong>ases [Rtk] ❚<br />
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<strong>Cerep</strong><br />
services<br />
Receptors<br />
LTK<br />
Ref. 2642<br />
Q 3 weeks<br />
Included <strong>in</strong>:<br />
Comprehensive k<strong>in</strong>ase profile<br />
Source<br />
Substrate<br />
Measured product<br />
Detection method<br />
Reference<br />
human recomb<strong>in</strong>ant<br />
ATP + Ulight-Poly GAT[EAY(1:1:1)]n<br />
(5 nM)<br />
phospho-Ulight-Poly GAT[EAY(1:1:1)]n<br />
LANCE<br />
staurospor<strong>in</strong>e (IC 50 : 5.3 nM)<br />
Ueno, H. et al. (1996) J. Biol. Chem., 271: 27707-27714.<br />
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Ion<br />
channels<br />
Transporters<br />
Mer k<strong>in</strong>ase<br />
Ref. 3075<br />
Q 3 weeks<br />
Source<br />
Substrate<br />
Measured product<br />
Detection method<br />
Reference<br />
human recomb<strong>in</strong>ant<br />
ATP + Ulight-TK peptide<br />
(50 nM)<br />
phospho-Ulight-TK peptide<br />
LANCE<br />
staurospor<strong>in</strong>e (IC 50 : 2.4 nM)<br />
Camenisch, T.D. et al. (1999) J. Immunol., 162: 3498-3503.<br />
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K<strong>in</strong>ases<br />
Epigenetic &<br />
DNA-related<br />
enzymes<br />
MusK<br />
Ref. 2899<br />
Q 3 weeks<br />
Included <strong>in</strong>:<br />
Comprehensive k<strong>in</strong>ase profile<br />
Source<br />
Substrate<br />
Measured product<br />
Detection method<br />
Reference<br />
human recomb<strong>in</strong>ant (Sf9 cells)<br />
ATP + Ulight-CAGAGAIETDKEYYTVKD<br />
(100 nM)<br />
phospho-Ulight-CAGAGAIETDKEYYTVKD<br />
LANCE<br />
staurospor<strong>in</strong>e (IC 50 : 2.4 nM)<br />
Watty, A. and Burden, S.J. (2002) J. Biol. Chem., 277: 50457-50462.<br />
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Other<br />
enzymes<br />
Specialized<br />
cellular<br />
assays<br />
PDGFR<br />
cellul ar<br />
Ref. 3156 Activator effect<br />
Ref. 3160 Inhibitor effect<br />
Q 3 weeks<br />
Source<br />
Measured product<br />
Detection method<br />
Balb/c 3T3 cells<br />
impedance<br />
cellular dielectric spectroscopy<br />
Activator effect Control PDGF-BB (3 nM)<br />
Reference PDGF-BB (EC 50 : 0.044 nM)<br />
Inhibitor effect Stimulant PDGF-BB (0.1 nM)<br />
Reference PDGF <strong>in</strong>hib. IV (IC 50 : 282 nM)<br />
Maldonado, P.E. et al. (1988) J. Membr. Biol., 106: 203-210<br />
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[Solvent] must be kept 0.1%<br />
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Standard<br />
profiles<br />
Test<strong>in</strong>g<br />
conditions<br />
PDGFRa k<strong>in</strong>ase<br />
Ref. 3064<br />
Q 3 weeks<br />
Included <strong>in</strong>:<br />
Comprehensive k<strong>in</strong>ase profile<br />
Source<br />
Substrate<br />
Measured product<br />
Detection method<br />
Reference<br />
human recomb<strong>in</strong>ant<br />
ATP + Ulight-TK peptide<br />
(100 nM)<br />
phospho-Ulight-TK peptide<br />
LANCE<br />
staurospor<strong>in</strong>e (IC 50 : 0.38 nM)<br />
Songyang, Z. et al. (1995) Nature, 373: 536-539.<br />
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Order<strong>in</strong>g<br />
<strong>in</strong>formation<br />
Assay list<br />
& <strong>in</strong>dex
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