in vitro PHARMACOLOGY 2011 CATALOG - Cerep
in vitro PHARMACOLOGY 2011 CATALOG - Cerep
in vitro PHARMACOLOGY 2011 CATALOG - Cerep
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137<br />
❚ prote<strong>in</strong>-SERINE/THREONINE k<strong>in</strong>ases [AGC]<br />
<strong>Cerep</strong><br />
services<br />
Akt1/PKBa<br />
Ref. 2923<br />
Q 3 weeks<br />
Included <strong>in</strong>:<br />
ExpresS Diversity k<strong>in</strong>ase profile<br />
Organ safety profile<br />
Comprehensive k<strong>in</strong>ase profile<br />
Source<br />
human recomb<strong>in</strong>ant (<strong>in</strong>sect cells)<br />
Substrate ATP + CREBtide (CKRREILSRRPSYRK)<br />
(25 nM)<br />
Measured product phospho-CREBtide (CKRREILSRRPSYRK)<br />
Detection method LANCE<br />
Reference<br />
staurospor<strong>in</strong>e (IC 50 : 37 nM)<br />
Barnett, S.F. et al. (2005) Biochem. J., 385: 399-408.<br />
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Receptors<br />
Ion<br />
channels<br />
Akt2/PKBb<br />
Ref. 2924<br />
Q 3 weeks<br />
Included <strong>in</strong>:<br />
Comprehensive k<strong>in</strong>ase profile<br />
Source<br />
human recomb<strong>in</strong>ant<br />
Substrate ATP + Ulight-RRRSLLE<br />
(50 nM)<br />
Measured product phospho-Ulight-RRRSLLE<br />
Detection method LANCE<br />
Reference<br />
staurospor<strong>in</strong>e (IC 50 : 11.5 nM)<br />
Barnett, S.F. et al. (2005) Biochem. J., 385: 399-408.<br />
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Transporters<br />
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K<strong>in</strong>ases<br />
Akt3/PKBg<br />
Ref. 2925<br />
Q 3 weeks<br />
Included <strong>in</strong>:<br />
Comprehensive k<strong>in</strong>ase profile<br />
Source<br />
human recomb<strong>in</strong>ant<br />
Substrate ATP + Ulight-RRRSLLE<br />
(50 nM)<br />
Measured product phospho-Ulight-RRRSLLE<br />
Detection method LANCE<br />
Reference<br />
staurospor<strong>in</strong>e (IC 50 : 13 nM)<br />
Barnett, S.F. et al. (2005) Biochem. J., 385: 399-408.<br />
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Epigenetic &<br />
DNA-related<br />
enzymes<br />
Other<br />
enzymes<br />
Akt1/2<br />
cellul ar<br />
Ref. 3099 Activator effect<br />
Ref. 3104 Inhibitor effect<br />
Q 3 weeks<br />
Source<br />
A431 cells<br />
Measured product phosphorylation<br />
Detection method AlphaScreen<br />
Activator effect Control EGF (1 µM)<br />
Reference EGF (EC 50 : 1 nM)<br />
Inhibitor effect Stimulant EGF (10 nM)<br />
Reference wortmann<strong>in</strong> (IC 50 : 23 nM)<br />
Kim, S.H. et al.(2009) Ann. N. Y. Acad. Sci., 1171: 642-648.<br />
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[Solvent] must be kept 0.1%<br />
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Specialized<br />
cellular<br />
assays<br />
Standard<br />
profiles<br />
CRIK<br />
Ref. 2628<br />
Q 3 weeks<br />
Included <strong>in</strong>:<br />
Comprehensive k<strong>in</strong>ase profile<br />
Source<br />
human recomb<strong>in</strong>ant<br />
Substrate ATP + Ulight-ARTKQTARKSTGGKAPRKQLAGCG<br />
(75 nM)<br />
Measured product phospho-Ulight-ARTKQTARKSTGKAPRKQL<br />
AGCG<br />
Detection method LANCE<br />
Reference staurospor<strong>in</strong>e (IC 50 : 40 nM)<br />
Zhao, Z.S. and Manser, E. (2005) Biochem. J., 386: 201-214.<br />
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Test<strong>in</strong>g<br />
conditions<br />
Order<strong>in</strong>g<br />
<strong>in</strong>formation<br />
For further details and updated <strong>in</strong>formation on assays:<br />
❚ Please go to www.cerep.com catalog onl<strong>in</strong>e or contact us at sales@cerep.com<br />
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❚ Europe: +33 (0)5 49 89 30 00 – USA: +1 (425) 895 8666 – Japan: +81 (0)3 3354 4026 – Ch<strong>in</strong>a: +86 21 5132 0568<br />
Assay developed <strong>in</strong> 2010 New assay conditions and/or assay converted from HTRF ® to LANCEUltra ®<br />
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<br />
<br />
Human Q Standard turnaround time<br />
Assay list<br />
& <strong>in</strong>dex
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