in vitro PHARMACOLOGY 2011 CATALOG - Cerep
in vitro PHARMACOLOGY 2011 CATALOG - Cerep
in vitro PHARMACOLOGY 2011 CATALOG - Cerep
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190 <strong>in</strong> <strong>vitro</strong> pharmacology <strong>2011</strong> catalog<br />
❚ Free radical secretion<br />
superoxide O 2<br />
–<br />
secretion<br />
cellul ar<br />
Source<br />
Substrate<br />
Stimulant<br />
Measured product<br />
Detection method<br />
differentiated HL-60 cells<br />
cytochrome C (19 µM)<br />
PMA (30 nM)<br />
-<br />
O 2 (cytochrome C reduction)<br />
photometry<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
Ref. 0275<br />
[CUSTOM OFFER]<br />
Reference<br />
diphenyleneiodonium (IC 50 : 380 nM)<br />
Lorico, A. et al. (1986) Biochem. Pharmacol., 35: 2443-2445.<br />
<br />
<br />
H 2 O 2 secretion<br />
cellul ar<br />
Ref. 0277<br />
[CUSTOM OFFER]<br />
Source<br />
differentiated HL-60 cells<br />
Substrate<br />
scopolet<strong>in</strong> (4 µM)<br />
Stimulant<br />
PMA (19 nM)<br />
Measured product H 2 O 2 (scopolet<strong>in</strong> oxidation)<br />
Detection method fluorimetry<br />
Reference<br />
catalase (IC 50 : 220 U/mL)<br />
Root, R.K. et al. (1975) J. Cl<strong>in</strong>. Invest., 55: 945-955.<br />
H 2O2 secretion (% of control)<br />
100<br />
50<br />
0<br />
1 10 100 1000 10000<br />
log [drug] (U/mL)<br />
catalase<br />
H 2 O 2 scaveng<strong>in</strong>g<br />
cellul ar<br />
Ref. 0278<br />
[CUSTOM OFFER]<br />
Source<br />
cell free system<br />
Substrate<br />
scopolet<strong>in</strong> (4 µM)<br />
Stimulant<br />
H 2 O 2 (2 µM)<br />
Measured product scopolet<strong>in</strong> oxidation<br />
Detection method fluorimetry<br />
Reference<br />
catalase (IC 50 : 27 U/mL)<br />
Root, R.K. et al. (1975) J. Cl<strong>in</strong>. Invest., 55: 945-955.<br />
H 2 O 2 scaveng<strong>in</strong>g (% of control)<br />
100<br />
50<br />
0<br />
0 1 10 100 1000<br />
log [drug] (U/mL)<br />
catalase<br />
lipid peroxidation<br />
cellul ar<br />
Ref. 0279<br />
[CUSTOM OFFER]<br />
Source<br />
rat liver microsomes<br />
Substrate<br />
ascorbic acid (0.1 mM)<br />
Measured product malonaldehyde<br />
Detection method photometry<br />
Reference<br />
N-propyl gallate (IC 50 : 2.4 µM)<br />
Aruoma, O.I. et al. (1990) Free Rad. Res. Commun., 10: 143-157.<br />
malonaldehyde production (% of control)<br />
100<br />
50<br />
0<br />
-7 -6 -5 -4<br />
log [drug] (M)<br />
N-propyl gallate<br />
ebselen<br />
trolox<br />
❚ Cell adhesion<br />
expression of endothelial adhesion prote<strong>in</strong>s (ICAM1/VCAM-1)<br />
cellul ar<br />
Ref. 0244<br />
[CUSTOM OFFER]<br />
Source<br />
rest<strong>in</strong>g U-937 cells to stimulated HUVEC<br />
Stimulant<br />
LPS (1 µg/mL)<br />
Measured product ICAM1/VCAM-1 mediated adhesion<br />
Detection method fluorimetry<br />
Reference<br />
NDGA (IC 50 : 10 µM)<br />
[Solvent] must be kept ≤ 0.3%<br />
Staunton, D.E. et al. (1989) Nature, 339: 61-64.
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