in vitro PHARMACOLOGY 2011 CATALOG - Cerep
in vitro PHARMACOLOGY 2011 CATALOG - Cerep
in vitro PHARMACOLOGY 2011 CATALOG - Cerep
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183<br />
miscellaneous enzymes ❚<br />
carbonic anhydrase II<br />
Ref. 2572<br />
Q 3 weeks<br />
Source<br />
human erythrocytes<br />
Substrate<br />
4-nitrophenyl acetate (450 µM)<br />
Measured product 4-nitrophenol<br />
Detection method photometry<br />
Reference<br />
acetazolamide (IC 50 : 29 nM)<br />
Iyer, R. et al. (2006) J. Biomol. Screen., 11: 782-791.<br />
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<strong>Cerep</strong><br />
services<br />
Receptors<br />
FAAH (fatty acid amide hydrolase)<br />
Source<br />
human recomb<strong>in</strong>ant (Sf21 cells)<br />
Substrate<br />
AMC arachidonoyl amide (20 µM)<br />
Measured product 7-am<strong>in</strong>o-4-methyl coumar<strong>in</strong><br />
Detection method Fluorimetry<br />
Ref. 2960<br />
Reference<br />
URB597 (IC 50 :90 nM)<br />
Q 3 weeks<br />
Vandevoorde, S. (2008) Curr. Top. Med. Chem., 8: 247-267.<br />
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Ion<br />
channels<br />
Transporters<br />
HMG-CoA reductase<br />
Ref. 0187<br />
Q 6 weeks<br />
Source<br />
Syrian hamster recomb<strong>in</strong>ant (E. coli)<br />
Substrate<br />
Measured product<br />
Detection method<br />
Reference<br />
HMG-CoA (130 µM) <br />
mevalonate<br />
photometry<br />
mevastat<strong>in</strong> (IC 50 : 4.7 µM)<br />
Omhumar, R.V. et al. (1994) J. Biol. Chem., 269: 6810-6814.<br />
enzyme activity (% of control)<br />
100<br />
50<br />
0<br />
-7 -6 -5 -4<br />
log [drug] (M)<br />
<br />
mevastat<strong>in</strong><br />
K<strong>in</strong>ases<br />
Epigenetic &<br />
DNA-related<br />
enzymes<br />
myeloperoxidase<br />
Ref. 0193<br />
Q <strong>in</strong>quire<br />
Source<br />
human leukocytes<br />
Substrate<br />
guaiacol (80 mM)<br />
Measured product tetraguaiacol<br />
Detection method photometry<br />
Reference<br />
NDGA (IC 50 : 5.1 µM)<br />
Desser, R.K. et al. (1972) Arch. Biochem. Biophys., 148: 452-465.<br />
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-10 -9 -8 -7 -6<br />
-10 -9 -6 -8 -7 -5 -4<br />
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-11 -10 -9 -8 -7 -6<br />
-12 -11 -10 -6<br />
-9 -8 -7<br />
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100<br />
0<br />
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Other<br />
enzymes<br />
Specialized<br />
cellular<br />
assays<br />
tubul<strong>in</strong><br />
Ref. 3274<br />
Q 3 weeks<br />
Source<br />
porc<strong>in</strong>e bra<strong>in</strong><br />
Substrate<br />
GTP (1 mM)<br />
Measured comp. microtubules polymerisation<br />
Detection method fluorimetry<br />
Reference<br />
v<strong>in</strong>blast<strong>in</strong>e (IC 50 :1200 nM)<br />
Bonne, D et al.(1985) J. Biol. Chem., 260: 2819-2825.<br />
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Standard<br />
profiles<br />
Test<strong>in</strong>g<br />
conditions<br />
xanth<strong>in</strong>e oxidase/superoxide O 2<br />
–<br />
scaveng<strong>in</strong>g<br />
Ref. 0276<br />
Q 3 weeks<br />
Included <strong>in</strong>:<br />
BioPr<strong>in</strong>t ® profile<br />
Source<br />
purified xanth<strong>in</strong>e oxidase from bov<strong>in</strong>e milk<br />
Substrate<br />
Measured product<br />
Detection method<br />
Reference<br />
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hypoxanth<strong>in</strong>e (10 µM)<br />
O 2<br />
–<br />
+ uric acid<br />
photometry<br />
allopur<strong>in</strong>ol (IC 50 : 2.37 µM)<br />
McCord, J.K. and Fridovich, I. (1969) J. Biol. Chem., 244: 6049-6055.<br />
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Order<strong>in</strong>g<br />
<strong>in</strong>formation<br />
Assay list<br />
& <strong>in</strong>dex
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