in vitro PHARMACOLOGY 2011 CATALOG - Cerep

191
expression of endothelial adhesion proteins (ELAM-1)
cellul ar
Ref. 0245
[CUSTOM OFFER]
Source
Stimulant
Measured product
Detection method
Reference
[Solvent] must be kept ≤ 0.1%
resting HL-60 cells to stimulated HUVEC
TNF-α (3 ng/mL)
ELAM-1 mediated adhesion
fluorimetry
NDGA (IC 50 : 18 µM)
Vaporciyan, A.A. et al. (1993) J. Immunol. Met., 159: 93-100.
expression of endothelial adhesion proteins (VCAM-1)
cellul ar
Ref. 0246
[CUSTOM OFFER]
Source
resting Ramos cells to stimulated HUVEC
Stimulant
TNF-α (1 ng/mL) + IL-4 (500 U/mL)
Measured product VCAM-1 mediated adhesion
Detection method fluorimetry
Reference
cycloheximide (IC 50 : 2 µM)
[Solvent] must be kept ≤ 0.1%
Kapiotis, S. et al. (1994) Circulatory Shock, 43: 18-25.
activation of monocyte adhesion proteins (U-937/HUVEC)
cellul ar
Ref. 0247
[CUSTOM OFFER]
Source
stimulated U-937 cells to resting HUVEC
Stimulant
PMA (100 nM)
Measured product activation of U-937 adhesion molecules
Detection method fluorimetry
Reference
staurosporine (IC 50 : 230 nM)
[Solvent] must be kept ≤ 0.1%
Cavender, D.E. et al. (1991) J. Leuk. Biol., 49: 566-578.
adhesion (% of control)
adhesion (% of control)
cell adhesion ❚
100
50
cycloheximide
0
-7 -6 -5
log [drug] (M)
100
50
0
staurosporine
-8 -7 -6
log [drug] (M)
Cerep
services
Receptors
Ion
channels
Transporters
Kinases
Epigenetic &
DNA-related
enzymes
activation of monocyte adhesion proteins (matrix proteins)
cellul ar
Ref. 0249
[CUSTOM OFFER]
Source
stimulated U-937 cells to extracellular
matrix proteins (collagen + fibronectin)
Stimulant
PMA (100 nM)
Measured product activation of U-937 adhesion molecules
Detection method fluorimetry
Reference staurosporine (IC 50 : 160 nM)
[Solvent] must be kept ≤ 0.1%
Cavender, D.E. et al. (1991) J. Leuk. Biol., 49: 566-578.
adhesion (% of control)
100
50
0
-8 -7 -6 -5
log [drug] (M)
staurosporine
Other
enzymes
Specialized
cellular
assays
activation of granulocyte adhesion proteins (HL-60/HUVEC)
cellul ar
Ref. 0248
[CUSTOM OFFER]
Source
stimulated HL-60 cells to resting HUVEC
Stimulant
PMA (100 nM)
Measured product activation of HL-60 adhesion molecules
Detection method fluorimetry
Reference
staurosporine (IC 50 : 540 nM)
[Solvent] must be kept ≤ 0.1%
Cavender, D.E. et al. (1991) J. Leuk. Biol., 49: 566-578.
adhesion (% of control)
100
50
0
-8 -7 -6
log [drug] (M)
staurosporine
Standard
profiles
Testing
conditions
Ordering
information
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Assay list
& index