in vitro PHARMACOLOGY 2011 CATALOG - Cerep
in vitro PHARMACOLOGY 2011 CATALOG - Cerep
in vitro PHARMACOLOGY 2011 CATALOG - Cerep
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164 <strong>in</strong> <strong>vitro</strong> pharmacology <strong>2011</strong> catalog<br />
❚ phosphatases<br />
phosphatase 2B<br />
Source<br />
Substrate<br />
Measured product<br />
Detection method<br />
bov<strong>in</strong>e bra<strong>in</strong><br />
pNPP (10 mM)<br />
pNP<br />
photometry<br />
enzyme activity (% of control)<br />
100<br />
50<br />
Ref. 0365<br />
Q 3 weeks<br />
Reference<br />
trifluoperaz<strong>in</strong>e (IC 50 : 37 µM)<br />
Pallen, C.J. and Wang, J.H. (1984) J. Biol. Chem., 259: 6134-6141.<br />
0<br />
trifluoperaz<strong>in</strong>e<br />
-7 -6 -5 -4 -3<br />
log [drug] (M)<br />
phosphatase CD45<br />
Ref. 2594<br />
Q 3 weeks<br />
Source<br />
human recomb<strong>in</strong>ant (yeast)<br />
Substrate<br />
DIFMUP (100 µM)<br />
Measured product DIFMU<br />
Detection method fluorimetry<br />
Reference<br />
ammonium molybdate (IC 50 : 60 nM)<br />
Welte, S. et al. (2005) Anal. Biochem., 338: 32-38.<br />
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phosphatase CDC25A<br />
Ref. 2561<br />
Q 3 weeks<br />
Included <strong>in</strong>:<br />
Non-k<strong>in</strong>ase enzyme profile<br />
Diversity profile<br />
Organ safety profile<br />
Source<br />
Substrate<br />
Measured product<br />
Detection method<br />
Reference<br />
human recomb<strong>in</strong>ant (E. coli)<br />
OMFP (20 µM)<br />
OMF<br />
fluorimetry<br />
NSC 663284 (IC 50 : 350 nM)<br />
Tierno, M.B. et al. (2007) Nat. Protoc., 2: 1134-1144.<br />
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phosphatase CDC25B<br />
Source<br />
human recomb<strong>in</strong>ant (E. coli)<br />
Substrate<br />
OMFP (20 µM)<br />
Measured product OMF<br />
Detection method fluorimetry<br />
Ref. 2541<br />
Reference<br />
NSC 663284 (IC 50 : 500 nM)<br />
Q 3 weeks<br />
Tierno, M.B. et al. (2007) Nat. Protoc., 2: 1134-1144.<br />
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phosphatase CDC25C<br />
Source<br />
human recomb<strong>in</strong>ant (E. coli)<br />
Substrate<br />
OMFP (20 µM)<br />
Measured product OMF<br />
Detection method fluorimetry<br />
Ref. 2562<br />
Reference<br />
NSC 663284 (IC 50 : 410 nM)<br />
Q 3 weeks<br />
Tierno, M.B. et al. (2007) Nat. Protoc., 2: 1134-1144.<br />
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phosphatase DEP1 (PTPRJ)<br />
Source<br />
human recomb<strong>in</strong>ant (E. coli)<br />
Substrate<br />
DIFMUP (150 µM)<br />
Measured product DIFMU<br />
Detection method fluorimetry<br />
Ref. 2801<br />
Reference<br />
ammonium molybdate (IC 50 : 240 nM)<br />
Q 3 weeks<br />
Welte, S. et al. (2005) Anal. biochem., 338: 32-38.
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