Research Profile - College of Medicine and Health Science - United ...

Department of Pathology
Histopathology/Lymphoma/Breast
Pathology/CNS pathology/Renal Pathology/
Molecular Pathology (Dr. Suhail Al-Salam)
1. Expression of EBV in Hodgkin Lymphoma
among UAE nationals
The epidemiology of Hodgkin’s lymphoma (HL)
shows wide geographic variation in histological
subtypes and in its association with the Epstein-
Barr virus (EBV). The proportion of EBV positive
HL is low in industrialized countries, high in
non-industrialized countries and intermediate
in early-industrialized countries. Reports from
the Arabian Gulf and Middle East are few. The
aim of our study was to determine the
epidemiology of HL in The population of United
Arab Emirates (UAE) nationals, an early
industrialized country in the Arabian Gulf, and
to delineate the extent of its association with
EBV. We review the cases of HL for the period
1988 through 2004 for histological classification
and demographic data. All Cases diagnosed as
Hodgkin’s lymphoma were examined for the
presence of EBV using immunohistochemistry
(IHC) for the latent membrane protein I (LMPI)
and in-situ hybridization (ISH) for EBV encoded
RNA (EBER) to determine the prevalence of EBV
in Hodgkin cells and its possible role in the
Figure: (A) Hodgkin lymphoma with classical Reed–Sternberg cell showing dark-purple nuclear staining for EBER, in situ
hybridization, X400. (B) Classical Reed–Sternberg cell showing membranous, cytoplasmic and Golgi immunoreactivity to LMP-1,
streptavidin–biotin, X400
56
pathogenesis of Hodgkin’s lymphoma. EBV was
seen in 17 of 45 (38%) cases of HL and was
predominately seen in the MC subtype followed
by NS, LD and LR subtypes, respectively.
2. Types of mutations in BRCA 1 and BRCA 2
genes in breast carcinoma among United Arab
Emirates nationals and their correlation with
P53, Ki 67, bcl2, nm2, c-erb-B2, estrogen receptors
and progesterone receptors expression.
Breast carcinoma is the most common
malignant tumor and the leading cause of
cancer death in women all over the world. It is
the commonest cancer among UAE population
and the most common cancer among females
The age specific incidence ratio for females in
UAE was 19.4 per 100,000 population. It is
currently estimated that 5–10% of all breast
cancers are hereditary and attributable to
mutations in several high penetrance
susceptibility genes, of which only two have
been identified BRCA1 and BRCA2. Earlier
estimates suggested that BRCA1 and BRCA2
mutations were responsible for 75% of sitespecific
breast cancer families and the majority
of breast and ovarian cancer families. The aim
of this study is to identify the types of mutations
in BRCA 1 and BRCA 2 genes in breast carcinoma