Golden Nematode Program Manual - Phytosanitary Resources

Procedures: Preparation, Disinfection, and Clean-up
Survey Site Clean-up
Survey Site Clean-up
After the survey is finished for each collection, then the crew leader
and survey crew will clean-up and disinfect at the survey site.
Crew Leader Responsibilities
After the survey is finished, the crew leader will make sure the
supplies are cleaned and disinfected as follows:
1. Set up three wash tubs for cleaning and sanitizing boots, mesh
bags, and trowels.
A. Prepare a bleach solution (1 part 5.25% bleach (hypochlorite)
to 10 parts of water) in one wash tub to use for sanitizing.
B. Add clear water in the other two tubs to use for cleaning and
rinsing before sanitizing.
2. Remove your boots and have each person remove their boots at
the vehicle, and then have each person clean their boots. See
Cleaning and Disinfecting Materials and Supplies on page
2-2-19.
3. Do not allow anyone to re-enter the field after their boots have
been removed.
4. Clean and disinfect survey tractors and pickup trucks at the
survey site. See Cleaning Vehicles on page 2-2-17.
5. If a van is used to transport samples, then stop at a car wash to
wash the van on the way back to the lab from the survey site.
Manual Survey Crew Responsibilities
After completing a manual survey, the field crew will do as follows:
1. Clean boots, trowels, mesh bags/backpacks with the bleach
solution. See Cleaning and Disinfecting Materials and
Supplies.
2. Confirm boots, trowels and mesh bags have been thoroughly
cleaned with the bleach solution before placing the trowels and
boots in a clean container in transport vehicle.
3. Empty and rinse the buckets, containers, and brushes; and then
clean and disinfect with bleach solution.
Empty wash water onto the same side of the road as the field that was
surveyed, so that the wash water will drain back into the survey area.
4. Place disinfected buckets, containers, and brushes back into the
transport vehicle.
2-2-16 Golden Nematode Program Manual 07/2008-01
PPQ