Golden Nematode Program Manual - Phytosanitary Resources

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Procedures: Laboratory, Rack Room, and Wash Room Sample Wash Room Step 6: Wash the Muck Soil Samples The steps below are for washing muck soil samples only. Washing muck soil samples is much more time-consuming due to the extremely dry nature of the soil. Be extremely careful when processing muck soil samples. Muck soil can resist wetting. Important Wash muck samples as follows: 1. Wear a pollen mask, respirator, and rubber apron. 2. Turn the low water on so there will be a little water in the tank. See the large tank on the right in Figure 2-4-8 on page 2-15. 3. Open the soil sample bag carefully; slowly dump the soil into the Fenwick can; and keep the water on low while dumping the sample. 4. Throw the empty sample bag into the trash can. 5. Use the high-pressure water hose and slowly break up the soil. 6. Allow the low water level to flow just up to the lip of the tank; this brings the soil up and gets everything to the top of the tank. 7. As the debris comes up to the top of the tank, carefully hose and skim the larger debris from the tank into the top No. 20 sieve. See Figure 2-4-9 on page 2-16. 8. As the larger debris is skimmed off with the running water, any golden nematode cysts that may be in the debris will float and come right up to the top (you cannot see the cysts with the naked eye). Gently skim off the smaller debris into the first No. 20 sieve. 9. Thoroughly hose down the top screen and sieve sides to force remaining flotsam in the top sieve down into the lower (bottom) No. 60 sieve. 10. Thoroughly hose the bottom screen to force all the material (flotsam and cysts) from the bottom sieve into the sample numbered beaker. 11. Take the hose and add clear water to the beaker until the water level is about 2" the top. 12. Remove the bottom screen from its holder; thoroughly clean; and return to its holder. 13. Rinse your hands clean between every sample. 2-4-18 Golden Nematode Program Manual 11/2007-01 PPQ
Procedures: Laboratory, Rack Room, and Wash Room Sample Wash Room 14. Prepare to start the next sample. After each collection is washed, sanitize the Fenwick cans and sieves before starting a new collection. Important 11/2007-01 Golden Nematode Program Manual 2-4-19 PPQ
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United States Department of Agricul
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Contents 1 Golden Nematode Program
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List of Figures 1 Golden Nematode P
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List of Figures FIGURE A-1-14 Exam
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List of Tables 1 Golden Nematode Pr
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1 Introduction Golden Nematode Prog
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Introduction Users Users This manua
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Introduction Conventions Convention
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Introduction Conventions subordinat
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2 Procedures Golden Nematode Progra
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Procedures: Introduction Map of Qua
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Procedures: Introduction General Pe
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Procedures: Introduction General Pe
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2 Procedures Golden Nematode Progra
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Procedures: Preparation, Disinfecti
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Page 63 and 64: Procedures: Survey Surveys Outside
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Procedures: Control Non-chemical Co
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A Appendix Golden Nematode Program
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Appendix A: Forms and Worksheets Go
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Appendix A: Forms and Worksheets Sa
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Appendix A: Forms and Worksheets We
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Appendix A: Forms and Worksheets PP
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B Appendix Golden Nematode Program
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Appendix B: Fumigant Monitoring B.
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Appendix B: First Aid Rescue Breat
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C Appendix Golden Nematode Program
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Appendix C: Maintenance of the Manu
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Appendix C: Maintenance of the Manu
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Glossary 5 Golden Nematode Program
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Glossary: Definitions, Terms, and
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Index 1 C Canada 3-1-4 Carriers 2-2
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G Globodera rostochiensis (golden n
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Poisoning signs and symptoms B-1-6
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Trucks 3-1-17, 3-1-30 Tubers 3-1-3
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Golden Nematode Program Manual - Phytosanitary Resources

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