Golden Nematode Program Manual - Phytosanitary Resources

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Procedures: Laboratory, Rack Room, and Wash Room Sample Wash Room FIGURE 2-4-9 Sieves and Skimming Debris 8. As the larger debris is skimmed off with the running water, the golden nematode cysts will float and come right up to the top (you cannot see the cysts with the naked eye). Gently skim off smaller debris (which may contain suspect nematode cysts) into the top No. 20 sieve. 9. Thoroughly hose down the top screen and sieve sides to force everything remaining in the top sieve down into the lower No. 60 sieve (bottom screen). This forces the smaller material down onto the bottom sieve. 10. Each sample must process for a minimum of two minutes. At this time you can start a second sample in the other can. Do not mix the contents of the sample bags together. Important 11. Thoroughly hose the bottom screen and force all material (flotsam and cysts) from the bottom sieve into a beaker. Keep adding water to the screen until the water level is about 2 inches from the beaker’s top. 12. Remove the top screen from its holder and thoroughly clean the screen, then replace in its holder. 13. Forcefully spray through the top screen into the bottom screen to rinse all murkiness from the bottom (water running out of the bottom screen should run clear) 14. Take the hose and add clear water into the beaker until the water and flotsam is about 2" from the top. 15. Thoroughly clean the bottom screen, then return to its holder. 2-4-16 Golden Nematode Program Manual 11/2007-01 PPQ
Procedures: Laboratory, Rack Room, and Wash Room Sample Wash Room 16. Rinse your hands clean between every sample. 17. Prepare to start the next sample. When processing mineral soil samples only, you can wash one sample and then start another in the second tank. While the first tank is filling with water, get a second sample bag and begin processing in the second tank. Do not mix the contents of sample bags together. 11/2007-01 Golden Nematode Program Manual 2-4-17 PPQ
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United States Department of Agricul
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Contents 1 Golden Nematode Program
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List of Figures 1 Golden Nematode P
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List of Figures FIGURE A-1-14 Exam
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List of Tables 1 Golden Nematode Pr
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1 Introduction Golden Nematode Prog
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Introduction Users Users This manua
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Introduction Conventions Convention
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Introduction Conventions subordinat
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2 Procedures Golden Nematode Progra
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Procedures: Introduction Map of Qua
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Procedures: Introduction General Pe
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Procedures: Introduction General Pe
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2 Procedures Golden Nematode Progra
Page 29 and 30: Procedures: Preparation, Disinfecti
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Page 53 and 54: Procedures: Survey Soil Surveys Soi
Page 55 and 56: Procedures: Survey Systematic Manua
Page 57 and 58: Procedures: Survey Mechanical (Whee
Page 59 and 60: Procedures: Survey Selected Area So
Page 61 and 62: Procedures: Survey Survey Times and
Page 63 and 64: Procedures: Survey Surveys Outside
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Procedures: Control Non-chemical Co
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Procedures: Control Non-chemical Co
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A Appendix Golden Nematode Program
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Appendix A: Forms and Worksheets Go
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Appendix A: Forms and Worksheets Sa
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Appendix A: Forms and Worksheets We
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Appendix A: Forms and Worksheets PP
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B Appendix Golden Nematode Program
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Appendix B: Fumigant Monitoring B.
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Appendix B: First Aid Rescue Breat
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C Appendix Golden Nematode Program
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Appendix C: Maintenance of the Manu
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Appendix C: Maintenance of the Manu
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Glossary 5 Golden Nematode Program
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Glossary: Definitions, Terms, and
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Index 1 C Canada 3-1-4 Carriers 2-2
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G Globodera rostochiensis (golden n
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Poisoning signs and symptoms B-1-6
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Trucks 3-1-17, 3-1-30 Tubers 3-1-3
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