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Golden Nematode Program Manual - Phytosanitary Resources

Golden Nematode Program Manual - Phytosanitary Resources

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2<br />

Procedures<br />

<strong>Golden</strong> <strong>Nematode</strong><br />

<strong>Program</strong> <strong>Manual</strong><br />

1<br />

Laboratory, Rack Room, and Wash<br />

Room<br />

Contents<br />

Introduction page 2-4-1<br />

Work Unit page 2-4-2<br />

Rack Room page 2-4-2<br />

Step 1: Unload the Vehicles page 2-4-3<br />

Step 2: Disinfect and Restock Transport Vehicles page 2-4-4<br />

Step 3: Allow the Samples to Dry page 2-4-5<br />

Step 4: Gather a Sample Collection from the Rack Room page 2-4-7<br />

Sample Wash Room page 2-4-10<br />

Step 1: Gather Materials for Sample Washing page 2-4-10<br />

Step 2: Prepare the Cleaning Solution page 2-4-11<br />

Step 3: Wash Hands and Wear Proper Attire page 2-4-11<br />

Step 4: Prepare Sample Bags and Beakers for Sample Washing page 2-4-13<br />

Step 5: Wash the Mineral Soil Samples page 2-4-15<br />

Step 6: Wash the Muck Soil Samples page 2-4-18<br />

Step 7: Place Beakers on Laboratory Counter page 2-4-20<br />

Step 8: Clean and Disinfect the Sample Washroom page 2-4-21<br />

Laboratory Sample Reading page 2-4-22<br />

Step 1: Set Up the Sample Reading Station page 2-4-23<br />

Step 2: Set Up the <strong>Golden</strong> <strong>Nematode</strong> Cyst Station page 2-4-24<br />

Step 3: Prepare for Sample Reading page 2-4-25<br />

Step 4: Read the Samples page 2-4-27<br />

Step 5: Remove Cysts page 2-4-29<br />

<strong>Golden</strong> <strong>Nematode</strong> Cyst Identification page 2-4-31<br />

Record of Infestation Folder page 2-4-32<br />

Introduction<br />

The Laboratory, Rack Room, Wash Room section provides the work unit<br />

procedures for unloading and stacking soil sample bags, washing the<br />

soil samples, examining the samples, and preparing suspect cysts for<br />

positive identification.<br />

11/2007-01 <strong>Golden</strong> <strong>Nematode</strong> <strong>Program</strong> <strong>Manual</strong> 2-4-1<br />

PPQ

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