Golden Nematode Program Manual - Phytosanitary Resources

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Procedures: Preparation, Disinfection, and Clean-up Sanitation Photo by Dan Kepich FIGURE 2-2-8 Pressure Washing Equipment and Supplies Steam Heat Treatment Steam at a temperature of 212° F will destroy, in a short period of exposure, most pathogenic microorganisms of the common vegetative forms or the spore types when in the growing or vegetative state. The steam jet method can be used for sterilizing equipment, since this method takes advantage of the considerable latent heat liberated when steam condenses into water. If the necessary degree of heat is generated in all parts of the material, then the steam jet method is effective for quarantine purposes. Live steam from a jet or nozzle is forced into or through a more or less loose and open mass of material in such amount and for such period required to raise the temperature of all parts of the mass to approximately 212°F. See the PPQ Treatment Manual for more information about steam heat treatment at http://www.aphis.usda.gov/import_export/plants/ manuals/ports/treatment.shtml. 2-2-22 Golden Nematode Program Manual 07/2008-01 PPQ
Procedures: Preparation, Disinfection, and Clean-up Work Unit Clean-up Work Unit Clean-up After the samples have been placed in the Work Unit Rack Room, then finish clean-up and disinfection of vehicles and prepare for the next survey day. Crew Leader Responsibilities After the samples are unloaded into the Work Unit Rack Room, the crew leader will do as follows: 1. Supervise the cleaning and disinfection of survey transport vehicle used during day. 2. Supervise or assist the restocking of supplies in the transport vehicle. 3. Make a copy of the day’s completed PPQ Form 312, Cyst Nematode Survey 4. Give the completed PPQ Form 312 to the PHSS or PPQ Technician at the end of each day. (The technician will give the form to the PHSS to review and make sure all blocks are completed. The PHSS will give the form to the Laboratory Leader at end of each day.) 5. Obtain the next day’s maps from the PPQ Technician. 6. Make sure the vehicle is clean and has been washed at a car wash to remove soil from the undercarriage. Survey Crew Responsibilities After unloading the samples from the vehicle and placing them in the Work Unit Rack Room, the survey crew will do as follows: 1. Sweep the Rack Room. 2. Clean and disinfect inside the vehicle(s), 3. Prepare the transport vehicle for the next day’s use. 07/2008-01 Golden Nematode Program Manual 2-2-23 PPQ
Page 1 and 2: United States Department of Agricul
Page 3 and 4: Contents 1 Golden Nematode Program
Page 5 and 6: List of Figures 1 Golden Nematode P
Page 7 and 8: List of Figures FIGURE A-1-14 Exam
Page 9 and 10: List of Tables 1 Golden Nematode Pr
Page 11 and 12: 1 Introduction Golden Nematode Prog
Page 13 and 14: Introduction Users Users This manua
Page 15 and 16: Introduction Conventions Convention
Page 17 and 18: Introduction Conventions subordinat
Page 19 and 20: 2 Procedures Golden Nematode Progra
Page 21 and 22: Procedures: Introduction Map of Qua
Page 23 and 24: Procedures: Introduction General Pe
Page 25 and 26: Procedures: Introduction General Pe
Page 29 and 30: Procedures: Preparation, Disinfecti
Page 47: Procedures: Preparation, Disinfecti
Page 53 and 54: Procedures: Survey Soil Surveys Soi
Page 55 and 56: Procedures: Survey Systematic Manua
Page 57 and 58: Procedures: Survey Mechanical (Whee
Page 59 and 60: Procedures: Survey Selected Area So
Page 61 and 62: Procedures: Survey Survey Times and
Page 63 and 64: Procedures: Survey Surveys Outside
Page 67 and 68: Procedures: Laboratory, Rack Room,
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Procedures: Regulatory Treatment an
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4 Procedures Golden Nematode Progra
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Procedures: Control Non-chemical Co
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A Appendix Golden Nematode Program
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Appendix A: Forms and Worksheets Go
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Appendix A: Forms and Worksheets Sa
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Appendix A: Forms and Worksheets PP
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B Appendix Golden Nematode Program
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Appendix B: Fumigant Monitoring B.
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Appendix B: First Aid Rescue Breat
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C Appendix Golden Nematode Program
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Appendix C: Maintenance of the Manu
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Appendix C: Maintenance of the Manu
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Glossary 5 Golden Nematode Program
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Glossary: Definitions, Terms, and
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Index 1 C Canada 3-1-4 Carriers 2-2
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G Globodera rostochiensis (golden n
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Poisoning signs and symptoms B-1-6
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Trucks 3-1-17, 3-1-30 Tubers 3-1-3
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